Art. -Nr.: HY-100737

ML349

8 Cited Publications

Kundenbewertung

Based on 8 publication(s) in Google Scholar

ML349 is a potent and specific acyl protein thioesterase 2(APT2)/lysophospholipase 2 (LYPLA2) inhibitor with a K_i of 120 nM. ML349 is also an inhibitor of LYPLA2 with an IC₅₀ of 144 nM.

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Reinheit: 98.90%
CAS. Nr.: 890819-86-0
Formel: C₂₃H₂₂N₂O₄S₂
Molecular Weight:454.56

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

ML349 Chemical Structure

CAS. Nr.: 890819-86-0

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (8)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Ki: 120 nM (APT-2)^[1]
IC50: 144 nM (LYPLA2)^[2]

Alle Phospholipase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	IC₅₀	>10 μM Compound: ML349	Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged PDXK expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged PDXK expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay	[PMID: 28197315]
HEK-293T	IC₅₀	1.1 μM Compound: ML349	Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay	[PMID: 28197315]

In Vitro

ML349 is an inhibitor of acyl protein thioesterase 1 and 2 (APT-1 and APT-2) with K_is of >10000 and 120±20 nM, respectively^[1].
ML349 is also an inhibitor of both LYPLA1 and LYPLA2 with IC₅₀s of >3000 and 144 nM, respectivley^[2].
ML348 and ML349 do not decrease cell viability, but they lead to a slight activation of AKT in NRAS mutant cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 890819-86-0
Appearance Solid
Molecular Weight 454.56
Formel C₂₃H₂₂N₂O₄S₂
Color White to yellow
SMILES

COC1=CC=C(N2CCN(C(C3=CC4=C(C(C=CC=C5)=C5S(C4)(=O)=O)S3)=O)CC2)C=C1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (8)

All WBBio/Physico-chemical AssayRT-PCRIP

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Journal Impact Factor

Nat Cancer

Scribble mis-localization induces adaptive resistance to KRAS G12C inhibitors through feedback activation of MAPK signaling mediated by YAP-induced MRAS. [Abstract]

2023 Jun;4(6):829-843. PMID: 37277529
Nat Commun

Palmitoylation of TBK1 enhances the type I interferon signaling and strengthens anti-malarial immunity in mice. [Abstract]

2025 Nov 18;16(1):10109. PMID: 41253837

ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109. [Abstract]

PEMs were treated with ML349 (0-15 μM) at the indicated concentrations for 12 h. Cell lysates were collected for ABE assay and immunoblot analysis. This was repeated n = 3 independent times with similar results.

ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109. [Abstract]

HEK293T cells were transfected with a luciferase reporter for IFN-β-luc and FLAG-TBK1 for 24 h, followed by treatment with ML349 (0-12 h) at different time points. Cell lysates were collected for luciferase reporter assays (n = 3 biological replicates).

ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109. [Abstract]

PEMs were treated with ML349 (10 μM) for 12 h, then stimulated with gDNA at the indicated time points. Cell lysates were collected for immunoblot.

ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109. [Abstract]

PEMs were treated with ML349 (10 μM) for 12 h, then stimulated with gDNA at the indicated time points. Cell lysates were collected for RT-qPCR analysis.

ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109. [Abstract]

PEMs were treated with ML349 (0-15 μM) at indicated concentrations for 12 h. Cell lysates were collected for immunoblot analysis. This was repeated n = 3 independent times with similar results.
Adv Sci (Weinh)

SMAD4 Palmitoylation Drives a Metabolic-Transcriptional Circuit to Promote Tumorigenesis and Confers Radiosensitivity in Pancreatic Cancer. [Abstract]

2026 Mar 26:e19791. PMID: 41885390
EMBO J

S-palmitoylation modulates ATG2-dependent non-vesicular lipid transport during starvation-induced autophagy. [Abstract]

2025 May;44(9):2596-2619. PMID: 40128367
Cell Rep

Targeting ZDHHC12-mediated PARP1 palmitoylation potentiates PARP inhibitor cytotoxicity. [Abstract]

2026 Jan 24;45(2):116910. PMID: 41581148
Sci Signal

SMAD2 S-palmitoylation promotes its linker region phosphorylation and TH17 cell differentiation in a mouse model of multiple sclerosis. [Abstract]

2025 May 27;18(888):eadr2008. PMID: 40424363
J Virol

Palmitoylation of CLDN12 regulated by ZDHHC7 and APT1/2 promotes hepatitis C virus entry. [Abstract]

2026 Jun 18:e0063826. PMID: 42312836
Virology

ZDHHC14 and APT2 regulate the palmitoylation of HSV-2 gB. [Abstract]

2025 Jun 25:610:110614. PMID: 40582294

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 25 mg/mL (55.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1999 mL

10.9996 mL

21.9993 mL

5 mM

0.4400 mL

2.1999 mL

4.3999 mL

10 mM

0.2200 mL

1.1000 mL

2.1999 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2 mg/mL (4.40 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Cell Assay^[3]

Cells are plated in 96-well plates with a density of 4000 to 8000 cells per well and incubated for 24 h at 37°C with 5% CO₂. Then cells are treated with increasing drug (including ML349) concentrations and their combinations. Cell viability is measured with the cell viability assay according to the manufacturer's protocol^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Won SJ, et al. Molecular Mechanism for Isoform-Selective Inhibition of Acyl Protein Thioesterases 1 and 2 (APT1 and APT2). ACS Chem Biol. 2016 Dec 16;11(12):3374-3382. [Content Brief]

[2]. Adibekian A, et al. Characterization of a Selective, Reversible Inhibitor of Lysophospholipase 2 (LYPLA2). [Content Brief]

[3]. Vujic I, et al. Acyl protein thioesterase 1 and 2 (APT-1, APT-2) inhibitors palmostatin B, ML348 and ML349 have different effects on NRAS mutant melanoma cells. Oncotarget. 2016 Feb 9;7(6):7297-306. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1999 mL	10.9996 mL	21.9993 mL	54.9982 mL
	5 mM	0.4400 mL	2.1999 mL	4.3999 mL	10.9996 mL
	10 mM	0.2200 mL	1.1000 mL	2.1999 mL	5.4998 mL
	15 mM	0.1467 mL	0.7333 mL	1.4666 mL	3.6665 mL
	20 mM	0.1100 mL	0.5500 mL	1.1000 mL	2.7499 mL
	25 mM	0.0880 mL	0.4400 mL	0.8800 mL	2.1999 mL
	30 mM	0.0733 mL	0.3667 mL	0.7333 mL	1.8333 mL
	40 mM	0.0550 mL	0.2750 mL	0.5500 mL	1.3750 mL
	50 mM	0.0440 mL	0.2200 mL	0.4400 mL	1.1000 mL

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ML349

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Lipid Metabolism Compound Library

Heterocyclic Compound Library

Metabolic Enzyme Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ML349

Alle Phospholipase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ML349

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Lipid Metabolism Compound Library

Heterocyclic Compound Library

Metabolic Enzyme Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ML349

Alle Phospholipase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ML349 Related Classifications

HY-100737 Related Classifications