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Cat. No.: HY-111545

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BSc5371

BSc5371 is a potent and irreversible FLT3 inhibitor, with K_ds of 1.3, 0.83, 1.5, 5.8 and 2.3 nM for mutant FLT3(D835H), FLT3(ITD, D835V), FLT3(ITD, F691L), FLT3-ITD and wild type FLT3wt, respectively. BSc5371 is cytotoxic to FLT3-dependent cell lines.

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CAS No.: 2286419-03-0
Formule: C₂₄H₃₁N₅O₄S
Masse moléculaire:485.60

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (268 KB)

BSc5371 Chemical Structure

CAS No.: 2286419-03-0

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Protocole (1)
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

Kd: 1.3 nM (LT3(D835H)), 0.83 nM (LT3(ITD, D835V)), 1.5 nM (LT3(ITD, F691L)), 5.8 nM (FLT3-ITD), 2.3 nM (FLT3wt)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Jurkat	IC₅₀	>1 μM Compound: 4b; BSc5371	Cytotoxicity in human Jurkat cells assessed as inhibition of cellular viability after 72 hrs by CellTiter-Glo luminescent assay Cytotoxicity in human Jurkat cells assessed as inhibition of cellular viability after 72 hrs by CellTiter-Glo luminescent assay	[PMID: 30742435]
MOLM-14	IC₅₀	7.8 nM Compound: 4b; BSc5371	Cytotoxicity in human MOLM14 cells assessed as inhibition of cellular viability after 72 hrs by CellTiter-Glo luminescent assay Cytotoxicity in human MOLM14 cells assessed as inhibition of cellular viability after 72 hrs by CellTiter-Glo luminescent assay	[PMID: 30742435]
MV4-11	IC₅₀	6 nM Compound: 4b; BSc5371	Cytotoxicity in human MV4-11 cells assessed as inhibition of cellular viability incubated for 72 hrs by CellTiter-Blue assay Cytotoxicity in human MV4-11 cells assessed as inhibition of cellular viability incubated for 72 hrs by CellTiter-Blue assay	[PMID: 30742435]
THP-1	IC₅₀	>1 μM Compound: 4b; BSc5371	Cytotoxicity in human THP1 cells assessed as inhibition of cellular viability after 72 hrs by CellTiter-Glo luminescent assay Cytotoxicity in human THP1 cells assessed as inhibition of cellular viability after 72 hrs by CellTiter-Glo luminescent assay	[PMID: 30742435]

In Vitro

BSc5371 (5 μM-0.5 nM, 77 hours) exhibits inhibitory activity against FLT3-mutated (MV4-11) cells, with an IC₅₀ of 6 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Cell Viability Assay^[1]

View Protocol

Cell Line:

MV4-11 cells
Concentration:

0.5 nM, 2.5 nM, 5 nM, 25 nM, 50 nM, 250 nM, 500 nM and 5 μM
Incubation Time:

77 hours
Result:

Exhibited inhibitory activity against MV4-11 cells, with an IC₅₀ of 6 nM.

Chemical Information

CAS No. 2286419-03-0
Masse moléculaire 485.60
Formule C₂₄H₃₁N₅O₄S
SMILES

O=C(C1=C(C)NC(/C=C2C(NC3=C\2C=C(NS(=O)(C=C)=O)C=C3)=O)=C1C)NCCN(CC)CC
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocole

All Cell Biology

Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (268 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Bensinger D, et al. Virtual Screening Identifies Irreversible FMS-like Tyrosine Kinase 3 Inhibitors with Activity toward Resistance-Conferring Mutations. J Med Chem. 2019 Mar 14;62(5):2428-2446. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Keywords

MOQ

Minimum order quantity

100 mg

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