VLS-1272
Based on 1 Customer Validation
VLS-1272 (Compound 22) is an orally active KIF18A inhibitor that binds to the KIF18A-microtubule complex in an ATP-noncompetitive manner (IC50 = 41 nM), blocking its ATPase activity and inhibiting microtubule translocation. This leads to abnormal accumulation of KIF18A at spindle poles, disrupting chromosome alignment and inducing mitotic arrest and apoptosis in CINHigh tumor cells (e.g., ovarian cancer OVCAR-3, breast cancer JIMT-1). VLS-1272 is a promising candidate for anti-tumor research.
For research use only. We do not sell to patients.
- Purity: 99.63%
- CAS No.: 2914878-00-3
- Formula: C27H35N3O5S2
- Molecular Weight:545.71
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All Kinesin Isoforms
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Biological Activity
IC50: 9.4 nM (KIF18A microtubule-dependent ATPase activity)[1]
VLS-1272 (Compound 22; 7 days) inhibits JIMT-1, NIH-OVCAR3 and HCC-15 cells viability with IC50s of 0.0078, 0.0097 and 0.011 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCC-15, JIMT-1 and NIH-OVCAR3
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Concentration:
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Incubation Time:7 days
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Result:Inhibited cell viability with IC50s of 0.0078, 0.0097 and 0.011 μM against JIMT-1, NIH-OVCAR3 and HCC-15 cells, respectively.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:SCID Beige mice, HCC15 tumor model[1]
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Dosage:10, 30 and 60 mg/kg
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Administration:PO, twice a day for 1 month
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Result:Inhibited tumor growth by 30±15%, 72±6% and 82±9% at 10, 30 and 60 mg/kg, respectively.
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Animal Model:Balb/C nude mice, OVCAR3 tumor model[1]
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Dosage:10, 30 and 60 mg/kg
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Administration:PO, once a day for 1 month
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Result:Inhibited tumor growth by 24±26%, 72±17% and 82±10% at 10, 30 and 60 mg/kg, respectively.
Chemical Information
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CAS No. 2914878-00-3
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Appearance Solid
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Molecular Weight 545.71
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Formula C27H35N3O5S2
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Color White to off-white
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SMILES
O=S(C1=CC(C(N2CC3(CCC4(CC4)CC3)C5=CC(NS(C)(=O)=O)=CC=C52)=O)=CC=C1)(NC(C)(C)C)=O
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Synonyms
KIF18A-IN-7
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 25 mg/mL (45.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8325 mL | 9.1624 mL | 18.3248 mL | 45.8119 mL |
| 5 mM | 0.3665 mL | 1.8325 mL | 3.6650 mL | 9.1624 mL | |
| 10 mM | 0.1832 mL | 0.9162 mL | 1.8325 mL | 4.5812 mL | |
| 15 mM | 0.1222 mL | 0.6108 mL | 1.2217 mL | 3.0541 mL | |
| 20 mM | 0.0916 mL | 0.4581 mL | 0.9162 mL | 2.2906 mL | |
| 25 mM | 0.0733 mL | 0.3665 mL | 0.7330 mL | 1.8325 mL | |
| 30 mM | 0.0611 mL | 0.3054 mL | 0.6108 mL | 1.5271 mL | |
| 40 mM | 0.0458 mL | 0.2291 mL | 0.4581 mL | 1.1453 mL |
VLS-1272 Related Classifications
HY-153066 Related Classifications
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