Scutellarin
Based on 24 publication(s) in Google Scholar
Scutellarin, an active flavone isolated from Scutellaria baicalensis, can down-regulates the STAT3/Girdin/Akt signaling in HCC cells, and inhibits RANKL-mediated MAPK and NF-κB signaling pathway in osteoclasts. Scutellarin is active against HIV-1IIIB, HIV-1(74V) and HIV-1KM018 with EC50s of 26 μM, 253 μM and 136 μM, respectively.
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- Reinheit: 99.57%
- CAS. Nr.: 27740-01-8
- Formel: C21H18O12
- Molecular Weight:462.36
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
Publications Citing Use of MedChemExpress (MCE) Scutellarin
More- Pharmacol Res. 2025 Jan 15:107605. [Abstract]
- Food Chem. 2025 Dec 30:497:146992. [Abstract]
- Food Chem. 2025 May 31:489:144992. [Abstract]
- Cell Death Dis. 2020 Nov 13;11(11):978. [Abstract]
- Phytomedicine. 2025 May:140:156484. [Abstract]
- J Transl Med. 2025 Feb 17;23(1):195. [Abstract]
- Phytother Res. 2025 Sep 18. [Abstract]
- Phytother Res. 2022 Jan;36(1):433-447. [Abstract]
- Ind Crops Prod. 2025 Dec 11;239:122458.
- Inflammation. 2024 Jun;47(3):853-873. [Abstract]
- Bioengineered. 2022 Jan;13(1):1013-1024. [Abstract]
- J Mater Sci. 2026 Apr 27;61(22):15999-16015.
- Med Oncol. 2025 Nov 25;43(1):24. [Abstract]
- Toxicol Appl Pharmacol. 2025 Oct 9:505:117564. [Abstract]
- Front Biosci (Landmark Ed). 2025 Mar 6;30(3):36255. [Abstract]
- Cell Biol Int. 2022 Oct;46(10):1588-1603. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- PLoS One. 2025 Jan 31;20(1):e0318031. [Abstract]
- Tissue Cell. 2025 Nov 17:99:103234. [Abstract]
- J Nat Med. 2025 Nov 23. [Abstract]
- Tissue Cell. 2024 Apr:87:102300. [Abstract]
- Exp Ther Med. 2023 Feb 17;25(4):155. [Abstract]
- Thorac Cancer. 2019 Mar;10(3):492-500. [Abstract]
- Nephrology (Carlton). 2022 Aug;27(8):690-700. [Abstract]
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Cell Proliferation/Viability Assay
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ELISA
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WB
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Histological Imaging/Staining
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WB
Biologische Aktivität
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STAT3 |
HIV-1 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>50 μM
Compound: 1
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Antiproliferative activity against human A549 cells after 72 hrs by MTT assay
Antiproliferative activity against human A549 cells after 72 hrs by MTT assay
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[PMID: 30243153] |
| Bel-7402 | IC50 |
>50 μM
Compound: 1
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Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay
Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay
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[PMID: 30243153] |
| CHO | IC50 |
63.4 μM
Compound: 3
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Displacement of [3H]LSD from human 5HT7 receptor expressed in CHO cells
Displacement of [3H]LSD from human 5HT7 receptor expressed in CHO cells
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[PMID: 12713409] |
| HCT-116 | IC50 |
77 μM
Compound: Scutellarin
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Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay
Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay
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[PMID: 28458133] |
| HepG2 | IC50 |
56.09 μM
Compound: Scutellarin
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Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay
Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay
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[PMID: 28458133] |
| L02 | IC50 |
>100 μM
Compound: Scutellarin
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Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay
Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay
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[PMID: 28458133] |
| L02 | IC50 |
>50 μM
Compound: 1
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Cytotoxicity against human LO2 cells assessed as decrease in cell viability after 72 hrs by MTT assay
Cytotoxicity against human LO2 cells assessed as decrease in cell viability after 72 hrs by MTT assay
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[PMID: 30243153] |
| MCF7 | IC50 |
>100 μM
Compound: Scutellarin
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Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay
