DNA gyrase/Topo IV-IN-3 

DNA gyrase/Topo IV-IN-3 is an inhibitor of Escherichia coli DNA gyrase and Topoisomerase IV, with IC₅₀ values of 1.75 μM and 3.47 μM, respectively. DNA gyrase/Topo IV-IN-3 acts as a biofilm inhibitor and exhibits antibacterial activity against both Gram-negative and Gram-positive bacteria. DNA gyrase/Topo IV-IN-3 can be used in studies related to bacterial infections^[1].

DNA gyrase/Topo IV-IN-3 (Compound 6g) exhibits strong antibacterial activity against E. coli, moderate activity against P. aeruginosa and S. aureus, and negligible activity against B. subtilis in the disk diffusion assay^[1].
DNA gyrase/Topo IV-IN-3 exhibits inhibitory activity against the growth of E. coli comparable to that of Ciprofloxacin (HY-B0356), while its inhibitory activity against P. aeruginosa and S. aureus is half that of Ciprofloxacin, with MIC values of 0.025, 0.025 and 0.125 μg/mL, respectively^[1].
DNA gyrase/Topo IV-IN-3 (50 μM; 4 days) exhibits no cytotoxicity against the normal human mammary epithelial cell line MCF-10A^[1].
DNA gyrase/Topo IV-IN-3 (0.00625-0.025 μg/mL) exhibits significant dose-dependent antibiofilm activity against E. coli, with an inhibition rate of 96% at its MIC of 0.025 μg/mL^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

693.12

C₃₇H₃₀ClFN₆O₅

OC(C1=CN(C2CC2)C3=CC(N4CCN(CC(NC5=CC=C(C(N6)=CC(C7=CC=CC(Cl)=C7)=C(C#N)C6=O)C=C5)=O)CC4)=C(F)C=C3C1=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Al-Wahaibi LH, et al. Design, synthesis, and antibacterial assessment of a new series of ciprofloxacin-based compounds as possible dual DNA gyrase/topoisomerase IV inhibitors. Sci Rep. 2026;16(1):13911. Published 2026 Apr 30.