MCM3 Antibody (YA1137)(PBS only)

Cat. No.: HY-P81392A: Data Sheet User Guide for Antibodies
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MCM3 Antibody (YA1137) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MCM3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MCM3 Antibody (YA1137) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MCM3.

Host

Rabbit

Molecular Weight

Predicted band size: 91 kDa;

Observed band size: 100 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P25205

Gene ID

4172 [NCBI]

Immunogen

A synthesized peptide derived from human MCM3

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). Required for the entry in S phase and for cell division (Probable)

Subcellular Localization：Nucleus; Chromosome

Isoforms & Post-Translational Modification：P25205 has 2 isomers: P25205-1: 90981 Da (predicted); P25205-2: 95908 Da (predicted).
Acetylated by MCM3AP;O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner

Subunit：Component of the MCM2-7 complex (PubMed:16899510, PubMed:17296731). The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (PubMed:16899510, PubMed:17296731, PubMed:32453425, PubMed:34694004, PubMed:34700328).

Research Field

Epigenetics and Nuclear Signaling

Synonyms

MCM3; HCC5; P1-MCM3; P1.h; RLFB

Documentation

User Guide for Antibodies (1077 KB)