SDUY127
SDUY127 is a bivalent SHP2 inhibitor with an IC50 value of 16 nM. SDUY127 binds simultaneously to the tunnel allosteric site and latch allosteric site of SHP2 at a 1:1 stoichiometric ratio, stabilizes the protein in an inactive conformation, and induces sustained inhibition of the MAPK signaling pathway. SDUY127 can be used in research related to leukemia and hepatocellular carcinoma.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C58H69Cl2N13O9S
- 分子量:1195.22
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
SDUY127 (1-3 μM; 48 h) inhibits MV4-11 leukemia cell proliferation by 33% at 1 μM and 79% at 3 μM after 48 h of treatment[1].
SDUY127 (10-30 μM; 48 h) inhibits Hep3B hepatocellular carcinoma cell proliferation by 23% at 10 μM and 84% at 30 μM after 48 h of treatment[1].
SDUY127 (3 μM; 15 min; 2 h) induces sustained suppression of ERK phosphorylation in MV4-11 cells, maintaining ~76% inhibition at 2 h post-treatment[1].
SDUY127 (3 μM; 15 min; 2 h) induces transient suppression of ERK phosphorylation in Hep3B cells, with inhibition decreasing from ~76% at 15 min to ~48% at 2 h post-treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MV4-11 leukemia cells
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Concentration:3 μM
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Incubation Time:15 min; 2 h
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Result:Suppressed pERK levels by ~79% at 15 min.
Maintained pERK suppression at ~76% at 2 h.
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Cell Line:Hep3B hepatocellular carcinoma cells
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Concentration:3 μM
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Incubation Time:15 min; 2 h
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Result:Suppressed pERK levels by ~76% at 15 min.
Decreased pERK inhibition to ~48% at 2 h.
化学情報
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分子量 1195.22
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分子式 C58H69Cl2N13O9S
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SMILES
COC1=C(C=CC=C1C(N2C3=C4C=CC=C3)=NN=C2N(C4=O)CC5=CC=CC=C5Cl)OCCOCCOCCOCCN6CCN(CC6)C(CCCNC(CC(NC7=CC=CC(SC8=C(N)N=C(N9CCC(C)(CC9)N)C=N8)=C7Cl)=O)=O)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Liver Cancer Modeling
Liver cancer can be classified into primary liver cancer and secondary liver cancer. Secondary liver cancer is the metastatic liver cancer. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and fibrolamellar HCC, of which HCC is the most common form, accounting for approximately 90% of primary liver cancers[1]. HCC mouse models include chemical agent-induced models, transplanted tumor models, and genetic engineered models.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
SDUY127 関連分類
HY-183936 関連分類
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