Fluo-3 pentaammonium
Fluo-3 pentaammonium is a cell-impermeable calcium ion (Ca2+) indicator. Fluo-3 pentaammonium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm).
For research use only. We do not sell to patients.
- CAS No.: 339221-91-9
- Formula: C36H45Cl2N7O13
- Molecular Weight:854.69
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
Fluo-3 can be introduced into cells using methods such as patch clamping and microinjection.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 339221-91-9
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Molecular Weight 854.69
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Formula C36H45Cl2N7O13
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SMILES
O=C(O)CN(C1=CC=C(C)C=C1OCCOC2=CC(C3=C4C=C(Cl)C(C=C4OC5=C3C=C(Cl)C(O)=C5)=O)=CC=C2N(CC(O)=O)CC(O)=O)CC(O)=O.N.N.N.N.N
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Fluo-3 pentaammonium Related Classifications
HY-126821A Related Classifications
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