Cat. No.: HY-D1451

PKH 26

38 Cited Publications

Customer Review

Based on 38 publication(s) in Google Scholar

PKH 26 is a red fluorescent dye, PKH 26 can stably bind to the lipid region of cell membrane and emit red fluorescence (Ex/Em=551/567 nm), which is mainly used for in vitro cell labeling, in vitro cell proliferation studies and in vivo and in vitro cell tracing studies.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 154214-55-8
Formula: C₅₉H₉₇IN₂
Molecular Weight:961.32

Storage:

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

PKH 26 Chemical Structure

CAS No.: 154214-55-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (38)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of PKH 26 working solution
1.1 Preparation of stock solution
Prepare a 1 mM stock solution using DMSO
1.2 Preparation of PKH 26 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain a working solution of 5-10 μM.
Note: Please adjust the concentration of the PKH 26 working solution as needed
2. Cell staining
2.1 Suspended cells (6-well plate)
a. Centrifuge at 4°C for 1000g for 3-5 minutes, discard the supernatant, wash twice with PBS, each time for 5 minutes. The cell density is 1×10⁶/mL.
b. Add 1 mL of the working solution, incubate at room temperature for 10-45 minutes.
c. Centrifuge at 400g for 4-5 minutes at 4°C, discard the supernatant.
d. Wash twice with PBS, each time for 5 minutes.
e. Resuspend the cells in serum-free cell culture medium or PBS. Observe with a fluorescence microscope or flow cytometer.
2.2 Attached cells
a. Cultivate attached cells on a sterile cover glass.
b. Remove the cover glass from the culture medium and aspirate the excess medium.
c. Add 100 μL of the working solution, gently shake to fully cover the cells, incubate at room temperature for 5-30 minutes.
d. Wash twice with the culture medium, each time for 5 minutes. Observe with a fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

567

Excitation (Ex)

551

Chemical Information

CAS No. 154214-55-8
Appearance Solid
Molecular Weight 961.32
Formula C₅₉H₉₇IN₂
Color Brown to reddish brown
SMILES

CC1(C)C(/C=C/C=C2N(CCCCCCCCCCCCCC)C3=C(C=CC=C3)C/2(C)C)=[N+](CCCCCCCCCCCCCCCCCCCCCC)C4=C1C=CC=C4.[I-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Publications (38)

All Cell Imaging/Staining

Sort by:

Journal Impact Factor

Cancer Cell

B cells disrupt tertiary lymphoid structure formation and suppress anti-tumor immunity. [Abstract]

2026 Jan 8:S1535-6108(25)00545-8. PMID: 41512868
Mol Cancer

GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis. [Abstract]

2025 May 26;24(1):151. PMID: 40414849
Mol Cancer

ADAR1-high tumor-associated macrophages induce drug resistance and are therapeutic targets in colorectal cancer. [Abstract]

2025 Apr 16;24(1):116. PMID: 40241135
Adv Mater

A Targeted Exosome Therapeutic Confers Both Cfdna Scavenging and Macrophage Polarization for Ameliorating Rheumatoid Arthritis. [Abstract]

2023 Nov;35(48):e2302503. PMID: 37681753
Nat Commun

Adipose progenitor cell-derived extracellular vesicles suppress macrophage M1 program to alleviate midlife obesity. [Abstract]

2025 Mar 20;16(1):2743. PMID: 40113754

PKH 26 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Mar 20;16(1):2743. [Abstract]

The engulfment of PKH26-labeled EVs (red) by APCs, adipocytes and macrophages was detected by confocal microscopy.
Adv Sci (Weinh)

MicroRNA-Enriched Plant-Derived Exosomes Alleviate Colitis by Modulating Systemic Immunity, Metabolic Homeostasis, and Gut Microbiota. [Abstract]

2025 Sep 24:e05921. PMID: 40994128

PKH 26 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 24:e05921. [Abstract]

Representative fluorescence microscopy images depicting the time‐dependent cellular uptake of CAEs in NCM460 cells at 1‐, 2‐, 4‐, and 6‐h incubation (These histogram bars served as a negative reference for gating PKH 26‐positive cells).