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[PMID: 28458133] |
| MCF7 | IC50 |
>50 μM
Compound: 1
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Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay
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[PMID: 30243153] |
| PC-3 | IC50 |
72.9 μM
Compound: Scutellarin
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Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay
Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay
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[PMID: 28458133] |
Scutellarin treatment significantly reduces HepG2 cell viability in a dose-dependent manner, and inhibits migration and invasion of HCC cells in vitro. Scutellarin treatment significantly reduces STAT3 and Girders of actin filaments (Girdin) expression, STAT3 and Akt phosphorylation in HCC cells. Introduction of STAT3 overexpression restores the scutellarin-downregulated Girdin expression, Akt activation, migration and invasion of HCC cells. Furthermore, induction of Girdin overexpression completely abrogates the inhibition of scutellarin on the Akt phosphorylation, migration and invasion of HCC cells. Scutellarin can inhibit HCC cell metastasis in vivo, and migration and invasion in vitro by down-regulating the STAT3/Girdin/Akt signaling[1]. Scutellarin selectively enhances Akt phosphorylation[2]. Scutellarin is a putative therapeutic agent as it has been found to not only suppress microglial activation thus ameliorating neuroinflammation, but also enhance astrocytic reaction. Acutellarin amplifies the astrocytic reaction by upregulating the expression of neurotrophic factors among others thus indicating its neuroprotective role. Remarkably, the effects of scutellarin on reactive astrocytes are mediated by activated microglia supporting a functional "cross-talk" between the two glial types[3]. Scutellarin can suppress RANKL-mediated osteoclastogenesis, the function of osteoclast bone resorption, and the expression levels of osteoclast-specific genes (tartrate-resistant acid phosphatase (TRAP), cathepsin K, c-Fos, NFATc1). Further investigation indicates that Scutellarin can inhibit RANKL-mediated MAPK and NF-κB signaling pathway, including JNK1/2, p38, ERK1/2, and IκBα phosphorylation[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 27740-01-8
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Appearance Solid
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Molecular Weight 462.36
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Formel C21H18O12
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Color Light yellow to green yellow
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SMILES
O=C(C=C(C1=CC=C(O)C=C1)OC2=CC(O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3C(O)=O)=C4O)C2=C4O
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (24)
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Journal Impact Factor
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Most Recent
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Pharmacol Res
Scutellarin inhibits pyroptosis via selective autophagy degradation of p30/GSDMD and suppression of ASC oligomerization. [Abstract]2025 Jan 15:107605. PMID: 39824372
Scutellarin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Jan 15:107605. [Abstract]
THP-1 and HEK-293T were treated with Scutellarin (SCU) at gradient concentration (50 μM, 100 μM and 200 μM) for 24 h, cell viability was detected by CCK-8 assay.
Scutellarin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Jan 15:107605. [Abstract]
THP-1 cells were primed with 500 ng/mL LPS for 4 h, followed by a 1-hour treatment with varying concentrations of Scutellarin (SCU, 50 μM and 100 μM) and a subsequent 30-minute 10 μM NIG stimulation to activate the NLRP3 inflammasome. The supernatants of THP-1 cells were collected for IL-18 detection using an ELISA kit.
Scutellarin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Jan 15:107605. [Abstract]
FLAG-NLRP3, FLAG-ASC, HA-caspase-1, and FLAG-IL-1β were co-transfected into HEK-293T cells. Five hours later, the culture medium was replaced, and 100 μM Scutellarin (SCU) was added. After 24 hours, the cells underwent DSS cross-linking, and protein samples were collected to detect inflammasome components via Western blotting.
Scutellarin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Jan 15:107605. [Abstract]
Spleens were used to prepare pathological sections and perform HE staining. These sections revealed that Scutellarin (SCU, 10 mg/kg; i.p.) inhibited LPS-induced inflammatory cell infiltration.
