Isocucurbitacin B
Based on 1 Customer Validation
Isocucurbitacin B is a natural terpenoid compound found in Pedicellus Melo. Isocucurbitacin B can inhibit the PI3K/AKT, MAPK, and STAT3 signaling pathways and downregulate CAV1 expression. Isocucurbitacin B can inhbit cancer cell proliferation, migration and invision. Isocucurbitacin B can induce apoptosis and cause G2/M phase arrest. Isocucurbitacin B can decrease intracellular cholesterol and PH levels and increase intracellular calcium levels. Isocucurbitacin B can be used for the research of cancer, such as glioma[1][2].
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.98%
- CAS 番号: 17278-28-3
- 分子式: C32H46O8
- 分子量:558.70
-
保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
製品説明
IC50 & Target
[1]|
STAT3 |
体外実験
Isocucurbitacin B (0.001-25 μmol/L; 12-24 h) inhibits the proliferation of U251 and U87 cells[1][2].
Isocucurbitacin B (0-1 μmol/L; 24 h) induces U251 and GL261 cell apoptosis[1][2].
IsoCuB (0-1 μmol/L; 24 hours) blocks the cell cycle at the G2/M phase, reducing cyclin A2 and CDK1 expression in U251 cells[2].
Isocucurbitacin B (0-0.1 μmol/L; 12-36 hours) inhibits U251 and GL261 cells migration and invasion, reducing MMP2, N-cadherin, and vimentin levels[1][2].
Isocucurbitacin B (0-0.1 μmol/L; 24 h) inhibits U251 and GL261 cells invasion[1][2].
Isocucurbitacin B (0-1 μmol/L; 24 h) inhibits the activation of PI3K/AKT, MAPK, and STAT3 signaling pathways in U251 cells[1].
Isocucurbitacin B (0-1 μmol/L; 24 h) downregulates pdk1, RXRα, PPAα, and Bcl-2 mRNA expressions in U251 cells[1].
Isocucurbitacin B (0-1 μmol/L; 24 hours) decreases intracellular cholesterol and PH levels and increases intracellular calcium levels in U251 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:U251 and U87 cells
-
Concentration:0.001, 0.01, 0.1, 0.5, 1, 5, 25 μmol/L
-
Incubation Time:12 h, 24 h
-
Result:Inhibited U251 and U87 cell proliferation in a concentration- and time-dependent manner.
-
Cell Line:U251 and GL261 cells
-
Concentration:0, 0.1, 0.5, 1 μmol/L
-
Incubation Time:24 h
-
Result:Promoted the apoptosis of U251 and GL261 cells in a concentration-dependent manner. Increaseed BCL-2 amd BAX levels.
-
Cell Line:U251 and GL261
-
Concentration:0, 0.001, 0.01, 0.005, 0.1 μmol/L
-
Incubation Time:12 h, 24 h, 36 h
-
Result:Inhibited the migration of U251 and GL 261 cells in a concentration- and time-dependent manner.
-
Cell Line:U251 and GL261
-
Concentration:0, 0.001, 0.01, 0.05, 0.1 μmol/L
-
Incubation Time:24 h
-
Result:Inhibited the invasion of U251 and GL261 cells in a concentration-dependent manner.
-
Cell Line:U251
-
Concentration:0, 0.1, 0.5, 1 μmol/L
-
Incubation Time:24 h
-
Result:Inhibited the activation of the PI3K/AKT, MAPK, and STAT3 signaling pathways by decreasing the levels of p-MAPK1/3, total PI3K, t-AKT, PDK1, and p-STAT3 proteins.
-
Cell Line:U251
-
Concentration:0, 0.1, 0.5, 1 μmol/L
-
Incubation Time:24 h
-
Result:Downregulated the mRNA expressions of PDK1, RXRα, PPARα, and Bcl-2 in U251 cells.
-
Cell Line:U251 cells
-
Concentration:0, 0.1, 0.5, 1 μmol/L
-
Incubation Time:24 hours
-
Result:Blocked the cell cycle of U251 cells at the G2/M phase in a concentration-dependent manner and reduced expression levels of cyclin A2 and CDK1.
体内実験
Isocucurbitacin B (0.025-0.05 mg/L; 72 hours) reduces tumor burden in glioma cell-induced zebrafish[2].
Isocucurbitacin B (1 mg/kg; intraperitoneal; daily; 21 days from day 7) inhibits glioma growth in GL261 cell-induced glioma in C57BL/6 mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Six-week-old specific pathogen-free (SPF) BALB/c nude mice transplanted with U251 cells [1]
-
Dosage:2 mg/kg
-
Administration:Intraperitoneally injection; daily; 18 days from day 6
-
Result:Tumor growth was significantly inhibited. No significant effect on body weight was observed.
-
Animal Model:C57BL/6 (Male, 5 weeks old)[2]
-
Dosage:1 mg/kg
-
Administration:intraperitoneal; daily; 27 days
-
Result:Tumor volume was significantly reduced, and cell proliferation and glial fibrillary acidic protein expression were decreased.
-
Animal Model:Glioma cell-induced Zebrafish[2]
-
Dosage:0.025 mg/L, 0.05 mg/L
-
Administration:In water; 72 hours
-
Result:Fluorescence intensity was significantly reduced, indicating a reduction in tumor burden.
- RNA extraction experimental
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Cell migration
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Ca2+ Staining Technique
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- PCNA Immunodetection Proliferation Assay
- Flow cytometric DNA-content cell-cycle staining
- Protocol for Cell Counting and Cell Density Analysis
- Apoptosis Solutions
- Calcium Spark Assay
- MTT Cell Proliferation Assay
化学情報
-
CAS 番号 17278-28-3
-
性状 Solid
-
分子量 558.70
-
分子式 C32H46O8
-
Color White to off-white
-
SMILES
C[C@]12[C@@]([C@]([C@@](C)(O)C(/C=C/C(C)(C)OC(C)=O)=O)([H])[C@H](O)C1)(CC([C@]3(C)[C@@]2([H])CC=C4[C@@]3([H])CC([C@@H](O)C4(C)C)=O)=O)C
-
Structure Classification
-
Initial Source
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
純度とドキュメンテーション
-
データシート (280 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Han M, et al. Isocucurbitacin B inhibits glioma growth through PI3K/AKT pathways and increases glioma sensitivity to TMZ by inhibiting hsa-mir-1286a. Cancer Drug Resist. 2024;7:16. Published 2024 May 8. [Content Brief]
[2]. Han M, et al. Isocucurbitacin B inhibits gliomas through the promotion of anoikis by targeting caveolin 1. Cancer Lett. 2025;629:217873. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Isocucurbitacin B 関連分類
HY-N4189 関連分類
Powered by Bioz