PKH 26 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 24:e05921. [Abstract]

NCM460 cells after 6‐h treatment.
Adv Sci (Weinh)

Rational Design and Organoid-Based Evaluation of a Cocktail CAR-γδ T Cell Therapy for Heterogeneous Glioblastoma. [Abstract]

2025 Mar 20:e2501772. PMID: 40112194
Theranostics

iRGD-TRP-PK1-modified red blood cell membrane vesicles as a new chemotherapeutic drug delivery and targeting system in head and neck cancer. [Abstract]

2025 Jan 1;15(1):86-102. PMID: 39744235
Chem Eng J

2025 Jul 15.
J Nanobiotechnology

CAT/SOD-Enriched Achyranthes bidentata nanovesicles mitigate TMJOA via ROS scavenging and JNK/FOXO1 pathway Inhibition. [Abstract]

2025 Dec 23;23(1):782. PMID: 41430692

PKH 26 purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Dec 23;23(1):782. [Abstract]

Macrophage uptake of PKH26 (24 h)-labeled ABNVs (red).

PKH 26 purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Dec 23;23(1):782. [Abstract]

PKH26 (24 h)-labeled ABNVs were efficiently internalized by chondrocytes.
J Nanobiotechnology

Exosomes derived from platelet-rich plasma alleviate synovial inflammation by enhancing synovial lymphatic function. [Abstract]

2025 Jul 16;23(1):522. PMID: 40671121
J Nanobiotechnology

A fibrin gel-loaded Gouqi-derived nanovesicle (GqDNV) repairs the heart after myocardial infarction by inhibiting p38 MAPK/NF-κB p65 pathway. [Abstract]

2025 Jul 22;23(1):535. PMID: 40696398
J Nanobiotechnology

Gouqi-derived nanovesicles (GqDNVs) inhibited dexamethasone-induced muscle atrophy associating with AMPK/SIRT1/PGC1α signaling pathway. [Abstract]

2024 May 22;22(1):276. PMID: 38778385
Mater Today Bio

Targeted inhibition of PARP-1 in pulmonary epithelial cells and macrophages via SPA-functionalized microparticles attenuates sepsis-induced lung injury. [Abstract]

2026 Feb 25:37:102955. PMID: 41809387
Int J Surg

Apoptotic extracellular vesicles from peripancreatic adipose-derived mesenchymal stem cells ameliorate severe acute pancreatitis through the transcription factor EB-mediated autophagy-lysosomal pathway: an experimental study. [Abstract]

2025 Dec 17. PMID: 41403290
J Cachexia Sarcopenia Muscle

C2C12-Derived ApoVs Promote Skeletal Muscle Development and Ameliorate Age-Related Muscle Loss Through Igf1r/PI3K/AKT/mTOR Pathway. [Abstract]

2025 Dec;16(6):e70159. PMID: 41344911
Nano Res

2024 Aug 13;17:9898–9907.
Oncogene

sEV-mediated intercellular transformation from MGAT4AHigh to MGAT4ALow tumor cells via the HOTAIRM1/miR-196b-5p axis promotes apoptosis resistance in CTCL. [Abstract]

2025 Mar 28. PMID: 40155530
Antioxidants (Basel)

Kynurenic Acid/GPR35 Signaling Protects the Infarcted Heart by Suppressing Macrophage mtDNA-Triggered cGAS-STING Activation. [Abstract]

2026 Feb 27;15(3):300. PMID: 41897447
Pharmaceutics

Small Molecule Cocktails Promote Fibroblast-to-Leydig-like Cell Conversion for Hypogonadism Therapy. [Abstract]

