BC‐AZIDE
BC-AZIDE (CLIP-AZIDE) is a nitrogen-functionalized protein labeling probe based on the CLIP-tag system. BC-AZIDE can be used for bioorthogonal click chemistry and intracellular orthogonal labeling.
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- CAS No.: 2432950-51-9
- Formule: C18H23N7O4
- Masse moléculaire:401.42
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
Chemical Information
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CAS No. 2432950-51-9
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Masse moléculaire 401.42
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Formule C18H23N7O4
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SMILES
[N-]=[N+]=NCCOCCOCC(NCC1=CC=C(C=C1)COC2=NC(N)=CC=N2)=O
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Synonyms
CLIP-AZIDE
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BC‐AZIDE Related Classifications
HY-D2955 Related Classifications
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