BODi-1 

BODi-1 is a fluorescent modulator targeting dsDNA, which binds to dsDNA via a bis-intercalation mechanism (E_x=465 nm, E_m=490 nm). BODi-1 exhibits a fluorescence enhancement effect upon binding to nucleic acids, but its fluorescence intensity, anisotropy and average lifetime decrease at higher dye/DNA ratios. When BODi-1 binds to DNA in liposome complexes, it also shows red-shifted emission spectra, along with reduced quantum yield and average lifetime. BODi-1 does not induce significant DNA conformational changes when the dye/DNA ratio is below 0.01. BODi-1 can be used as a fluorescent probe for the characterization of liposome complexes and FRET studies at this ratio^[1].

BOBO-1 exhibits stable fluorescence intensity and anisotropy (~0.20) in supercoiled and relaxed pUC19 DNA when the dye/DNA base ratio ≤ 0.01^[1].
BOBO-1 maintains stable fluorescence anisotropy (~0.20) in pUC19 DNA/DOTAP lipid complexes when the dye/DNA base ratio is ≤0.005; at higher ratios, its fluorescence intensity and anisotropy decrease^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1202.66

C₄₁H₅₄I₄N₆S₂

169454-13-1

C[N+]1=C(/C=C2C=CN(CCC[N+](C)(C)CCC[N+](C)(C)CCCN3C=C/C(C=C3)=C\C4=[N+](C)C5=C(C=CC=C5)S4)C=C\2)SC6=C1C=CC=C6.[I-].[I-].[I-].[I-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Madeira C, et al. Photophysical behavior of a dimeric cyanine dye (BOBO-1) within cationic liposomes. Photochem Photobiol. 2005;81(6):1450-1459.