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AACOCF3  (Synonyms: Arachidonyl trifluoromethyl ketone)

Cat. No.: HY-108611 Purity: 99.89%
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AACOCF3 (Arachidonyl trifluoromethyl ketone) is a cell-permeant trifluoromethyl ketone analog of arachidonic acid. AACOCF3 is a potent and selective slow binding inhibitor of the 85-kDa cytosolic phospholipase A2 (cPLA2). AACOCF3 blocks production of arachidonate and 12-hydroxyeicosatetraenoic acid by calcium ionophore-challenged platelets. AACOCF3 inhibits glucose-induced insulin secretion from isolated rat islets. AACOCF3 has the potential for the research of cardiovascular disease.

For research use only. We do not sell to patients.

CAS No. : 149301-79-1

Size Price Stock Quantity
Solvent
5 mg (28.05 mM * 500 μL in Ethanol) In-stock
Solvent
10 mg (28.05 mM * 1 mL in Ethanol) In-stock

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Customer Review

Based on 4 publication(s) in Google Scholar

AACOCF3 Related Antibodies

Top Publications Citing Use of Products

    AACOCF3 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2025 Dec;40(1):2547259.  [Abstract]

    AACOCF3 (2 μM) significantly decreased the protein expression levels of 12/15-LOX, cPLA2, p-p38 MAPK/p38 MAPK, and TNF-α in OE-treated HT22 cells.

    AACOCF3 purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Feb:200:107082.  [Abstract]

    IC50 of AACOCF3 (1-100 μM) in MHCC97H and PLC/PRF/5.

    AACOCF3 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jul:129:155648.

    AACOCF3 (20 μM) significantly decreased the activity and content of cPLA2, AA, IL-1β, and TNF-α in NRK52E cells compared to those in the BRB group.

    AACOCF3 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jul:129:155648.

    AACOCF3 (20 μM) attenuated the BRB-induced increases in the P-cPLA2/cPLA2 ratio and the levels of caspase-1 and caspase-3 in NRK52E cells.

    AACOCF3 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Mar:116:109637.  [Abstract]

    AACOCF3 (1 μM; 4 h) decreased cPLA2 activity in LPS-treated MHS cells.

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    Description

    AACOCF3 (Arachidonyl trifluoromethyl ketone) is a cell-permeant trifluoromethyl ketone analog of arachidonic acid. AACOCF3 is a potent and selective slow binding inhibitor of the 85-kDa cytosolic phospholipase A2 (cPLA2). AACOCF3 blocks production of arachidonate and 12-hydroxyeicosatetraenoic acid by calcium ionophore-challenged platelets. AACOCF3 inhibits glucose-induced insulin secretion from isolated rat islets. AACOCF3 has the potential for the research of cardiovascular disease[1][2][3].

    Cellular Effect
    Cell Line Type Value Description References
    HL-60 IC50
    29 μM
    Compound: 1
    Inhibition of [3H]arachidonic acid production by stimulated HL60 cells.
    Inhibition of [3H]arachidonic acid production by stimulated HL60 cells.
    		[PMID: 11882004]
    THP-1 IC50
    41000 nM
    Compound: AACOCF3
    Concentration required to inhibit production of LTC4 from THP-1 cells stimulated with A-23187
    Concentration required to inhibit production of LTC4 from THP-1 cells stimulated with A-23187
    		[PMID: 11229777]
    THP-1 IC50
    86000 nM
    Compound: AACOCF3
    Concentration required to inhibit production of arachidonic acid from THP-1 cells stimulated with A-23187
    Concentration required to inhibit production of arachidonic acid from THP-1 cells stimulated with A-23187
    		[PMID: 11229777]
    In Vitro

    AACOCF3 inhibits the release of arachidonic acid from calcium ionophore-challenged U937 cells (IC50= 8 μM, 2 x 106 cells ml-1) and from platelets (IC50= 2 μM, 4 x 107 cells ml-1)[1].
    AACOCF3 (10 μM) suppresses phosphate-induced calcification and osteogenic/chondrogenic signaling in HAoSMCs. AACOCF3 significantly inhibits both basal and Pi-induced release of arachidonic acid, the product of PLA2 activity[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    AACOCF3 Related Antibodies
    In Vivo

    AACOCF3 (10 mg/kg; gavage; 5 days a week; ApoE–/– mice (6-week-old males) were fed a high-cholesterol diet) significantly reduces type III collagen plaque expression but had no significant influence on total collagen accumulation[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    356.47

    Formula

    C21H31F3O
    CAS No.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(C(F)(F)F)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Solution, -20°C, 2 years
    Purity & Documentation

    Purity: 99.89%

    SDS (393 KB)

    COA (237 KB) HNMR (256 KB) LCMS (92 KB)

    Handling Instructions (2659 KB)
    References
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    AACOCF3 Related Classifications

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    AACOCF3149301-79-1Arachidonyl trifluoromethyl ketoneAACOCF 3AACOCF-3Phospholipasevascularcalcificationglucose-inducedinsulinsecretionselectiveInhibitorinhibitorinhibit

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