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Art. -Nr.: HY-D2955

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BC‐AZIDE

Name: BC‐AZIDE
Brand: MedChemExpress
SKU: HY-D2955
Availability: PreOrder

(Synonyms: CLIP-AZIDE)

BC‐AZIDE (CLIP-AZIDE) is a CLIP-tag substrate that forms a covalent irreversible linkage with CLIP-tag proteins. When paired with conjugated fluorophores, BC‐AZIDE enables specific labeling of CLIP-tagged proteins in live and fixed cells. BC‐AZIDE can be used for research related to bioorthogonal click chemistry and intracellular orthogonal labeling.

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CAS. Nr.: 2432950-51-9
Formel: C₁₈H₂₃N₇O₄
Molecular Weight:401.42

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (266 KB)

BC‐AZIDE Chemical Structure

CAS. Nr.: 2432950-51-9

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Use of MCE Products

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

BC-AZIDE is azide-functionalized bacterial cellulose modified with an azidotrimethoxysilane anchoring unit; XPS measurements reveal new signals at 399.5 eV (N 1s), 152.71 eV (Si 2s), and 101.8 eV (Si 2p)^[1].
BC-AZIDE (30 min) mediates SPAAC labeling of the nuclear envelope and actin fibers by DBCO-PEG4-5/6-TAMRA in live HeLa cells, enables SPAAC/IEDDA dual labeling of SNAP-/CLIP-tag fusion proteins in live cells with no obvious cross-reactivity, and also accomplishes two-color SPAAC/IEDDA labeling of CLIP-tag targets in fixed-permeabilized HeLa cells^[2]^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)

Chemical Information

CAS. Nr. 2432950-51-9
Molecular Weight 401.42
Formel C₁₈H₂₃N₇O₄
SMILES

[N-]=[N+]=NCCOCCOCC(NCC1=CC=C(C=C1)COC2=NC(N)=CC=N2)=O
Synonyms

CLIP-AZIDE
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (266 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Gayathri V, et al. Functionalization of Bacterial Cellulose and Related Surfaces Using a Facile Coupling Reaction by Thermoresponsive Catalyst. ACS biomaterials science & engineering. 2023 Feb 13;9(2):625-641.

[2]. Krishnamurthy M, et al. Improved Hydrophobicity of a Bacterial Cellulose Surface: Click Chemistry in Action. ACS biomaterials science & engineering. 2020 Feb 10;6(2):879-888.

[3]. Macias-Contreras M, et al. SNAP/CLIP-Tags and Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)/Inverse Electron Demand Diels-Alder (IEDDA) for Intracellular Orthogonal/Bioorthogonal Labeling. Bioconjugate chemistry. 2020 May 20;31(5):1370-1381. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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MOQ

Minimum order quantity

100 mg

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