Closthioamide
Closthioamide is a potent inhibitor of bacterial DNA gyrase and highly active against Ec, MRSA, VRE and Mv), with MICs of 9.00 μM, 0.58 μM, 0.58 μM and 72.03 μM respectively.
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- CAS 番号: 1227367-59-0
- 分子式: C29H38N6O2S6
- 分子量:695.04
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Bacterial DNA gyrase[1].
MIC: 9.00 μM (Ec), 0.58 μM (MRSA), 0.58 μM (VRE), 72.03 μM (Mv)[2].
Closthioamide is a potent inhibitor of bacterial DNA gyrase, isolated from the strictly anaerobic banterium Clostridium cellulolyticum and belongs to a new class of natural products[1]. In a standardized antimicrobial assay, it is found that closthioamide is highly active against a pathogenic, methicillin-resistant Staphylococcus aureus (MRSA) strain with a minimum inhibitory concentration (MIC) of 0.4 μg/mL-1 (0.58 μM). Closthioamide is even active against vancomycin-resistant Enterococcus faecalis (VRE) with the same low MIC value, and is thus significantly more potent against these bacteria than ciprofloxacin, the standard drugused against VRE, with remarkable strain selectivity. Furthermore, in a standardized cytotoxicity assay, closthioamide shows moderate antiproliferative and cytotoxic effects[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 1227367-59-0
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分子量 695.04
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分子式 C29H38N6O2S6
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SMILES
S=C(C1=CC=C(O)C=C1)NCCC(NCCC(NCCCNC(CCNC(CCNC(C2=CC=C(O)C=C2)=S)=S)=S)=S)=S
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Structure Classification
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Initial Source
the Strictly Anaerobic Bacterium Clostridium cellulolyticum
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
The MIC is determined for the laboratory strains as follows. The assays are performed in sterile 96-well polystyrene microplates in 200 mL of cation-adjusted Mueller-Hinton (MH) broth. A serial dilution of the compound to test is made in 100 mL of medium and inoculated with 100 mL of fresh cell culture containing ~105 cfu/mL and incubated at 37°C for 20 h. The MIC is defined as the lowest concentration of antibiotic that inhibit visible growth[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
純度とドキュメンテーション
参考文献
[1]. Chiriac AI, et al. Mode of action of closthioamide: the first member of the polythioamide class of bacterial DNA gyrase inhibitors. J AntimicrobChemother. 2015Sep;70(9):2576-88. [Content Brief]
[2]. Lincke T, et al. Closthioamide: an unprecedented polythioamide antibiotic from the strictly anaerobic bacterium Clostridium cellulolyticum.Angew Chem Int Ed Engl. 2010 Mar 8;49(11):2011-3. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Closthioamide 関連分類
HY-101472 関連分類
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