Demethyltorosaflavone C
Demethyltorosaflavone C is a Free Radical Scavenger, with DPPH• scavenging IC50 values of 47.7 μM and 1.36 mM in different experimental systems. The catechol group on the B ring of Demethyltorosaflavone C promotes hydrogen donation and resonance stabilization of the phenoxyl radical, thereby exerting its free radical scavenging effect. Demethyltorosaflavone C can be used in studies on oxidative stress and natural product flavonoids.
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- CAS No.: 145194-15-6
- Formula: C21H14O8
- Molecular Weight:394.33
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
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DPPH 1.36 mM (IC50) |
DPPH 0.72 mM (IC50) |
DPPH 47.7 μM (IC50) |
In Vitro
Demethyltorosaflavone C (compound 2) (30 min) scavenges DPPH• radicals in a cell-free system with an IC50 of 1.36 mM[2].
Demethyltorosaflavone C (30 min) exhibits concentration-dependent ABTS•+ radical scavenging activity with an IC50 of 0.72 mM[2].
Demethyltorosaflavone C (20 min) scavenges DPPH radicals in cell-free assays with an IC50 of 47.7 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 145194-15-6
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Molecular Weight 394.33
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Formula C21H14O8
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SMILES
CC([C@@]1([H])C2=C(O)C(C(C=C(C3=CC(O)=C(C=C3)O)O4)=O)=C4C=C2O[C@]15[H])=C(O)C5=O
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Demethyltorosaflavone C Related Classifications
HY-N21616 Related Classifications
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