C12-NBD Sphinganine

Cat. No.: HY-141577 Purity: 99.9%: Data Sheet
COA

SDS
Handling Instructions
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C12-NBD Sphinganine is a fluorescent ceramidase substrate. C12-NBD Sphinganine can be used for the measurement of alkaline and neutral ceramidase activity from a variety of sources.

For research use only. We do not sell to patients.

C12-NBD Sphinganine Chemical Structure

CAS No. : 474943-05-0

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

C12-NBD Sphinganine is a fluorescent ceramidase substrate. C12-NBD Sphinganine can be used for the measurement of alkaline and neutral ceramidase activity from a variety of sources^[1]^[2]^[3].

Molecular Weight

661.92

Formula

C₃₆H₆₃N₅O₆

CAS No.

474943-05-0

Appearance

Solid

Color

Light yellow to yellow

SMILES

CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(CCCCCCCCCCCNC1=CC=C([N+]([O-])=O)C2=NON=C12)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMF : 30 mg/mL (45.32 mM; Need ultrasonic and warming)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5108 mL	7.5538 mL	15.1076 mL
5 mM	0.3022 mL	1.5108 mL	3.0215 mL
10 mM	0.1511 mL	0.7554 mL	1.5108 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.9%

Select Batch:

Data Sheet (266 KB) SDS (252 KB)

COA (240 KB)

Handling Instructions (2659 KB)

References

[1]. Rigogliuso S, et al. An active form of sphingosine kinase-1 is released in the extracellular medium as component of membrane vesicles shed by two human tumor cell lines. J Oncol. 2010;2010:509329. [Content Brief]

[2]. Okino N, et al. Expression, purification, and characterization of a recombinant neutral ceramidase from Mycobacterium tuberculosis. Biosci Biotechnol Biochem. 2010;74(2):316-21. [Content Brief]

[3]. Vijai Kumar Reddy T, et al. Design, synthesis and in vitro biological evaluation of short-chain C12-sphinganine and its 1,2,3-triazole analogs as potential antimicrobial and anti-biofilm agents. Eur J Med Chem. 2016 Aug 8;118:98-106. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMF	1 mM	1.5108 mL	7.5538 mL	15.1076 mL	37.7689 mL
	5 mM	0.3022 mL	1.5108 mL	3.0215 mL	7.5538 mL
	10 mM	0.1511 mL	0.7554 mL	1.5108 mL	3.7769 mL
	15 mM	0.1007 mL	0.5036 mL	1.0072 mL	2.5179 mL
	20 mM	0.0755 mL	0.3777 mL	0.7554 mL	1.8884 mL
	25 mM	0.0604 mL	0.3022 mL	0.6043 mL	1.5108 mL
	30 mM	0.0504 mL	0.2518 mL	0.5036 mL	1.2590 mL
	40 mM	0.0378 mL	0.1888 mL	0.3777 mL	0.9442 mL