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Food Chem
Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]2025 Dec 30:497:146992. PMID: 41285060 -
Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 May 31:489:144992. PMID: 40466530 -
Cell Death Dis
Scutellarin ameliorates pulmonary fibrosis through inhibiting NF-κB/NLRP3-mediated epithelial-mesenchymal transition and inflammation. [Abstract]2020 Nov 13;11(11):978. PMID: 33188176 -
Phytomedicine
Fangchinoline suppresses nasopharyngeal carcinoma progression by inhibiting SQLE to regulate the PI3K/AKT pathway dysregulation. [Abstract]2025 May:140:156484. PMID: 40090046 -
J Transl Med
IFN-γ-mediated inhibition of JAK/STAT signaling via nano-scutellarin treatment is an efficient strategy for ameliorating liver fibrosis. [Abstract]2025 Feb 17;23(1):195. PMID: 39962553 -
Phytother Res
Scutellarin Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Mice by Inhibiting M1 Macrophage Polarization via the GBP2/JAK2/STAT3 Signaling Pathway. [Abstract]2025 Sep 18. PMID: 40968089 -
Phytother Res
Scutellarin ameliorates hepatic lipid accumulation by enhancing autophagy and suppressing IRE1α/XBP1 pathway. [Abstract]2022 Jan;36(1):433-447. PMID: 34859513 -
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Inflammation
Scutellarin Alleviates Ovalbumin-Induced Airway Remodeling in Mice and TGF-β-Induced Pro-fibrotic Phenotype in Human Bronchial Epithelial Cells via MAPK and Smad2/3 Signaling Pathways. [Abstract]2024 Jun;47(3):853-873. PMID: 38168709 -
Bioengineered
Preparation and characterization of scutellarin loaded on ultradeformable nano-liposomes scutellarin EDTMP (S-UNL-E) and in vitro study of its osteogenesis. [Abstract]2022 Jan;13(1):1013-1024. PMID: 34974800 -
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Med Oncol
Scutellarin triggers ferroptosis in ovarian cancer cells via inhibiting AKT/mTOR and JAK2/STAT3 pathways. [Abstract]2025 Nov 25;43(1):24. PMID: 41288840 -
Toxicol Appl Pharmacol
Scutellarin attenuates keloid fibroblast progression by targeting EGFR/PI3K/AKT signaling: An integrated network pharmacology and in vitro experimental study. [Abstract]2025 Oct 9:505:117564. PMID: 41067505 -
Front Biosci (Landmark Ed)
Scutellarin Attenuates Pro-Inflammatory Foam Cell Formation and Facilitates M2 Polarization in Microglia during Copper Homeostasis Imbalance via the MAPK Signaling Pathway. [Abstract]2025 Mar 6;30(3):36255. PMID: 40152387 -
Cell Biol Int
Scutellarin-mediated autophagy activates exosome release of rat nucleus pulposus cells by positively regulating Rab8a via the PI3K/PTEN/Akt pathway. [Abstract]2022 Oct;46(10):1588-1603. PMID: 35762224 -
Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
PLoS One
Scutellarin combined with lidocaine exerts antineoplastic effect in human glioma associated with repression of epidermal growth factor receptor signaling. [Abstract]2025 Jan 31;20(1):e0318031. PMID: 39888904 -
Tissue Cell
Scutellarin reduces apoptosis in R28 cells induced by continuous hydrostatic pressure combined with oxygen-glucose deprivation through the Keap1/Nrf2/NF-κB pathway. [Abstract]2025 Nov 17:99:103234. PMID: 41252784 -
J Nat Med
2025 Nov 23. PMID: 41276774 -
Tissue Cell
Scutellarin alleviates tensile stress-induced proliferation and migration of venous smooth muscle cells via mediating the p38 MAPK pathway. [Abstract]2024 Apr:87:102300. PMID: 38211409 -
Exp Ther Med
Scutellarin ameliorates ischemia/reperfusion injury‑induced cardiomyocyte apoptosis and cardiac dysfunction via inhibition of the cGAS‑STING pathway. [Abstract]2023 Feb 17;25(4):155. PMID: 36911381 -
Thorac Cancer
Scutellarin suppresses proliferation and promotes apoptosis in A549 lung adenocarcinoma cells via AKT/mTOR/4EBP1 and STAT3 pathways. [Abstract]2019 Mar;10(3):492-500. PMID: 30666790
Scutellarin purchased from MedChemExpress. Usage Cited in: Thorac Cancer. 2019 Mar;10(3):492-500. [Abstract]
A549 cells are treated with Scutellarin (SCU) (0, 200, 400, 600 μM) for 24 hours. Western blotting analyses are used to detect the expression of proteins in the STAT3 pathways, cyclin D1, and cyclin E.
