UB-221
Based on 1 Customer Validation
UB-221 is an anti-IgE monoclonal antibody (human IgE Kd = 5.85×10-11 M). UB-221 blocks the IgE-FcεRI interaction and promotes CD23 binding by binding to free IgE and CD23-bound IgE, inhibits basophil activation and degranulation, stabilizes membrane-bound CD23 on B lymphocytes and downregulates IgE synthesis, and induces apoptosis (Apoptosis) of B cells expressing membrane-bound IgE. UB-221 directs IgE toward non-inflammatory clearance pathways, reduces IgE levels and FcεRI expression, and can be used for research on chronic spontaneous urticaria and food allergy.
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- Pureté : 98.95%
- CAS No.: 2755114-30-6
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
Species Reactivity
Human
IC50 & Target
IGHE
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Ramos | EC50 |
7.9 ng/mL
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Binding to Ramos B cells expressing long-form membrane-bound IgE measured as mean EC50.
Binding to Ramos B cells expressing long-form membrane-bound IgE measured as mean EC50.
|
35912861 |
In Vitro
UB-221 does not trigger ADCC/CDC in IgE-preloaded CD23-overexpressing SKW6.4 cells and does not induce cytokine release from human PBMCs[1].
UB-221 binds IgE that is bound to CD23, and the preformed UB-221-IgE complex binds CD23 in ELISA, with EC50 values of 39.1 and 41.6 ng/mL, respectively[1].
UB-221 blocks FcεRI-mediated RBL SX-38 degranulation with an IC50 of 0.14 μg/mL and does not activate human basophils[1].
UB-221 (0-75,000 ng/mL; 1 hour at room temperature) neutralizes high IgE in human serum samples, with an EC90 of 1,097 ng/mL in the low IgE group and 27,539 ng/mL in the high IgE group[1].
UB-221 inhibits FcεRI interaction and promotes CD23 binding in in vitro IgE receptor interaction assays[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Parmacokinetics
| Species | Dose | Route | T1/2 |
|---|---|---|---|
| Cynomolgus Monkey[1] | 5.0 mg/kg | i.v. | 6.3 day |
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Cynomolgus macaque (cynomolgus monkey; n=3; suitable pharmacology and toxicology animal model)[1]
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Dosage:5.0 mg/kg
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Administration:i.v.; single dose
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Result:Induced a rapid, pronounced reduction in serum free cIgE by 90% to 100%.
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Animal Model:hIGHE-knockin (human IgE ε,κ-knockin; F1 hybrid of male C57BL/6 background crossed with female BALB/c; 8-16 weeks old)[1]
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Dosage:0.3 mg/kg or 3.0 mg/kg
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Administration:i.p.; single dose
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Result:Rapidly reduced high serum free-chimeric-IgE levels by greater than 90%.
Essai clinique
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | 2011-05 | PHASE1 |
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
ELISA, FACS, Functional assay
Chemical Information
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CAS No. 2755114-30-6
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
N/A
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Livraison
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
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Fiche technique (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
UB-221 Related Classifications
HY-P991592 Related Classifications
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