m-3M3FBS
Based on 9 publication(s) in Google Scholar
m-3M3FBS is a potent phospholipase C (PLC) activator. m-3M3FBS stimulates superoxide generation in human neutrophils, upregulates intracellular calcium concentration, and stimulates inositol phosphate generation in various cell lines. m-3M3FBS induces monocytic leukemia cell apoptosis.
For research use only. We do not sell to patients.
- Purity: 99.99%
- CAS No.: 200933-14-8
- Formula: C16H16F3NO2S
- Molecular Weight:343.36
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
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Anti-Blood Cancer Compound Library
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Lipid Metabolism Compound Library
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Human Metabolite Library
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Anti-Pulmonary Fibrosis Compound Library
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Metabolic Enzyme Compound Library
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Cell Death Library
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Anti-Hematopathy Compound Library
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
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Bioactive Compound Library
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Protein Kinase Compound Library
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
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Mass Spectrometry Human Metabolite Library
HY-L215
Publications Citing Use of MedChemExpress (MCE) m-3M3FBS
More- Nat Commun. 2024 Jan 26;15(1):759. [Abstract]
- Cell Death Differ. 2026 May 12. [Abstract]
- Phytomedicine. 2023 Jul 25:116:154891. [Abstract]
- Phytother Res. 2023 Aug;37(8):3572-3582. [Abstract]
- Front Mol Neurosci. 2023 Jan 6:15:1078309. [Abstract]
- Vet Microbiol. 2025 Dec 9:313:110834. [Abstract]
- Biochem Biophys Res Commun. 2025 Sep 19:785:152676. [Abstract]
- bioRxiv. 2025 Nov 29.
- Res Sq. 2024 Jun 11.
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Bio/Physico-chemical Assay
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WB
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In Vivo Efficacy Study
All Phospholipase Isoforms
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Biological Activity
m-3M3FBS (5-50 μM) stimulates the formation of inositol phosphates in U937 cells[1].
m-3M3FBS (50 μM; 24 hours) inhibits the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes[3].
m-3M3FBS (50 μM; 24 hours) induces U937 cell apoptosis[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:U937 and THP-1 cells
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Concentration:50 μM
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Incubation Time:24 hours
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Result:Inhibited the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes.
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Cell Line:U937 cells
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Concentration:50 μM
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Incubation Time:24 hours
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Result:The apoptotic rate of m-3M3FBS-treated cells was 53.9%.
Chemical Information
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CAS No. 200933-14-8
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Appearance Solid
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Molecular Weight 343.36
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Formula C16H16F3NO2S
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Color White to off-white
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SMILES
O=S(C1=C(C)C=C(C)C=C1C)(NC2=CC=CC(C(F)(F)F)=C2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (9)
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Journal Impact Factor
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Most Recent
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Nat Commun
2024 Jan 26;15(1):759. PMID: 38272905
m-3M3FBS purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jan 26;15(1):759. [Abstract]
The effect of m-3M3FBS (30 μM) on AE2 activity was measured by Cl−-driven base transport analysis.
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Cell Death Differ
A neutrophil-intrinsic CKLF1-PKM2 axis drives glycolytic flux for de novo DAG synthesis and pro-inflammatory ROS production. [Abstract]2026 May 12. PMID: 42120940 -
Phytomedicine
Betulinaldehyde inhibits vascular remodeling by regulating the microenvironment through the PLCγ1/Ca2+/MMP9 pathway. [Abstract]2023 Jul 25:116:154891. PMID: 37229891 -
Phytother Res
Silibinin attenuated pseudo-allergic reactions and mast cell degranulation via PLCγ and PI3K/Akt signaling pathway. [Abstract]2023 Aug;37(8):3572-3582. PMID: 37115717
m-3M3FBS purchased from MedChemExpress. Usage Cited in: Phytother Res. 2023 Aug;37(8):3572-3582. [Abstract]
m-3M3FBS (20 μM) activated PLCγ in LAD2 cells.
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Front Mol Neurosci
The chronification mechanism of orofacial inflammatory pain: Facilitation by GPER1 and microglia in the rostral ventral medulla. [Abstract]2023 Jan 6:15:1078309. PMID: 36683848
m-3M3FBS purchased from MedChemExpress. Usage Cited in: Front Mol Neurosci. 2023 Jan 6:15:1078309. [Abstract]
PLC agonist m-3M3FBS (60 μM; 5 μL) significantly increased orofacial pain thresholds of rats with CFA-induced temporomandibular osteoarthritis.
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Vet Microbiol
miR-17195 promotes infectious bronchitis virus proliferation and macrophage-mediated inflammation via the PLCβ2-TAK1 axis. [Abstract]2025 Dec 9:313:110834. PMID: 41447967 -
Biochem Biophys Res Commun
Glucose transports in the ileum: mechanism, regulation and physiological role of ileal glucose absorption. [Abstract]2025 Sep 19:785:152676. PMID: 41005286 -
m-3M3FBS purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Nov 29.
Live-cell imaging micrographs of eGFP-NLRP10 (white) and mts-mCherry (red) expressing HeLa cells treated with m-3M3FBS (85 μM; 2-12 min).
m-3M3FBS purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Nov 29.
m-3M3FBS (85 μM; 15 min) caused the formation of both NLRP10 aggregates and ASC specks in HeLa cells, but only some NLRP10 aggregates recruited ASC.
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Solvent & Solubility
DMSO : 100 mg/mL (291.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (7.28 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Bae YS, et al. Identification of a compound that directly stimulates phospholipase C activity. Mol Pharmacol. 2003;63(5):1043-1050. [Content Brief]
[2]. Krjukova J, et al. Phospholipase C activator m-3M3FBS affects Ca2+ homeostasis independently of phospholipase C activation. Br J Pharmacol. 2004;143(1):3-7. [Content Brief]
[3]. Lee YN, et al. The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis. Cancer Lett. 2005;222(2):227-235. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.9124 mL | 14.5620 mL | 29.1240 mL | 72.8099 mL |
| 5 mM | 0.5825 mL | 2.9124 mL | 5.8248 mL | 14.5620 mL | |
| 10 mM | 0.2912 mL | 1.4562 mL | 2.9124 mL | 7.2810 mL | |
| 15 mM | 0.1942 mL | 0.9708 mL | 1.9416 mL | 4.8540 mL | |
| 20 mM | 0.1456 mL | 0.7281 mL | 1.4562 mL | 3.6405 mL | |
| 25 mM | 0.1165 mL | 0.5825 mL | 1.1650 mL | 2.9124 mL | |
| 30 mM | 0.0971 mL | 0.4854 mL | 0.9708 mL | 2.4270 mL | |
| 40 mM | 0.0728 mL | 0.3640 mL | 0.7281 mL | 1.8202 mL | |
| 50 mM | 0.0582 mL | 0.2912 mL | 0.5825 mL | 1.4562 mL | |
| 60 mM | 0.0485 mL | 0.2427 mL | 0.4854 mL | 1.2135 mL | |
| 80 mM | 0.0364 mL | 0.1820 mL | 0.3640 mL | 0.9101 mL | |
| 100 mM | 0.0291 mL | 0.1456 mL | 0.2912 mL | 0.7281 mL |
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