RNF144B (IBRDC2) Antibody (YA8416)
(Synonyms: bA528A10.3; IBRDC2; p53RFP; PIR2)RNF144B (IBRDC2) Antibody (YA8416) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RNF144B (IBRDC2).
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Host:
Mouse
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Isotype:
IgG1
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Application:
ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|
| Dilution Ratio | 1:50-100 | 1:100 |
Product Details
RNF144B (IBRDC2) Antibody (YA8416) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RNF144B (IBRDC2).
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 33.5 kDa
Human recombinant protein fragment corresponding to amino acids 1-256 of human RNF144B produced in E. coli.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RNF144B is an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. Plays a crucial role in maintaining the genomic stability by controlling the degradation of multiple proteins involved in mitotic progression and DNA damage. Regulates epithelial homeostasis by mediating degradation of CDKN1A and isoform 2 of TP63. Plays a regulatory role in innate immunity by negatively regulating IRF3 activation and IFN-beta production. Mechanistically, inhibits TBK1 phosphorylation and 'Lys-63'-linked polyubiquitination independently of its E3 ligase activity. Alternatively, promotes 'Lys-27' and 'Lys-33'-linked ubiquitination of IFIH1/MDA5, promoting selective autophagic degradation of IFIH1/MDA5 to inhibit antiviral response[1][2][3][4].
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Subcellular Localization
Mitochondrion membrane,Cytoplasm
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Expression
Tissue_Specificity: Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis
Induction: Transcriptionally by TP53 or TP63 (PubMed:23128396, PubMed:38685100). By LPS (PubMed:31509299) -
Isoforms & Post-Translational Modification
Q7Z419 has two isomers: Q7Z419-1: 33697 Da (predicted); Q7Z419-2: 23856 Da (predicted).
Auto-ubiquitinated -
Subunit
Interacts with UBE2L3, UBE2L6 and LCMT2, as well as with BAX
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SwissProt ID
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Synonyms
bA528A10.3; IBRDC2; p53RFP; PIR2
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