Sumo 2+3 Antibody (YA6999)

Cat. No.: HY-P87316: Data Sheet User Guide for Antibodies
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Sumo 2+3 Antibody (YA6999) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Sumo 2+3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Sumo 2+3 Antibody (YA6999) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Sumo 2+3.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 11-12kDa;

Observed band size: 16kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P55854

Immunogen

A synthesized peptide derived from human Sumo 2. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200
FC FC: Flow Cytometry	1ug/Test
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 (PubMed:11451954, PubMed:18538659, PubMed:21965678). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744)

Subcellular Localization：Cytoplasm,Nucleus,Nucleus, PML body

Expression：
Tissue_Specificity: Expressed predominantly in liver

Isoforms & Post-Translational Modification：P55854 has two isomers: P55854-1: 11637 Da (predicted); P55854-2: 15768 Da (predicted).
Polymeric chains can be formed through Lys-11 cross-linking丨Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function

Subunit：Covalently attached to a number of proteins

Synonyms

HSMT3 antibody; MGC117191 antibody; OTTHUMP00000115275 antibody; OTTHUMP00000115276 antibody; OTTHUMP00000115277 antibody; Sentrin 2 antibody; Small ubiquitin like modifier 2 antibody; Small ubiquitin related modifier 2 antibody; small ubiquitin-like modifier 3 antibody; small ubiquitin-related modifier 3 antibody

Documentation

User Guide for Antibodies (1077 KB)