Lyso-MPCB

Guidelines (The following is a recommended operating procedure for guidance only, and adjustments should be made according to your specific requirements)

1. Stock Solution Preparation
1.2 Concentration recommendation: 1 mM.

2. Working Solution Preparation
2.1 Working solution concentration: 10 μM.
2.2 Notes: Adjust the working solution concentration as needed; prepare fresh before use.

3. Staining Procedure
3.1 Sample Type Description
3.1.1 Adherent cells (MCF-7 cells)^[1][2]
3.2 Incubation Conditions
3.2.1 MCF-7 cells: Incubate with 10 μM Lyso-MPCB for 30 min; after staining, incubate the cells in nutrient-free medium for up to 4 h.

5. Detection and Analysis
5.1 Instrument type: Fluorescence microscope.
5.2 Excitation/Emission Wavelengths
5.2.1 E_x (TP) = 760 nm; E_m (blue) = 400-420 nm; E_m (green) = 465-485 nm (dual-channel detection is performed using the blue emission channel and green emission channel).
5.3 Result Analysis
5.3.1 Changes in fluorescence intensity: When lysosomal pH decreases from 8.0 to 3.0, the ratio of green fluorescence intensity to blue fluorescence intensity (I_green/I_blue) decreases; within the pH range of 4.2-5.6, the blue/green fluorescence ratio signal shows a linear relationship with pH; as autophagy induction time extends, both the distribution range and average level of pseudo-color increase.
5.3.2 Fluorescence localization: Specific targeting of lysosomes.
5.3.3 Color change: Emits blue fluorescence under alkaline conditions and green fluorescence under acidic conditions; pseudo-color shift corresponds to changes in lysosomal pH.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.