N-Methyltaurine
Based on 1 Customer Validation
N-Methyltaurine is a taurine-derived metabolite with a minimum effective concentration of 10 μM for maintaining hamster sperm motility. N-Methyltaurine is the dominant osmolyte of Vespermum virulentum.
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- Pureté : 98.0%
- CAS No.: 107-68-6
- Formule: C3H9NO3S
- Masse moléculaire:139.17
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Activité biologique
Description
Chemical Information
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CAS No. 107-68-6
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Appearance Solid
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Masse moléculaire 139.17
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Formule C3H9NO3S
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Color White to off-white
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SMILES
CNCCS(=O)(=O)O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
In Vitro:
H2O : 100 mg/mL (718.55 mM; Need ultrasonic)
DMSO : 6.67 mg/mL (47.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Sperm Thawing for Functional Recovery
Sperm thawing for functional recovery is a post-cryopreservation procedure designed to restore and measure sperm motility, viability, membrane/acrosome integrity, DNA integrity, and usable motile sperm yield after freezing-induced injury; published human studies show that cryopreservation reduces motility and viability and can damage the plasmalemma, acrosome, tail, and DNA integrity. The experimental readout is generated by comparing post-thaw motility recovery, viability, acrosomal status, DNA integrity, and recovered motile sperm after thawing and optional sperm-selection steps; thawing at 40°C improved motility recovery compared with 20-37°C without significant differences in viability, ATP content, acrosomal status, or DNA integrity in one human donor-semen study.
Pureté et documentation
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Fiche technique (271 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 7.1855 mL | 35.9273 mL | 71.8546 mL | 179.6364 mL |
| 5 mM | 1.4371 mL | 7.1855 mL | 14.3709 mL | 35.9273 mL | |
| 10 mM | 0.7185 mL | 3.5927 mL | 7.1855 mL | 17.9636 mL | |
| 15 mM | 0.4790 mL | 2.3952 mL | 4.7903 mL | 11.9758 mL | |
| 20 mM | 0.3593 mL | 1.7964 mL | 3.5927 mL | 8.9818 mL | |
| 25 mM | 0.2874 mL | 1.4371 mL | 2.8742 mL | 7.1855 mL | |
| 30 mM | 0.2395 mL | 1.1976 mL | 2.3952 mL | 5.9879 mL | |
| 40 mM | 0.1796 mL | 0.8982 mL | 1.7964 mL | 4.4909 mL | |
| H2O | 50 mM | 0.1437 mL | 0.7185 mL | 1.4371 mL | 3.5927 mL |
| 60 mM | 0.1198 mL | 0.5988 mL | 1.1976 mL | 2.9939 mL | |
| 80 mM | 0.0898 mL | 0.4491 mL | 0.8982 mL | 2.2455 mL | |
| 100 mM | 0.0719 mL | 0.3593 mL | 0.7185 mL | 1.7964 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.