2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK
  3. EGFR
  4. H2Mab-139

H2Mab-139  (Synonyms: H2Mab-141, H2Mab-19)

Cat. No.: HY-P991980
Technical Support

H2Mab-139 (H2Mab-141;H2Mab-19) is an anti-HER2 monoclonal antibody. H2Mab-139 specifically binds to HER2 on various cancer cells. H2Mab-139 can detect HER2 overexpression in colorectal cancer via flow cytometry and immunohistochemical analysis. H2Mab-139 exhibits anti-tumor activity in mouse oral cancer and breast cancer xenografts. H2Mab-139 is applicable to research related to multiple cancers including colon cancer, oral cancer, and breast cancer.

For research use only. We do not sell to patients.

H2Mab-139

H2Mab-139 Chemical Structure

Size Stock
1 mg   Get quote  
5 mg   Get quote  
10 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

H2Mab-139 Related Antibodies

Top Publications Citing Use of Products

View All EGFR Isoform Specific Products:

View All Isoforms
EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

H2Mab-139 (H2Mab-141;H2Mab-19) is an anti-HER2 monoclonal antibody. H2Mab-139 specifically binds to HER2 on various cancer cells. H2Mab-139 can detect HER2 overexpression in colorectal cancer via flow cytometry and immunohistochemical analysis. H2Mab-139 exhibits anti-tumor activity in mouse oral cancer and breast cancer xenografts. H2Mab-139 is applicable to research related to multiple cancers including colon cancer, oral cancer, and breast cancer[1][2].

Species Reactivity

Human
In Vitro

H2Mab-139 (10 μg/mL; 30 min at 4°C) specifically binds to HER2 on LN229/HER2 glioblastoma cells and the colorectal cancer cell lines Caco-2, HCT-116, HCT-15, HT-29, LS 174T, COLO 201, COLO 205, HCT-8, SW1116, and DLD-1 in flow cytometry assays[1].
H2Mab-139 (10 μg/mL) specifically detects HER2 protein with a molecular weight of approximately 200 kDa in LN229/HER2 glioblastoma cell lysates via Western blotting[1].
H2Mab-139 (10 μg/mL; 1 h at room temperature) specifically stains the cell membrane of HER2-positive human colon cancer tissue sections in immunohistochemical assays[1].
H2Mab-19 (6 ng/mL to 100 μg/mL) maintains high binding affinity for HER2 in HER2-expressing BT-474, HSC-2, and SAS cell lines, with Kd values of 2.3 × 10-8 M, 9.5 × 10-9 M, and 5.5 × 10-9 M, respectively[2].
H2Mab-19 (5 h at 37°C) exhibits high CDC activity against BT-474, HSC-2 and SAS cell lines, with cytotoxicity rates of 46%, 51% and 53%, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

H2Mab-139 Related Antibodies
In Vivo

H2Mab-19 (100 μg per dose; i.p.; on days 1, 7, and 14 after cell implantation) significantly reduces tumor development and tumor weight in BT-474 breast cancer xenografts[2].
H2Mab-19 (100 μg per dose; i.p.; on days 1, 6, and 14 after cell implantation) significantly reduces tumor development and tumor weight in HSC-2 oral cancer xenografts[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude (female, 10 weeks old, purchased at 6 weeks old) BT-474 cells (1.33x108) [2]
Dosage: 100 μg per dose
Administration: i.p.; on days 1, 7, and 14 after cell implantation
Result: Significantly reduced tumor development compared to controls on days 5, 7, 12, 15, and 18 post-implantation.
Significantly reduced tumor weights compared to control mice.
Showed no significant difference in total body weight compared to control mice.
Animal Model: BALB/c nude (female, 10 weeks old, purchased at 6 weeks old) HSC-2 or SAS cell (1.33x108)[2]
Dosage: 100 μg per dose
Administration: i.p.; on days 1, 6, and 14 after cell implantation
Result: Significantly reduced tumor development compared to controls on days 6, 10, 14, 17, and 20 post-implantation.
Significantly reduced tumor weights compared to control mice.
Showed no significant difference in total body weight compared to control mice.\nSignificantly reduced tumor development compared to controls on days 14, 17, and 20 post-implantation.
Significantly reduced tumor weights compared to control mice.
Showed no significant difference in total body weight compared to control mice.
Gene ID

2064  [NCBI]

Accession
Target

ERBB2/HER2/CD340
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
SMILES

[H2Mab-139]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

H2Mab-139 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

H2Mab-139H2Mab-141, H2Mab-19EGFREpidermal growth factor receptorErbB-1HER1anti-HER2 monoclonal antibodyLN229/HER2 cellsCaco-2 cellsHCT-116 cellsHCT-15 cellsHT-29 cellsLS 174T cellsCOLO 201 cellsCOLO205 cellsHCT-8 cellsSW1116 cellsDLD-1 cellscolon canceroral cancerand breast cancer
Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
H2Mab-139
Cat. No.:
HY-P991980
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.