Colibactin 742

Colibactin 742 (100 nM-100 μM; 1.5 hours) induces stable DNA interstrand crosslinks (ICLs) using linearized pUC19 DNA as a substrate at both pH 5.0 and pH 7.0, and its activity is superior to Colibactin 770 (3a) at pH 5.0^[1].
Colibactin 742 (1-100 μM; 4 hours treatment followed by 20 hours incubation) increases the number of FANCD2 and γH2AX foci-positive cells by 4 times at 11 μM in HeLa cells, and induces cell death at >33 μM^[1].
Colibactin 742 (24 nM-100 μM; 72 hours) is cytotoxic to HeLa cells with an IC₅₀ of 5.2 μM^[1].
Colibactin 742 (10 μM, 100 μM; 4 hours, 12 hours) activates the p53 signaling pathway and induces cell cycle arrest, senescence, ER stress, and the IL-1 inflammatory pathway in FHC cells, upregulating the expression of genes such as CDKN1A, BTG2, and FAS^[2].
Colibactin 742 (20 μM; 48 hours/10 times, with 48-96 hour intervals) upregulates BRCA1 and Fanconi in HCT 116 cells." Anemia, mismatch repair (MMR), and IFNL1-related pathway gene expression increase T>N ??single-base substitutions (SBS) and short insertion-deletion (Indel) mutations^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.