2659241-66-2
Chemical Structure
Lys-VBOD
- CAS No.: 2659241-66-2
- Formula:C37H36BF2N5O2
- Molecular Weight:631.52
InChIKey: FGKPCMDXIRPJHE-ACCUITESSA-N
SMILES: CC1=CC(/C=C/C2=CN(C3=C2C=CC=C3)CCN4CCOCC4)=[N+]5C1=C(C6=C(C=C(N6[B-]5(F)F)C)C)C7=CC=C8C=CC=C(C8=N7)O
Biological Activity: Lys-VBOD is a Fluorescent probe for lysosomal viscosity monitoring and live cell imaging. Its detection mechanism depends on viscosity: increased viscosity restricts the twisting of the double bond between the indole and BODIPY fluorophore, as well as the rotation of the single bond between quinoline and BODIPY, which reduces non-radiative decay and leads to enhanced fluorescence intensity in high viscosity environments; it also features a morpholine group that provides lysosome-targeting properties, allowing it to localize to lysosomes for viscosity detection. The probe has excitation/emission wavelengths of Ex/Em = 602/637 nm, and its fluorescence intensity shows a good linear relationship with log-transformed viscosity values. Lys-VBOD is stable across a wide pH range of 5.35 to 10.74, is not affected by macromolecules and proteins like BSA, has a fluorescence lifetime that increases with viscosity, and can visualize real-time lysosomal viscosity changes in live cells under Dexamethasone (HY-14648) stimulation[1].
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Lys-VBOD | Lys-VBOD is a Fluorescent probe for lysosomal viscosity monitoring and live cell imaging. Its detection mechanism depends on viscosity: increased viscosity restricts the twisting of the double bond between the indole and BODIPY fluorophore, as well as the rotation of the single bond between quinoline and BODIPY, which reduces non-radiative decay and leads to enhanced fluorescence intensity in high viscosity environments; it also features a morpholine group that provides lysosome-targeting properties, allowing it to localize to lysosomes for viscosity detection. The probe has excitation/emission wavelengths of Ex/Em = 602/637 nm, and its fluorescence intensity shows a good linear relationship with log-transformed viscosity values. Lys-VBOD is stable across a wide pH range of 5.35 to 10.74, is not affected by macromolecules and proteins like BSA, has a fluorescence lifetime that increases with viscosity, and can visualize real-time lysosomal viscosity changes in live cells under Dexamethasone (HY-14648) stimulation. | |||||||||||||||||||||
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