2023 Oct 13;15(10):2456. PMID: 37896216
CNS Neurosci Ther

Dual-Functionalized Extracellular Vesicles Promote Brain Repair and Remodeling Following Ischemic Stroke in Mice. [Abstract]

2025 Sep;31(9):e70597. PMID: 40955109
Int J Mol Sci

The Role of Macrophage Efferocytosis in the Pathogenesis of Apical Periodontitis. [Abstract]

2024 Mar 29;25(7):3854. PMID: 38612664
Toxicology

Role of neuroglia cell-derived microvesicles in cobalt chloride-induced mitochondrial autophagy in neurons. [Abstract]

2025 Jun 7:154214. PMID: 40490208
J Ovarian Res

Human umbilical cord mesenchymal stem cell-derived extracellular vesicles harboring IGF-1 improve ovarian function of mice with premature ovarian insufficiency through the Nrf2/HO-1 pathway. [Abstract]

2024 Nov 14;17(1):224. PMID: 39543679
FASEB J

GSK-3β/β-catenin pathway plays crucial roles in the regulation of NK cell cytotoxicity against myeloma cells. [Abstract]

2023 Mar;37(3):e22821. PMID: 36794671
Cell Signal

Exosomal miR-382-5p prevents pre-metastatic niche formation by inhibiting GPR176/GNAS-CXCR1/CXCR2 axis in colorectal cancer liver metastasis. [Abstract]

2025 Jun 25:111963. PMID: 40578589
Mol Cell Biochem

Upregulation of YY1 in M2 macrophages promotes secretion of exosomes containing hsa-circ-0000326 via super-enhancers to facilitate prostate cancer progression. [Abstract]

2025 Jun;480(6):3873-3888. PMID: 39960585
Heliyon

Small extracellular vesicles derived from miRNA-486 overexpressed dental pulp stem cells mitigate high altitude pulmonary edema through PTEN/PI3K/AKT/eNOS pathway. [Abstract]

2025 Jan 14;11(2):e41960. PMID: 39906863
Anim Reprod Sci

2026 Jul:290:108180. PMID: 41955652
Cell Stress Chaperones

COL5A2-mediated endoplasmic reticulum stress promotes macrophage M2 polarization in lung adenocarcinoma. [Abstract]

2025 May 7:100081. PMID: 40345640
Organogenesis

Regulation of Interferon-β-Modified Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes in Proliferation and Apoptosis of Prostate Cancer Cells. [Abstract]

2023 Dec 31;19(1):2285836. PMID: 38031805
J Neuroimmunol

Exosomes derived from neural stem cells contribute to cerebral ischemia/reperfusion injury via inhibiting autophagy in rats. [Abstract]

2026 Apr:413:578875. PMID: 41619451
Immunobiology

Exosomes derived from baicalin-pretreated bone marrow mesenchymal stem cells inactivate the TLR4/MyD88/NF-kB pathway to improve asthma. [Abstract]

2025 Jul 16;230(4):153103. PMID: 40695068
Oncol Lett

Exosome‑delivered miR‑486‑3p inhibits the progression of osteosarcoma via sponging CircKEAP1/MARCH1 axis components. [Abstract]

2023 Nov 16;27(1):24. PMID: 38058466
bioRxiv

BLOC1S1 regulates autolysosomal and exosomal dynamics during CD4+ T cell differentiation. [Abstract]

2026 May 18:2026.05.14.725149. PMID: 42239123
bioRxiv

2025 Nov 4.
Research Square Preprint

2023 Apr 12.
Research Square Print

2022 Aug.

Solvent & Solubility

In Vitro:

DMSO : 1.67 mg/mL (1.74 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0402 mL

5.2012 mL

10.4024 mL

5 mM

---

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

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Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Fischer K, et al. The flow cytometric PKH-26 assay for the determination of T-cell mediated cytotoxic activity. Methods. 2003;31(2):135-142. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.0402 mL	5.2012 mL	10.4024 mL	26.0059 mL

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