Calcein (solution)
Based on 1 Customer Validation
Calcein (solution) (Fluorexon (solution)) is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein cannot directly cross the intact cell membrane of a living cell, unlike Calcein-AM (HY-D0041) which is cell-permeable. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems.
Solvent and concentration: DMSO: 2 mM
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- CAS No.: 1461-15-0
- Formule: C30H26N2O13
- Masse moléculaire:622.53
-
Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
-
Activité biologique
-
Chemical Information
- Pureté et documentation
- Références
-
Help & FAQs
Activité biologique
Solvent and concentration: DMSO: 2 mM
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| CHO | Inhibition |
105.616 %
Compound: CALCEIN
|
Inhibition of sodium fluorescein uptake in OATP1B1-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM
Inhibition of sodium fluorescein uptake in OATP1B1-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM
|
[PMID: 23571415] |
| CHO | Inhibition |
121.275 %
Compound: CALCEIN
|
Inhibition of sodium fluorescein uptake in OATP1B3-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM
Inhibition of sodium fluorescein uptake in OATP1B3-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM
|
[PMID: 23571415] |
Description: Calcein is a fluorescent probe with yellow green fluorescence, it can be used to determine labile iron pool and measure the Fe(III)-binding affinity with ligands[3].
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation[3]
1.1 Preparation of working solution
Dilute to 0.5 μM with PBS or cell culture medium.
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 Cells (106/mL) are loaded by incubation with 20 μM Fe(II) for 2 h at 37°C.
2.2 Cells are collected by centrifugation (1000 rpm; 5 min) and Calcein (0.5 μM) is directly injected into the cells via microinjection for staining (5-15 mins).
2.3 Wash cells for twice by D’hanks buffer (pH 7.4).
2.4 The fluorescence is monitored by both flow cytometry and a confocal laser scanning microscope.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1461-15-0
-
Appearance Liquid (Density: 1.7±0.1 g/cm3)
-
Masse moléculaire 622.53
-
Formule C30H26N2O13
-
Color Light yellow to yellow
-
SMILES
O=C1OC2(C3=C(OC4=C2C=C(CN(CC(O)=O)CC(O)=O)C(O)=C4)C=C(O)C(CN(CC(O)=O)CC(O)=O)=C3)C5=C1C=CC=C5
-
Synonyms
Fluorexon (solution)
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
-
Fiche technique (274 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Instruction de manipulation (2659 KB)
Références
[1]. Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203. [Content Brief]
[2]. Shao B, et al. Caffeic Acid Phenyl Ester (CAPE) Protects against Iron-Mediated Cellular DNA Damage through Its Strong Iron-Binding Ability and High Lipophilicity. Antioxidants (Basel). 2021 May 18;10(5):798. [Content Brief]
[3]. Maherani B, et al., Calcein release behavior from liposomal bilayer; influence of physicochemical/mechanical/structural properties of lipids. Biochimie. 2013 Nov;95(11):2018-33. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz