Anti-inflammatory agent 99
Anti-inflammatory agent 99 is a chalcone derivative. Anti-inflammatory agent 99 inhibits LPS (HY-D1056)-induced NF-κB nuclear translocation and suppress the phosphorylation of JNK, ERK, and p38. Anti-inflammatory agent 99 inhibits the expression of cytoinflammatory factors such as TNF-α and IL-6 induced by LPS. Anti-inflammatory agent 99 can be used for the research of LPS-induced septic shock.
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- CAS No.: 1345412-48-7
- Formule: C16H12F2O2
- Masse moléculaire:274.26
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Activité biologique
|
JNK |
NF-κB |
p38 |
ERK |
IL-6 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| RAW264.7 | IC50 |
5.55 μM
Compound: 26
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL6 production incubated for 2 hrs prior to LPS-challenge measured after 22 hrs by ELISA
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL6 production incubated for 2 hrs prior to LPS-challenge measured after 22 hrs by ELISA
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[PMID: 21988173] |
Anti-inflammatory agent 99 (Compound 26) (2.5-10 μM; 24 h) dose-dependently inhibits LPS-induced TNF-α and IL-6 release in mouse RAW 264.7 macrophages, with IC50 values of 8.42 μM and 5.55 μM, respectively[1].
Anti-inflammatory agent 99 (10 μM; 8 h) inhibits LPS-induced TNF-α, IL-6, IL-1β, and IL-12 mRNA expression in mouse primary peritoneal macrophages, but does not significantly reduce COX-2 mRNA levels[1].
Anti-inflammatory agent 99 (2.5-20 μM; 2.5-3 h) inhibits LPS-induced NF-κB p65 nuclear translocation, and dose-dependently prevents LPS-induced IκB degradation in mouse primary peritoneal macrophages[1].
Anti-inflammatory agent 99 (2.5-20 μM; 2.5 h) dose-dependently inhibits LPS-induced JNK phosphorylation in mouse primary peritoneal macrophages[1].
Anti-inflammatory agent 99 (2.5-20 μM; 3 h) inhibits LPS-induced ERK and p38 phosphorylation, and dose-dependently blocks LPS-induced p38 and ERK activation in mouse primary peritoneal macrophages[1].
Anti-inflammatory agent 99 (10 μM; 5 h) inhibits high Glucose (HY-B0389)-induced TNF-α, IL-1β, IL-6, and IL-12 mRNA expression in mouse primary peritoneal macrophages[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:mouse RAW 264.7 macrophages
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Concentration:2.5 μM; 5.0 μM; 10 μM
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Incubation Time:24 h
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Result:Dose-dependently inhibited LPS-induced TNF-α and IL-6 release.
Exhibited an IC50 of 8.42 μM for TNF-α inhibition and an IC50 of 5.55 μM for IL-6 inhibition.
Significantly reduced TNF-α and IL-6 levels compared to the LPS-only group at 10 μM (p < 0.01).
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Cell Line:mouse primary peritoneal macrophages
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Concentration:10 μM
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Incubation Time:8 h
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Result:Inhibits LPS-induced TNF-α, IL-6, IL-1β, and IL-12 mRNA expression.
Did not significantly reduce COX-2 mRNA levels.
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Cell Line:mouse primary peritoneal macrophages
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Concentration:10 μM
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Incubation Time:2.5 h
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Result:Inhibited LPS-induced NF-κB p65 nuclear translocation.
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Cell Line:mouse primary peritoneal macrophages
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Concentration:2.5 μM; 5 μM; 10 μM; 20 μM
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Incubation Time:3 h
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Result:Dose-dependently inhibited LPS-induced IκB degradation, JNK phosphorylation and activation of p38 and ERK.
Inhibited LPS-induced ERK and p38 phosphorylation at 10 μM.
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Cell Line:mouse primary peritoneal macrophages
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Concentration:10 μM
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Incubation Time:5 h
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Result:Inhibited high Glucose-induced TNF-α, IL-1β, IL-6, and IL-12 mRNA expression.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (B6) (male, 18−22 g, LPS-induced inflammatory shock model)[1]
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Dosage:15 mg/kg
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Administration:i.v.; single dose
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Result:Exhibited an 80% survival rate over the 7-day observation period.
Showed body weight decrease during the first 60 hours after LPS treatment that regained slowly afterward.
Chemical Information
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CAS No. 1345412-48-7
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Masse moléculaire 274.26
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Formule C16H12F2O2
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SMILES
O=C(C1=CC=C(C=C1)OC)/C=C/C2=C(C=CC=C2F)F
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-inflammatory agent 99 Related Classifications
HY-183934 Related Classifications
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