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Nephrology (Carlton)
2022 Aug;27(8):690-700. PMID: 35638402
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (216.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 10 mg/mL (21.63 mM); Suspended solution; Need ultrasonic
Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 5 mg/mL (10.81 mM); Suspended solution; Need ultrasonic
Protokoll
HepG2 cells (1×105/well) are cultured in 96-well plates and treated in triplicate with scutellarin at concentrations of 5, 10, 20, 30, and 100 μM or vehicle alone for 24 h. The cellular viability is tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and is expressed as a percentage of proliferation versus controls.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
To establish an orthotopic liver xenograft model, individual mice are anesthetized with isoflurane and a small incision is made in their abdomen. Individual mice are injected with 2×106 SK-Hep1 cells in 30 μL Matrigel into their left lobe of the liver. Twenty-four hours after orthotopic liver implantation, the mice are randomized and injected intraperitoneally with scutellarin (50 mg/kg/day) or vehicle (0.9% NaCl, normal saline) daily for 35 consecutive days (n=10 per group). Subsequently, the mice are sacrificed, and their lungs and livers are excised, fixed in 10% buffered formalin and paraffin-embedded for hematoxylin and eosin staining.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (282 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Ke Y, et al. Scutellarin suppresses migration and invasion of human hepatocellular carcinoma by inhibiting the STAT3/Girdin/Akt activity. Biochem Biophys Res Commun. 2016 Dec 18. pii: S0006-291X(16)32174-X [Content Brief]
[2]. Yang LL, et al. Differential regulation of baicalin and scutellarin on AMPK and Akt in promoting adipose cell glucose disposal. Biochim Biophys Acta. 2016 Nov 27;1863(2):598-606. [Content Brief]
[3]. Wu CY, et al. Scutellarin attenuates microglia-mediated neuroinflammation and promotes astrogliosis in cerebral ischemia - a therapeutic consideration. Curr Med Chem. 2016 Nov 18. [Epub ahead of print] [Content Brief]
[4]. Li Q, et al. Scutellarin attenuates vasospasm through the Erk5-KLF2-eNOS pathway after subarachnoid hemorrhage in rats. J Clin Neurosci. 2016 Dec;34:264-270 [Content Brief]
[5]. Zhao S, et al. Scutellarin inhibits RANKL-mediated osteoclastogenesis and titanium particle-induced osteolysis via suppression of NF-κB and MAPK signaling pathway. Int Immunopharmacol. 2016 Nov;40:458-465 [Content Brief]
[6]. Zhang GH, et al. The anti-HIV-1 effect of scutellarin. Biochem Biophys Res Commun. 2005 Sep 2;334(3):812-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1628 mL | 10.8141 mL | 21.6282 mL | 54.0704 mL |
| 5 mM | 0.4326 mL | 2.1628 mL | 4.3256 mL | 10.8141 mL | |
| 10 mM | 0.2163 mL | 1.0814 mL | 2.1628 mL | 5.4070 mL | |
| 15 mM | 0.1442 mL | 0.7209 mL | 1.4419 mL | 3.6047 mL | |
| 20 mM | 0.1081 mL | 0.5407 mL | 1.0814 mL | 2.7035 mL | |
| 25 mM | 0.0865 mL | 0.4326 mL | 0.8651 mL | 2.1628 mL | |
| 30 mM | 0.0721 mL | 0.3605 mL | 0.7209 mL | 1.8023 mL | |
| 40 mM | 0.0541 mL | 0.2704 mL | 0.5407 mL | 1.3518 mL | |
| 50 mM | 0.0433 mL | 0.2163 mL | 0.4326 mL | 1.0814 mL | |
| 60 mM | 0.0360 mL | 0.1802 mL | 0.3605 mL | 0.9012 mL | |
| 80 mM | 0.0270 mL | 0.1352 mL | 0.2704 mL | 0.6759 mL | |
| 100 mM | 0.0216 mL | 0.1081 mL | 0.2163 mL | 0.5407 mL |