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Art. -Nr.: HY-188058

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Biotin-14-CTP

Biotin-14-CTP is a modified cytidine triphosphate analog, as well as a non-terminating, non-processable nucleotide analog and labeling reagent. Biotin-14-CTP incorporates into nascent RNA strands during transcription elongation, enabling immunolabeling-based visualization of transcription sites. The incorporation of Biotin-14-CTP is inhibited by α-Amanitin (HY-19610), indicating that it can be utilized by RNA polymerase II.

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CAS. Nr.: 208403-22-9
Formel: C₃₁H₅₄N₇O₁₇P₃S
Molecular Weight:921.78

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Biotin-14-CTP Chemical Structure

CAS. Nr.: 208403-22-9

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

Biotin-14-CTP (100 µM; 15 min) labels transcription sites in permeabilized HeLa cells that are anchored to a nucleoskeleton, as they remain distributed throughout the nucleus after extensive chromatin removal, with some sites fragmenting into smaller clusters during chromatin elution^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 208403-22-9
Molecular Weight 921.78
Formel C₃₁H₅₄N₇O₁₇P₃S
SMILES

O[C@@H]1[C@H](O)[C@@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)O[C@H]1N2C=CC(NCCCCCCNC(CCCCCNC(CCCC[C@@H]3SC[C@@]4(NC(N[C@@]43[H])=O)[H])=O)=O)=NC2=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Iborra FJ, et al. Active RNA polymerases are localized within discrete transcription "factories' in human nuclei. Journal of cell science. 1996 Jun;109 ( Pt 6):1427-36.

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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MOQ

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100 mg

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