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Cat. No.: HY-125273

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DNS-pE

DNS-pE is a 3-phosphoglycerate dehydrogenase (PHGDH) inhibitor with a K_i of 7.4 μM. DNS-pE selectively and irreversibly covalently modifies endogenous PHGDH in live mammalian cells, its vinyl sulfone moiety acts as a fluorescence quencher that becomes highly fluorescent upon covalent modification of PHGDH. DNS-pE can be used as a fluorescent dye for live-cell imaging.

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CAS No.: 2196245-98-2
Formule: C₁₄H₁₅NO₂S
Masse moléculaire:261.34

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (268 KB)

DNS-pE Chemical Structure

CAS No.: 2196245-98-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

In Vitro

DNS-pE (10 μM; 1 h) shows robust fluorescence Turn-ON activity with cysteine, achieving a 21-fold increase in fluorescence after 1 h of incubation at room temperature^[1].
DNS-pE (1-50 μM; 3 h) concentration-dependently compets with a clickable IA probe for binding to endogenous PHGDH in live MCF-7 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

MCF-7 cells
Concentration:

1; 2; 5; 10; 20; 50 μM
Incubation Time:

3 h
Result:

Reduced 56-kDa PHGDH band fluorescence in a concentration-dependent manner.
Competitively binded with a clickable IA probe to endogenous PHGDH in live MCF-7 cells.

Chemical Information

CAS No. 2196245-98-2
Masse moléculaire 261.34
Formule C₁₄H₁₅NO₂S
SMILES

CN(C)C1=CC=C2C=C(S(=O)(C=C)=O)C=CC2=C1
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (268 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Pan S, et al. A Vinyl Sulfone-Based Fluorogenic Probe Capable of Selective Labeling of PHGDH in Live Mammalian Cells. Angew Chem Int Ed Engl. 2018 Jan 8;57(2):579-583. [Content Brief]

Calculators

Calculateur de molarité

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Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Concentration _(final) Concentration _(final)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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