COQ7-IN-1
Based on 1 Customer Validation
COQ7-IN-1, a highly potent inhibitor of human coenzyme Q (COQ7), interferes with ubiquinone (UQ) synthesis. COQ7-IN-1 does not disturb physiological cell growth of human normal culture cells. COQ7-IN-1 can be used for the research of the balance between UQ supplementation pathways: de novo UQ synthesis and extracellular UQ uptake.
For research use only. We do not sell to patients.
- Purity : 99.55%
- CAS No.: 2579696-72-1
- Formula: C14H10ClN3O
- Molecular Weight:271.70
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| C3A | GI50 |
6.1 μM
Compound: 8
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Growth inhibition of human C3A cells after 3 days in presence of 10 mM 2-DG by cell-titer-Glo assay
Growth inhibition of human C3A cells after 3 days in presence of 10 mM 2-DG by cell-titer-Glo assay
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[PMID: 31753803] |
| C3A | GI50 |
9 μM
Compound: 8
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Growth inhibition of human C3A cells assessed as growth inhibition after 3 days by cell-titer-Glo assay
Growth inhibition of human C3A cells assessed as growth inhibition after 3 days by cell-titer-Glo assay
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[PMID: 31753803] |
| HeLa | GI50 |
>12 μM
Compound: 8
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Growth inhibition of human HeLa cells assessed as growth inhibition after 3 days by cell-titer-Glo assay
Growth inhibition of human HeLa cells assessed as growth inhibition after 3 days by cell-titer-Glo assay
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[PMID: 31753803] |
| PANC-1 | GI50 |
>12 μM
Compound: 8
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Growth inhibition of human PANC1 cells after 3 days by cell-titer-Glo assay
Growth inhibition of human PANC1 cells after 3 days by cell-titer-Glo assay
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[PMID: 31753803] |
| PC-3 | GI50 |
>12 μM
Compound: 8
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Cytotoxicity against human PC3 cells assessed as growth inhibition after 3 days by cell-titer-Glo assay
Cytotoxicity against human PC3 cells assessed as growth inhibition after 3 days by cell-titer-Glo assay
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[PMID: 31753803] |
In Vitro
COQ7-IN-1 (Compound 8; 0-30 μM; for 4 days) inhibits WI-38 cells growth with a GI50 of 19.0 μM. COQ7-IN-1 weakly inhibits C3A cells growth with GI50 of 9.0±1.1 μM. COQ7-IN-1 does not limit C3A cell survival through inhibiting aerobic respiration or increasing oxidative stress[1].
COQ7 converts demethoxy-UQ (DMQ) to demethyl-UQ (DMeQ), and then converted to UQ by COQ3. Cells under COQ7 deficiency are unable to produce UQ, and consequently accumulate DMQ. DMQ10 and UQ10 contents in each culture well are 13.2 and 12.2 ng/well for COQ7-IN-1 (10 μM; for 2 days) cultured with HeLa cells. DMQ10 content of COQ7-IN-1 in HeLa cells is 52.0%[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:WI-38 cells
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Concentration:0-30 μM
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Incubation Time:4 days
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Result:The concentration that caused 50% cell growth inhibition (GI50) was 19.0 μM.
Chemical Information
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CAS No. 2579696-72-1
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Appearance Solid
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Molecular Weight 271.70
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Formula C14H10ClN3O
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Color Off-white to light yellow
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SMILES
OC1=CC(C2=CC=CC=C2)=NN1C3=CC=C(Cl)C=N3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (368.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Protocol for Cell Counting and Cell Density Analysis
Cell counting and cell-density analysis estimate the number of cells in a known volume or field area. Manual hemocytometer counting uses a chamber of defined geometry to convert counted cells into cells/mL, while automated counters and image-analysis workflows detect cell objects from optical, brightfield, fluorescence, impedance, or digital-image features. Trypan blue viability counting is based on dye exclusion: viable cells with intact membranes exclude dye, while non-viable cells with compromised membranes stain blue. The readout is total cell density, viable-cell density, dead-cell density, and percent viability. Cell density can also be estimated from microscopy images by counting objects per image area, from flow cytometry using calibrated volume or reference particles, or from in situ microscopy in bioreactors after calibration against reference methods such as hemocytometer or flow cytometry.
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Somatic Cell Culture
A method of simulating the in vivo environment in vitro to maintain the cell growth, differentation and main functions.
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CCK-8/WST-8 Cell Proliferation Assay
The CCK-8/WST-8 assay is based on the reduction of the water-soluble tetrazolium salt WST-8 to a water-soluble formazan product by cellular dehydrogenases in metabolically active cells, where the generated formazan amount is proportional to the number of living cells and is quantified by measuring absorbance in the visible range, providing a colorimetric readout for cell viability and proliferation assessment. This class of tetrazolium-based assays improves upon earlier MTT-based systems by producing a water-soluble formazan, eliminating the need for organic solubilization steps and enabling direct spectrophotometric measurement in culture medium.
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Cell Counting-Based Growth Curve Assay
Cell counting-based growth curve assays quantify cell proliferation by directly measuring changes in viable cell number over time using manual or automated counting methods such as hemocytometer-based counting or instrument-assisted cell enumeration, enabling construction of growth curves that reflect population expansion dynamics in response to culture conditions. A widely used approach is trypan blue exclusion with hemocytometer counting, where membrane-compromised (non-viable) cells take up the dye, allowing discrimination between viable and non-viable cells while simultaneously enabling total cell number quantification. Repeated sampling across time points allows estimation of proliferation rate, growth phases, and comparative growth kinetics between experimental conditions.
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MTT Cell Proliferation Assay
The MTT assay is a colorimetric endpoint assay for estimating viable cell number, cell growth, cytotoxicity, or cell activation in cultured mammalian cells. Living cells reduce the yellow tetrazolium salt MTT into purple/blue formazan, while dead cells do not generate the same signal; the resulting color can be quantified with a multiwell spectrophotometer. MTT reduction is commonly interpreted as a readout of metabolic activity that often correlates with viable cell number, but it should not be treated as a direct cell-counting method unless the assay is optimized for the cell type and experimental condition. Studies show that MTT reduction can involve mitochondrial and non-mitochondrial reducing systems, and formazan may accumulate in intracellular lipid droplets rather than simply marking mitochondria.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6805 mL | 18.4026 mL | 36.8053 mL | 92.0132 mL |
| 5 mM | 0.7361 mL | 3.6805 mL | 7.3611 mL | 18.4026 mL | |
| 10 mM | 0.3681 mL | 1.8403 mL | 3.6805 mL | 9.2013 mL | |
| 15 mM | 0.2454 mL | 1.2268 mL | 2.4537 mL | 6.1342 mL | |
| 20 mM | 0.1840 mL | 0.9201 mL | 1.8403 mL | 4.6007 mL | |
| 25 mM | 0.1472 mL | 0.7361 mL | 1.4722 mL | 3.6805 mL | |
| 30 mM | 0.1227 mL | 0.6134 mL | 1.2268 mL | 3.0671 mL | |
| 40 mM | 0.0920 mL | 0.4601 mL | 0.9201 mL | 2.3003 mL | |
| 50 mM | 0.0736 mL | 0.3681 mL | 0.7361 mL | 1.8403 mL | |
| 60 mM | 0.0613 mL | 0.3067 mL | 0.6134 mL | 1.5336 mL | |
| 80 mM | 0.0460 mL | 0.2300 mL | 0.4601 mL | 1.1502 mL | |
| 100 mM | 0.0368 mL | 0.1840 mL | 0.3681 mL | 0.9201 mL |
COQ7-IN-1 Related Classifications
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