DBCO-(PEG2-VC-PAB-MMAE)2
Based on 1 Customer Validation
DBCO-(PEG2-VC-PAB-MMAE)2 is made by MMAE conjugated to the cleavable DBCO-(PEG2-VC-PAB)2 linker. Monomethyl auristatin E (MMAE), a potent tubulin inhibitor, is a toxin payload in antibody agent conjugate. DBCO-(PEG2-VC-PAB-MMAE)2 is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity : 99.55%
- CAS No.: 2259318-55-1
- Formula: C149H224N22O32
- Molecular Weight:2835.50
-
Storage:Powder -20°C, 3 years
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
-
HY-136314
DBCO-PEG4-VC-PAB-MMAEDrug-Linker Conjugate for ADC -
HY-130990
DBCO-PEG4-Val-Cit-PAB-MMAFDrug-Linker Conjugates for ADC -
HY-133492
DBCO-PEG4-MMAFDrug-Linker Conjugate for ADC -
HY-128946
CL2A-SN-38DNA Topoisomerase I Inhibitor -
HY-112786
MC-Val-Cit-PAB-MMAFDrug-Linker Conjugate for ADC -
HY-134723
DBCO-PEG4-GGFG-DxdDrug-Linker Conjugates for ADC -
HY-148057
TCO-PEG4-VC-PAB-MMAEDrug-Linker Conjugate For ADC -
HY-141155
endo-BCN-PEG4-Val-Cit-PAB-MMAEDrug-Linker Conjugates for ADC -
HY-100874
N3-PEG3-vc-PAB-MMAEADC Conjugate -
HY-15575
VcMMAEDrug-Linker Conjugate for ADC -
HY-145934
UDP-GalNAz disodiumUDP-GalNAc Analog -
HY-147270
MC-VA-PAB-ExatecanDrug-Linker Conjugate for ADC
All Drug-Linker Conjugates for ADC Isoforms
More
Biological Activity
Description
IC50 & Target
[1]|
Dual payloads |
Auristatin |
In Vitro
ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS No. 2259318-55-1
-
Appearance Solid
-
Molecular Weight 2835.50
-
Formula C149H224N22O32
-
Color White to light yellow
-
SMILES
O=C(N1[C@](CCC1)([H])[C@H](OC)[C@@H](C)C(N[C@H](C)[C@H](C2=CC=CC=C2)O)=O)C[C@@H](OC)[C@@]([C@@H](C)CC)([H])N(C)C([C@H](C(C)C)NC([C@H](C(C)C)N(C)C(OCC(C=C3)=CC=C3NC([C@H](CCCNC(N)=O)NC([C@H](C(C)C)NC(CCOCCOCCN(CCOCCOCCC(N[C@@H](C(C)C)C(N[C@@H](CCCNC(N)=O)C(NC4=CC=C(COC(N(C)[C@@H](C(C)C)C(N[C@@H](C(C)C)C(N(C)[C@]([C@@H](C)CC)([H])[C@H](OC)CC(N5[C@](CCC5)([H])[C@H](OC)[C@@H](C)C(N[C@H](C)[C@H](C6=CC=CC=C6)O)=O)=O)=O)=O)=O)C=C4)=O)=O)=O)C(CCC(N7C8=CC=CC=C8C#CC9=CC=CC=C9C7)=O)=O)=O)=O)=O)=O)=O)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years * The compound is unstable in solutions, freshly prepared is recommended.
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (35.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
-
Data Sheet (281 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.3527 mL | 1.7634 mL | 3.5267 mL | 8.8168 mL |
| 5 mM | 0.0705 mL | 0.3527 mL | 0.7053 mL | 1.7634 mL | |
| 10 mM | 0.0353 mL | 0.1763 mL | 0.3527 mL | 0.8817 mL | |
| 15 mM | 0.0235 mL | 0.1176 mL | 0.2351 mL | 0.5878 mL | |
| 20 mM | 0.0176 mL | 0.0882 mL | 0.1763 mL | 0.4408 mL | |
| 25 mM | 0.0141 mL | 0.0705 mL | 0.1411 mL | 0.3527 mL | |
| 30 mM | 0.0118 mL | 0.0588 mL | 0.1176 mL | 0.2939 mL |
DBCO-(PEG2-VC-PAB-MMAE)2 Related Classifications
HY-126690 Related Classifications
Powered by Bioz