RNF144B (IBRDC2) Antibody (YA8416)

Cat. No.: HY-P88732: Data Sheet User Guide for Antibodies
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RNF144B (IBRDC2) Antibody (YA8416) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RNF144B (IBRDC2).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RNF144B (IBRDC2) Antibody (YA8416) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RNF144B (IBRDC2).

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 33.5 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q7Z419

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-256 of human RNF144B produced in E. coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-100
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. Plays a crucial role in maintaining the genomic stability by controlling the degradation of multiple proteins involved in mitotic progression and DNA damage (PubMed:38685100). Regulates epithelial homeostasis by mediating degradation of CDKN1A and isoform 2 of TP63 (PubMed:23128396). Plays a regulatory role in innate immunity by negatively regulating IRF3 activation and IFN-beta production. Mechanistically, inhibits TBK1 phosphorylation and 'Lys-63'-linked polyubiquitination independently of its E3 ligase activity (PubMed:31509299). Alternatively, promotes 'Lys-27' and 'Lys-33'-linked ubiquitination of IFIH1/MDA5, promoting selective autophagic degradation of IFIH1/MDA5 to inhibit antiviral response (PubMed:39285245)

Subcellular Localization：Mitochondrion membrane,Cytoplasm

Expression：
Tissue_Specificity: Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis
Induction: Transcriptionally by TP53 or TP63 (PubMed:23128396, PubMed:38685100). By LPS (PubMed:31509299)

Isoforms & Post-Translational Modification：Q7Z419 has two isomers: Q7Z419-1: 33697 Da (predicted); Q7Z419-2: 23856 Da (predicted).
Auto-ubiquitinated

Subunit：Interacts with UBE2L3, UBE2L6 and LCMT2, as well as with BAX

Synonyms

bA528A10.3; IBRDC2; p53RFP; PIR2

Documentation

User Guide for Antibodies (1077 KB)