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Cat. No.: HY-P84756A

HIF-2 alpha Antibody (YA4453)(PBS only)

Name: HIF-2 alpha Antibody (YA4453)(PBS only)
Brand: MedChemExpress
SKU: HY-P84756A
Availability: PreOrder

(Synonyms: HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73)

HIF-2 alpha Antibody (YA4453) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HIF-2 alpha.

For research use only. We do not sell to patients.

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	IHC-P IHC-P: Immunohistochemistry-Paraffin	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	FC FC: Flow Cytometry	ELISA ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio	1:200-1:1000	1:200-1:1000	1:200-1:400	1:10000

Description

HIF-2 alpha Antibody (YA4453) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HIF-2 alpha.

Host Mouse
Species Reactivity
Human
Observed Molecular Weight

Observed band size: 96 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 96 kDa

Immunogen

Purified recombinant fragment of human HIF2a (AA: 680-870) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Isotype

IgG1

Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Function

HIF-2 alpha transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD
Subcellular Localization

Nucleus; Nucleus speckle
Expression

Tissue_specificity:It is expressed in most tissues, with the highest expression levels in the placenta, lungs, and heart. It is selectively expressed in endothelial cells.
Subunit

Interacts with HIF3A (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:16181639). Interacts with CREBBP (By similarity). Interacts with EGLN1. Interacts with VHL (PubMed:19208626)
SwissProt ID

Q99814
Gene ID

2034 [NCBI]
Synonyms

HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73