Brickellin
Brickellin is an antioxidant agent that can be isolated from Varronia curassavica Jacq.. Brickellin inhibits advanced glycation end products (AGEs) formation, protein crosslinking, and oxidative damage to proteins. Brickellin can be used for the research of diabetes.
For research use only. We do not sell to patients.
- CAS No.: 90357-63-4
- Formula: C20H20O9
- Molecular Weight:404.37
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| KB | ED50 |
>25 μg/mL
Compound: brickellin
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Cytotoxicity against human KB cells after 72 hrs
Cytotoxicity against human KB cells after 72 hrs
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[PMID: 1955888] |
| KB | ED50 |
25 μg/mL
Compound: brickellin
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Cytotoxicity against human KB cells after 72 hrs
Cytotoxicity against human KB cells after 72 hrs
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[PMID: 1955888] |
In Vitro
Brickellin (72 h) does not significantly inhibit the growth of KB cells in vitro, with an ED50 value >25 μg/mL[1].
Brickellin (0.031-0.125 mM; 8 days) significantly inhibits methylglyoxal-induced fluorescent advanced glycation end-product formation in bovine serum albumin in a concentration-dependent manner, with 0.125 mM brickellin reducing AGE formation by 60.8% on day 8[2].
Brickellin (0.031-0.125 mM; 8 days) significantly reduces methylglyoxal-induced formation of dityrosine, N′-formylkynurenine, and kynurenine oxidation markers in bovine serum albumin in a concentration-dependent manner[2].
Brickellin (0.031-0.125 mM; 8 days) inhibits methylglyoxal-induced protein carbonyl formation in bovine serum albumin after 8 days of incubation[2].
Brickellin binds strongly to the SARS-CoV-2 Mpro predicted allosteric site[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 90357-63-4
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Molecular Weight 404.37
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Formula C20H20O9
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SMILES
O=C1C(OC)=C(OC2=CC(OC)=C(OC)C(O)=C12)C=3C=C(OC)C(OC)=CC3O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
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Research Protocol for Metabolic Diseases
AMP-activated protein kinase, AMPK, is a conserved cellular energy sensor that responds to reduced cellular energy status and coordinates metabolism by increasing ATP-generating catabolic pathways while suppressing ATP-consuming anabolic processes. In metabolic disease research, the AMPK pathway is experimentally relevant because it regulates hepatic lipid synthesis, fatty acid oxidation, glucose production, skeletal-muscle glucose disposal, mTORC1-linked biosynthesis, autophagy, mitochondrial homeostasis, and whole-body energy balance. The central pathway logic is that energy stress, metformin, exercise-like stimulation, or direct AMPK activators increase AMPKα Thr172 phosphorylation and downstream substrate phosphorylation, including ACC and RAPTOR. Phosphorylation of ACC suppresses lipogenesis and supports fatty acid oxidation, whereas phosphorylation of RAPTOR suppresses mTORC1 signaling and links cellular energy status to growth and protein synthesis control. The pathway is linked
Purity & Documentation
References
[1]. Arisawa M, et al. Isolation and cytotoxicity of two new flavonoids from Chrysosplenium grayanum and related flavonols. J Nat Prod. 1991;54(3):898-901. [Content Brief]
[2]. Rodrigues WD, et al. In Vitro Antiglycation Potential of Erva-Baleeira (Varronia curassavica Jacq.). Antioxidants (Basel). 2023 Feb 19;12(2):522. [Content Brief]
[3]. Kubra B, Badshah SL, Faisal S, Sharaf M, Emwas AH, Jaremko M, Abdalla M. Inhibition of the predicted allosteric site of the SARS-CoV-2 main protease through flavonoids. J Biomol Struct Dyn. 2023 Oct-Nov;41(18):9103-9120. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Brickellin Related Classifications
HY-N13261 Related Classifications
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