2. PI3K/Akt/mTOR MAPK/ERK Pathway JAK/STAT Signaling Stem Cell/Wnt Apoptosis
  3. PI3K Akt p38 MAPK STAT Apoptosis
  4. Isocucurbitacin B

Isocucurbitacin B is a natural terpenoid compound found in Pedicellus Melo. Isocucurbitacin B can inhibit the PI3K/AKT, MAPK, and STAT3 signaling pathways and downregulate CAV1 expression. Isocucurbitacin B can inhbit cancer cell proliferation, migration and invision. Isocucurbitacin B can induce apoptosis and cause G2/M phase arrest. Isocucurbitacin B can decrease intracellular cholesterol and PH levels and increase intracellular calcium levels. Isocucurbitacin B can be used for the research of cancer, such as glioma[1][2].

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Isocucurbitacin B

Isocucurbitacin B Chemical Structure

CAS No. : 17278-28-3

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Isocucurbitacin B Related Antibodies

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

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Akt Akt1 Akt2 Akt3

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p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

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STAT3 STAT5 STAT6 STAT1

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Description

Isocucurbitacin B is a natural terpenoid compound found in Pedicellus Melo. Isocucurbitacin B can inhibit the PI3K/AKT, MAPK, and STAT3 signaling pathways and downregulate CAV1 expression. Isocucurbitacin B can inhbit cancer cell proliferation, migration and invision. Isocucurbitacin B can induce apoptosis and cause G2/M phase arrest. Isocucurbitacin B can decrease intracellular cholesterol and PH levels and increase intracellular calcium levels. Isocucurbitacin B can be used for the research of cancer, such as glioma[1][2].
IC50 & Target[1]

STAT3

 

In Vitro

Isocucurbitacin B (0.001-25 μmol/L; 12-24 h) inhibits the proliferation of U251 and U87 cells[1][2].
Isocucurbitacin B (0-1 μmol/L; 24 h) induces U251 and GL261 cell apoptosis[1][2].
IsoCuB (0-1 μmol/L; 24 hours) blocks the cell cycle at the G2/M phase, reducing cyclin A2 and CDK1 expression in U251 cells[2].
Isocucurbitacin B (0-0.1 μmol/L; 12-36 hours) inhibits U251 and GL261 cells migration and invasion, reducing MMP2, N-cadherin, and vimentin levels[1][2].
Isocucurbitacin B (0-0.1 μmol/L; 24 h) inhibits U251 and GL261 cells invasion[1][2].
Isocucurbitacin B (0-1 μmol/L; 24 h) inhibits the activation of PI3K/AKT, MAPK, and STAT3 signaling pathways in U251 cells[1].
Isocucurbitacin B (0-1 μmol/L; 24 h) downregulates pdk1, RXRα, PPAα, and Bcl-2 mRNA expressions in U251 cells[1].
Isocucurbitacin B (0-1 μmol/L; 24 hours) decreases intracellular cholesterol and PH levels and increases intracellular calcium levels in U251 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Isocucurbitacin B Related Antibodies

Cell Proliferation Assay[12]

Cell Line: U251 and U87 cells
Concentration: 0.001, 0.01, 0.1, 0.5, 1, 5, 25 μmol/L
Incubation Time: 12 h, 24 h
Result: Inhibited U251 and U87 cell proliferation in a concentration- and time-dependent manner.

Apoptosis Analysis[12]

Cell Line: U251 and GL261 cells
Concentration: 0, 0.1, 0.5, 1 μmol/L
Incubation Time: 24 h
Result: Promoted the apoptosis of U251 and GL261 cells in a concentration-dependent manner. Increaseed BCL-2 amd BAX levels.

Cell Migration Assay [12]

Cell Line: U251 and GL261
Concentration: 0, 0.001, 0.01, 0.005, 0.1 μmol/L
Incubation Time: 12 h, 24 h, 36 h
Result: Inhibited the migration of U251 and GL 261 cells in a concentration- and time-dependent manner.

Cell Invasion Assay[12]

Cell Line: U251 and GL261
Concentration: 0, 0.001, 0.01, 0.05, 0.1 μmol/L
Incubation Time: 24 h
Result: Inhibited the invasion of U251 and GL261 cells in a concentration-dependent manner.

Western Blot Analysis[1]

Cell Line: U251
Concentration: 0, 0.1, 0.5, 1 μmol/L
Incubation Time: 24 h
Result: Inhibited the activation of the PI3K/AKT, MAPK, and STAT3 signaling pathways by decreasing the levels of p-MAPK1/3, total PI3K, t-AKT, PDK1, and p-STAT3 proteins.

Real Time qPCR[1]

Cell Line: U251
Concentration: 0, 0.1, 0.5, 1 μmol/L
Incubation Time: 24 h
Result: Downregulated the mRNA expressions of PDK1, RXRα, PPARα, and Bcl-2 in U251 cells.

Cell Cycle Analysis[2]

Cell Line: U251 cells
Concentration: 0, 0.1, 0.5, 1 μmol/L
Incubation Time: 24 hours
Result: Blocked the cell cycle of U251 cells at the G2/M phase in a concentration-dependent manner and reduced expression levels of cyclin A2 and CDK1.
In Vivo

Isocucurbitacin B (2 mg/kg;i.p.; daily for 18 days from day 6) inhibits tumor growth in U251 cell-transplanted BALB/c nude mice[1].
Isocucurbitacin B (0.025-0.05 mg/L; 72 hours) reduces tumor burden in glioma cell-induced zebrafish[2].
Isocucurbitacin B (1 mg/kg; intraperitoneal; daily; 21 days from day 7) inhibits glioma growth in GL261 cell-induced glioma in C57BL/6 mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six-week-old specific pathogen-free (SPF) BALB/c nude mice transplanted with U251 cells [1]
Dosage: 2 mg/kg
Administration: Intraperitoneally injection; daily; 18 days from day 6
Result: Tumor growth was significantly inhibited. No significant effect on body weight was observed.
Animal Model: C57BL/6 (Male, 5 weeks old)[2]
Dosage: 1 mg/kg
Administration: intraperitoneal; daily; 27 days
Result: Tumor volume was significantly reduced, and cell proliferation and glial fibrillary acidic protein expression were decreased.
Animal Model: Glioma cell-induced Zebrafish[2]
Dosage: 0.025 mg/L, 0.05 mg/L
Administration: In water; 72 hours
Result: Fluorescence intensity was significantly reduced, indicating a reduction in tumor burden.
Molecular Weight

558.70

Formula

C32H46O8
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@@]([C@]([C@@](C)(O)C(/C=C/C(C)(C)OC(C)=O)=O)([H])[C@H](O)C1)(CC([C@]3(C)[C@@]2([H])CC=C4[C@@]3([H])CC([C@@H](O)C4(C)C)=O)=O)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation
References
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Isocucurbitacin B Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isocucurbitacin B17278-28-3PI3KAktp38 MAPKSTATApoptosisPhosphoinositide 3-kinasePKBProtein kinase BGlioblastoma,PDX model,network pharmacology,drug sensitivity,Orthotopic glioma mouse model,Caveolin 1,hsa-mir-1286a,Anoikis,glioma,Isocucurbitacin BInhibitorinhibitorinhibit

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