D-myo-Inositol 1,4,5-trisphosphate sodium salt
Based on 1 publication(s) in Google Scholar
D-myo-inositol 1,4,5-trisphosphate sodium salt is a widely distributed intracellular second messenger. D-myo-inositol 1,4,5-trisphosphate sodium salt acts as a potent agonist of the Ins (1,4,5) P3 receptor with an EC50 of 0.12 μM and inhibits bovine PLC-δ1, with an IC50 of 5.4 μM for binding and an IC50 of 12.4 μM for hydrolysis. D-myo-inositol 1,4,5-trisphosphate sodium saltis metabolized into Ins (1,4) P2 and Ins (1,3,4,5) P4. D-myo-inositol 1,4,5-trisphosphate sodium salt mediates various cellular processes, including growth, development, fertilization, secretion, contraction and neuromodulation. D-myo-inositol 1,4,5-trisphosphate sodium salt inhibits PIP2 hydrolysis and Ca2+-ATPase activity. D-myo-inositol 1,4,5-trisphosphate sodium salt is used for cardiac preconditioning, exerts cardioprotective effects and mimics ischemic preconditioning. D-myo-inositol 1,4,5-trisphosphate sodium salt is applied in studies related to intracellular calcium and phosphoinositol signaling pathways.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 108340-81-4
- Formula: C6H9Na6O15P3
- Molecular Weight:551.99
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
D-myo-inositol 1,4,5-trisphosphate sodium salt acts as the natural substrate for human erythrocyte membrane Ins(1,4,5)P3 5-phosphatase with a Km of 40 μM, and potently mobilizes intracellular Ca2+ in electrically permeabilized human neuroblastoma cells with an EC50 of 0.12 μM[1].
D-myo-inositol 1,4,5-trisphosphate sodium salt acts as the natural substrate for crude rat brain Ins(1,4,5)P3 3-kinase with a Km of 0.6 μM[1].
D-myo-inositol 1,4,5-trisphosphate (15-60 μM; room temperature) sodium salt competitively inhibits the binding of PLC-δ1 to PC/PIP2 (98:2) LUVs with an IC50 of 5.4 μM[2].
D-myo-Inositol 1,4,5-trisphosphate (60 μM; 2-5 min preincubation for some experiments) sodium salt inhibits PLC-δ1-catalyzed PIP2 hydrolysis in PC/PIP2 (95:5) LUVs with an IC50 of 12.4 μM[2].
D-myo-Inositol 1,4,5-trisphosphate (2 mM MgCl2; 2 min) sodium salt is broken down by a Mg2+-dependent phosphatase in partially purified rat hepatocyte plasma membranes[3].
D-myo-Inositol 1,4,5-trisphosphate (1.5 mM free Mg2+; 10 min at 37 °C) sodium salt phosphatase activity is almost exclusively localized to the plasma membrane fraction of rat liver[3].
D-myo-Inositol 1,4,5-trisphosphate (1.0-5.0 mM calcium, manganese, putrescine, spermidine, spermine; 0.5-1.5 mM free Mg2+) sodium salt phosphatase activity in purified rat hepatocyte plasma membranes is Mg2+-dependent, with maximal activity at 0.5-1.5 mM free Mg2+[3].
D-myo-Inositol 1,4,5-trisphosphate (1.5 mM free Mg2+; 1-60 min at 37 °C) sodium salt is degraded linearly for the first 20 min by purified rat hepatocyte plasma membranes, with approximately 65% of the substrate broken down after 60 min[3].
D-myo-Inositol 1,4,5-trisphosphate sodium salt potently induces dose-dependent Ca2+ release from rat brain microsomes with an EC50 of 0.13 μM, fully mobilizing the Ins(1,4,5)P3-sensitive Ca2+ store[4].
D-myo-Inositol 1,4,5-trisphosphate sodium salt potently displaces specific [3H]Ins(1,4,5)P3 binding to rat cerebellar microsomes with an IC50 of 0.031 μM, indicating strong affinity for the Ins(1,4,5)P3 receptor[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 108340-81-4
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Appearance Solid
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Molecular Weight 551.99
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Formula C6H9Na6O15P3
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Color Light yellow to brown
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SMILES
O=P(O[Na])(O[Na])O[C@H]1[C@H](O)[C@@H](OP(O[Na])(O[Na])=O)[C@H](OP(O[Na])(O[Na])=O)[C@@H](O)[C@H]1O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Asian J Androl
Participation of the inositol 1,4,5-trisphosphate-gated calcium channel in the zona pellucida- and progesterone-induced acrosome reaction and calcium influx in human spermatozoa. [Abstract]2020 Mar-Apr;22(2):192-199. PMID: 31169139
Solvent & Solubility
In Vitro:
H2O : 2 mg/mL (3.62 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (284 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Lampe D, et al. Synthesis of selective non-Ca(2+)-mobilizing inhibitors of D-myo-inositol 1,4,5-trisphosphate 5-phosphatase. J Med Chem. 1994;37(7):907-912. [Content Brief]
[2]. Cifuentes ME, et al. D-myo-inositol 1,4,5-trisphosphate inhibits binding of phospholipase C-delta 1 to bilayer membranes. J Biol Chem. 1994;269(3):1945-1948. [Content Brief]
[3]. Seyfred MA, et al. Characterization of D-myo-inositol 1,4,5-trisphosphate phosphatase in rat liver plasma membranes. J Biol Chem. 1984;259(21):13204-13208. [Content Brief]
[4]. Lu PJ, et al. Molecular interactions of endogenous D-myo-inositol phosphates with the intracellular D-myo-inositol 1,4,5-trisphosphate recognition site. Biochemistry. 1994;33(38):11586-11597. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.8116 mL | 9.0581 mL | 18.1163 mL | 45.2907 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
D-myo-Inositol 1,4,5-trisphosphate sodium salt Related Classifications
HY-W795960 Related Classifications
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Keywords
- D-myo-Inositol 1,4,5-trisphosphate
- 108340-81-4
- Biochemical Assay Reagents
- Calcium Channel
- Phospholipase
- bovine PLC-δ1
- rat hepatocyte plasma membranes
- Ins(1,4,5)P3 receptor
- smooth muscle contraction
- rat brain
- intracellular calcium
- rat cerebellar microsomes
- Ins(1,4,5)P3 5-phosphatase
- Ins(1,4,5)P3 3-kinase
- human neuroblastoma cells
- Inhibitor
- inhibitor
- inhibit