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Cat. No.: HY-108611

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AACOCF3

(Synonyms: Arachidonyl trifluoromethyl ketone)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

AACOCF3 (Arachidonyl trifluoromethyl ketone) is a cell-permeant trifluoromethyl ketone analog of arachidonic acid. AACOCF3 is a potent and selective slow binding inhibitor of the 85-kDa cytosolic phospholipase A2 (cPLA2). AACOCF3 blocks production of arachidonate and 12-hydroxyeicosatetraenoic acid by calcium ionophore-challenged platelets. AACOCF3 inhibits glucose-induced insulin secretion from isolated rat islets. AACOCF3 has the potential for the research of cardiovascular disease.

For research use only. We do not sell to patients.

Purity: 99.89%
CAS No.: 149301-79-1
Formula: C₂₁H₃₁F₃O
Molecular Weight:356.47

Storage:

Solution, -20°C, 2 years

AACOCF3 Chemical Structure

CAS No.: 149301-79-1

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Phospholipase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	29 μM Compound: 1	Inhibition of [3H]arachidonic acid production by stimulated HL60 cells. Inhibition of [3H]arachidonic acid production by stimulated HL60 cells.	[PMID: 11882004]
THP-1	IC₅₀	41 μM Compound: AACOCF3	Concentration required to inhibit production of LTC4 from THP-1 cells stimulated with A-23187 Concentration required to inhibit production of LTC4 from THP-1 cells stimulated with A-23187	[PMID: 11229777]
THP-1	IC₅₀	86 μM Compound: AACOCF3	Concentration required to inhibit production of arachidonic acid from THP-1 cells stimulated with A-23187 Concentration required to inhibit production of arachidonic acid from THP-1 cells stimulated with A-23187	[PMID: 11229777]

In Vitro

AACOCF3 inhibits the release of arachidonic acid from calcium ionophore-challenged U937 cells (IC₅₀= 8 μM, 2 x 10⁶ cells ml^-1) and from platelets (IC₅₀= 2 μM, 4 x 10⁷ cells ml^-1)^[1].
AACOCF3 (10 μM) suppresses phosphate-induced calcification and osteogenic/chondrogenic signaling in HAoSMCs. AACOCF3 significantly inhibits both basal and Pi-induced release of arachidonic acid, the product of PLA2 activity^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

AACOCF3 (10 mg/kg; gavage; 5 days a week; ApoE^–/– mice (6-week-old males) were fed a high-cholesterol diet) significantly reduces type III collagen plaque expression but had no significant influence on total collagen accumulation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 149301-79-1
Appearance Liquid
Molecular Weight 356.47
Formula C₂₁H₃₁F₃O
Color Colorless to light yellow
SMILES

CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(C(F)(F)F)=O
Synonyms

Arachidonyl trifluoromethyl ketone
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Solution, -20°C, 2 years

Publications (6)

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Journal Impact Factor

Autophagy

E-cigarette aerosols induce the hydrolysis of lysosomal glycerophospholipids through PLA2G4A activation initiated by nicotine binding to CHRNA3/α3 nAChR in airway epithelial cells. [Abstract]

2026 Jun 21:1-25. PMID: 42287088
Pharmacol Res

Identification of Fasudil as a collaborator to promote the anti-tumor effect of lenvatinib in hepatocellular carcinoma by inhibiting GLI2-mediated hedgehog signaling pathway. [Abstract]

2024 Feb:200:107082. PMID: 38280440

AACOCF3 purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Feb:200:107082. [Abstract]

IC₅₀ of AACOCF3 (1-100 μM) in MHCC97H and PLC/PRF/5.
Phytomedicine

Unraveling the enigma: Molecular mechanisms of berberrubine-induced nephrotoxicity reversed by its parent form berberine. [Abstract]

2024 Jul:129:155648. PMID: 38669970

AACOCF3 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jul:129:155648. [Abstract]

AACOCF3 (20 μM) significantly decreased the activity and content of cPLA2, AA, IL-1β, and TNF-α in NRK52E cells compared to those in the BRB group.

AACOCF3 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jul:129:155648. [Abstract]

AACOCF3 (20 μM) attenuated the BRB-induced increases in the P-cPLA2/cPLA2 ratio and the levels of caspase-1 and caspase-3 in NRK52E cells.
J Ethnopharmacol

Exploring the mechanisms of Gualou Xiebai Banxia decoction against myocardial ischemia by integrating network pharmacology, metabolomics, lipidomics, and experimental validation. [Abstract]

2026 Oct 28:369:121879. PMID: 42150656
Int Immunopharmacol

Fexofenadine protects against lipopolysaccharide-induced acute lung injury by targeting cytosolic phospholipase A2. [Abstract]

2023 Mar:116:109637. PMID: 36764283

AACOCF3 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Mar:116:109637. [Abstract]

AACOCF3 (1 μM; 4 h) decreased cPLA2 activity in LPS-treated MHS cells.
J Enzyme Inhib Med Chem

Inhibition of 12/15-LOX hyperactivation mitigates cognitive decline in a chronic cerebral hypoperfusion mouse model and in H2O2-induced HT22 cells: therapeutic effects of brozopine. [Abstract]

2025 Dec;40(1):2547259. PMID: 40841277

AACOCF3 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2025 Dec;40(1):2547259. [Abstract]

AACOCF3 (2 μM) significantly decreased the protein expression levels of 12/15-LOX, cPLA2, p-p38 MAPK/p38 MAPK, and TNF-α in OE-treated HT22 cells.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (265 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Riendeau D, et al. Arachidonyl trifluoromethyl ketone, a potent inhibitor of 85-kDa phospholipase A2, blocks production of arachidonate and 12-hydroxyeicosatetraenoic acid by calcium ionophore-challenged platelets. J Biol Chem. 1994;269(22):15619-15624. [Content Brief]

[2]. Schanstra JP, et al. Systems biology identifies cytosolic PLA2 as a target in vascular calcification treatment. JCI Insight. 2019;4(10):e125638. Published 2019 May 16. [Content Brief]

[3]. Loweth AC, et al. A specific inhibitor of cytosolic phospholipase A2 activity, AACOCF3, inhibits glucose-induced insulin secretion from isolated rat islets. Biochem Biophys Res Commun. 1996;218(2):423-427. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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Amount: USD 0.00

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Purity & Documentation
References

1/16
1/16

AACOCF3 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2025 Dec;40(1):2547259. [Abstract]

Customer Validation
AACOCF3 (2 μM) significantly decreased the protein expression levels of 12/15-LOX, cPLA2, p-p38 MAPK/p38 MAPK, and TNF-α in OE-treated HT22 cells.
1/16

AACOCF3 purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Feb:200:107082. [Abstract]

Customer Validation
IC₅₀ of AACOCF3 (1-100 μM) in MHCC97H and PLC/PRF/5.
1/16

AACOCF3 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jul:129:155648. [Abstract]

Customer Validation
AACOCF3 (20 μM) significantly decreased the activity and content of cPLA2, AA, IL-1β, and TNF-α in NRK52E cells compared to those in the BRB group.
1/16

AACOCF3 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jul:129:155648. [Abstract]

Customer Validation
AACOCF3 (20 μM) attenuated the BRB-induced increases in the P-cPLA2/cPLA2 ratio and the levels of caspase-1 and caspase-3 in NRK52E cells.
1/16

AACOCF3 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Mar:116:109637. [Abstract]

Customer Validation
AACOCF3 (1 μM; 4 h) decreased cPLA2 activity in LPS-treated MHS cells.

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AACOCF3

Publications Citing Use of MedChemExpress (MCE) AACOCF3

All Phospholipase Isoforms