UB-221
Based on 1 Customer Validation
UB-221 is an anti-IgE monoclonal antibody (human IgE Kd = 5.85×10-11 M). UB-221 blocks the IgE-FcεRI interaction and promotes CD23 binding by binding to free IgE and CD23-bound IgE, inhibits basophil activation and degranulation, stabilizes membrane-bound CD23 on B lymphocytes and downregulates IgE synthesis, and induces apoptosis (Apoptosis) of B cells expressing membrane-bound IgE. UB-221 directs IgE toward non-inflammatory clearance pathways, reduces IgE levels and FcεRI expression, and can be used for research on chronic spontaneous urticaria and food allergy.
For research use only. We do not sell to patients.
- Purity : 98.95%
- CAS No.: 2755114-30-6
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Species Reactivity
Human
IC50 & Target
IGHE
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| Ramos | EC50 |
7.9 ng/mL
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Binding to Ramos B cells expressing long-form membrane-bound IgE measured as mean EC50.
Binding to Ramos B cells expressing long-form membrane-bound IgE measured as mean EC50.
|
35912861 |
In Vitro
UB-221 does not trigger ADCC/CDC in IgE-preloaded CD23-overexpressing SKW6.4 cells and does not induce cytokine release from human PBMCs[1].
UB-221 binds IgE that is bound to CD23, and the preformed UB-221-IgE complex binds CD23 in ELISA, with EC50 values of 39.1 and 41.6 ng/mL, respectively[1].
UB-221 blocks FcεRI-mediated RBL SX-38 degranulation with an IC50 of 0.14 μg/mL and does not activate human basophils[1].
UB-221 (0-75,000 ng/mL; 1 hour at room temperature) neutralizes high IgE in human serum samples, with an EC90 of 1,097 ng/mL in the low IgE group and 27,539 ng/mL in the high IgE group[1].
UB-221 inhibits FcεRI interaction and promotes CD23 binding in in vitro IgE receptor interaction assays[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Parmacokinetics
| Species | Dose | Route | T1/2 |
|---|---|---|---|
| Cynomolgus Monkey[1] | 5.0 mg/kg | i.v. | 6.3 day |
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Cynomolgus macaque (cynomolgus monkey; n=3; suitable pharmacology and toxicology animal model)[1]
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Dosage:5.0 mg/kg
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Administration:i.v.; single dose
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Result:Induced a rapid, pronounced reduction in serum free cIgE by 90% to 100%.
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Animal Model:hIGHE-knockin (human IgE ε,κ-knockin; F1 hybrid of male C57BL/6 background crossed with female BALB/c; 8-16 weeks old)[1]
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Dosage:0.3 mg/kg or 3.0 mg/kg
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Administration:i.p.; single dose
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Result:Rapidly reduced high serum free-chimeric-IgE levels by greater than 90%.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | 2011-05 | PHASE1 |
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
ELISA, FACS, Functional assay
Chemical Information
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CAS No. 2755114-30-6
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
N/A
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
UB-221 Related Classifications
HY-P991592 Related Classifications
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