2-Bromohexadecanoic acid (Synonyms: 2-Bromopalmitic acid; 2-Bromopalmitate)

Name: 2-Bromohexadecanoic acid
Brand: MedChemExpress
SKU: HY-111770
Rating: 5.0 (20 reviews)

Cat. No.: HY-111770 Purity: 99.95%: Data Sheet
COA

SDS
Handling Instructions
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2-Bromohexadecanoic acid (2-Bromopalmitate; 2-BP) is a palmitoylation inhibitor targeting DHHC (Asp-His-His-Cys) protein palmitoyltransferase. 2-Bromohexadecanoic acid inhibits palmitoylation of GSDME-C during pyroptosis and inhibits BAK/BAX-Caspase 3-GSDME pathway-mediated pyroptosis.

For research use only. We do not sell to patients.

CAS No. : 18263-25-7

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 20 publication(s) in Google Scholar

20 Publications Citing Use of MCE 2-Bromohexadecanoic acid

Cell Proliferation/Viability Assay

Cell Imaging/Staining

: 2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 6:e07384. [Abstract]; HA cells were cultured with 10 µM 2‐BP during reoxygenation for 24 h. Western blot results showed a significant reduction in total TfR1 levels and total TfR1 palmitoylation levels.

: 2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 6:e07384. [Abstract]; Confocal microscopy images visually demonstrate the palmitoylation of TfR1 and its changes in localization on the cell membrane after treatment with OGD/Re and 2-BP (10 μM, 24 h).

: 2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 6:e07384. [Abstract]; Immunofluorescence results showed that OGD/Re treatment increased the co-localization of TfR1 and clathrin in HA cells, while 2-BP (10 μM, 24 h) decreased their co-localization.

: 2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: Theranostics. 2025 Jun 9;15(14):6665-6685. [Abstract]; A2058 and MHCC 97H cells were treated with T-T NPs, and cell viability was assessed with or without light using 2-bromohexadecanoic acid (5 µM, 3 h).

: 2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: J Biomed Sci. 2025 Feb 12;32(1):20. [Abstract]; Western blot detection of peroxisomes-related proteins under PA (400 μM) and PA (400 μM) with 2-BP (50 μM) conditions for 24 h in RSC96 cells. Tubulin was used as a loading control.

: 2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: J Biomed Sci. 2025 Feb 12;32(1):20. [Abstract]; Representative immunofluorescence staining for PMP70 (green) under PA (400 μM) and PA (400 μM) with 2-BP (50 μM) conditions for 24 h in RSC96 cells.

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Description

In Vitro

Palmitoylation promotes the release of GSDME-C from GSDME-N after cleavage, and 2-BP inhibits the palmitoylation process, while inhibiting the modification of GSDME-C increases the interaction between GSDME-C and GSDME-N. 2-BP inhibits TNFa+CHX-induced apoptosis but not total cell death^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

335.32

Formula

C₁₆H₃₁BrO₂

CAS No.

18263-25-7

Appearance

Solid

Color

White to yellow

SMILES

CCCCCCCCCCCCCCC(Br)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 125 mg/mL (372.78 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9822 mL	14.9111 mL	29.8223 mL
5 mM	0.5964 mL	2.9822 mL	5.9645 mL
10 mM	0.2982 mL	1.4911 mL	2.9822 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 5 mg/mL (14.91 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (267 KB) SDS (393 KB)

COA (239 KB) HNMR (251 KB) RP-HPLC (240 KB) MS (84 KB)

Handling Instructions (2659 KB)

References

[1]. Hu L, et al. Chemotherapy-induced pyroptosis is mediated by BAK/BAX-caspase-3-GSDME pathway and inhibited by 2-bromopalmitate. Cell Death Dis. 2020 Apr 24;11(4):281. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9822 mL	14.9111 mL	29.8223 mL	74.5556 mL
	5 mM	0.5964 mL	2.9822 mL	5.9645 mL	14.9111 mL
	10 mM	0.2982 mL	1.4911 mL	2.9822 mL	7.4556 mL
	15 mM	0.1988 mL	0.9941 mL	1.9882 mL	4.9704 mL
	20 mM	0.1491 mL	0.7456 mL	1.4911 mL	3.7278 mL
	25 mM	0.1193 mL	0.5964 mL	1.1929 mL	2.9822 mL
	30 mM	0.0994 mL	0.4970 mL	0.9941 mL	2.4852 mL
	40 mM	0.0746 mL	0.3728 mL	0.7456 mL	1.8639 mL
	50 mM	0.0596 mL	0.2982 mL	0.5964 mL	1.4911 mL
	60 mM	0.0497 mL	0.2485 mL	0.4970 mL	1.2426 mL
	80 mM	0.0373 mL	0.1864 mL	0.3728 mL	0.9319 mL
	100 mM	0.0298 mL	0.1491 mL	0.2982 mL	0.7456 mL

2-Bromohexadecanoic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-Bromohexadecanoic acid18263-25-72-Bromopalmitic acid 2-BromopalmitatePyroptosisInhibitorinhibitorinhibit

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2-Bromohexadecanoic acid (Synonyms: 2-Bromopalmitic acid; 2-Bromopalmitate)

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2-Bromohexadecanoic acid Related Antibodies

20 Publications Citing Use of MCE 2-Bromohexadecanoic acid

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Complete Stock Solution Preparation Table

2-Bromohexadecanoic acid Related Classifications

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