tCFA15
Based on 1 Customer Validation
tCFA15 is a trimethyl cyclohexenonic long chain fatty alcohol containing 15 carbon atoms on the side chain, promotes the differentiation of neurons, and may regulates Notch signaling.
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- Reinheit: 99.12%
- CAS. Nr.: 220757-88-0
- Formel: C24H44O2
- Molecular Weight:364.60
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Speicherung:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
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Neuronal Signaling Compound Library
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Wnt/Hedgehog/Notch Compound Library
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Biologische Aktivität
Notch1[1]
tCFA15 (10 nM-1 μM) dose-dependently affects the differentiation of neural stem cell-derived neurospheres by promoting neurons at the expense of astrocytes, and such effect is via Notch1. tCFA15 (1 μM) specifically decreases the level of Notch1 mRNA in spheres, and such an effect is also observed in decreases Notch1 expression in neuronal and glial cell cultures[1]. tCFA15 stimulates arginine vasopressin secretion in nerve terminals of the neurohypophysis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 220757-88-0
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Appearance Oil
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Molecular Weight 364.60
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Formel C24H44O2
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Color Light yellow to yellow
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SMILES
O=C1C(C)=C(CCCCCCCCCCCCCCCO)C(C)(C)CC1
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
DMSO : 130 mg/mL (356.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.17 mg/mL (5.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (266 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Bouissac J, et al. tCFA15, a trimethyl cyclohexenonic long-chain fatty alcohol, affects neural stem fate and differentiation by modulating Notch1 activity. Eur J Pharmacol. 2013 Oct 15;718(1-3):383-92. [Content Brief]
[2]. Girlanda-Junges C, et al. 3-(15-Hydroxypentadecyl)-2,4,4-trimethyl-2-cyclohexen-1-one and its effect on neuropeptide secretion. Bioorg Med Chem Lett. 2000 Nov 20;10(22):2537-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7427 mL | 13.7137 mL | 27.4273 mL | 68.5683 mL |
| 5 mM | 0.5485 mL | 2.7427 mL | 5.4855 mL | 13.7137 mL | |
| 10 mM | 0.2743 mL | 1.3714 mL | 2.7427 mL | 6.8568 mL | |
| 15 mM | 0.1828 mL | 0.9142 mL | 1.8285 mL | 4.5712 mL | |
| 20 mM | 0.1371 mL | 0.6857 mL | 1.3714 mL | 3.4284 mL | |
| 25 mM | 0.1097 mL | 0.5485 mL | 1.0971 mL | 2.7427 mL | |
| 30 mM | 0.0914 mL | 0.4571 mL | 0.9142 mL | 2.2856 mL | |
| 40 mM | 0.0686 mL | 0.3428 mL | 0.6857 mL | 1.7142 mL | |
| 50 mM | 0.0549 mL | 0.2743 mL | 0.5485 mL | 1.3714 mL | |
| 60 mM | 0.0457 mL | 0.2286 mL | 0.4571 mL | 1.1428 mL | |
| 80 mM | 0.0343 mL | 0.1714 mL | 0.3428 mL | 0.8571 mL | |
| 100 mM | 0.0274 mL | 0.1371 mL | 0.2743 mL | 0.6857 mL |
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