Cat. No.: HY-13251

Silvestrol

(Synonyms: (-)-Silvestrol)

53 Cited Publications

Customer Review

Based on 53 publication(s) in Google Scholar

Silvestrol is a eukaryotic translation initiation factor 4A (eIF4A) inhibitor isolated from Agave americana Linn.. Silvestrol induces autophagy and caspase-mediated apoptosis.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 697235-38-4
Formula: C₃₄H₃₈O₁₃
Molecular Weight:654.66

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Silvestrol Chemical Structure

CAS No.: 697235-38-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (53)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Silvestrol is a eukaryotic translation initiation factor 4A (eIF4A) inhibitor isolated from Agave americana Linn.. Silvestrol induces autophagy and caspase-mediated apoptosis^[1]^[2]^[3].

IC₅₀ & Target^[3]

eIF4

Cellular Effect

Cell Line	Type	Value	Description	References
Cancer cell lines	ED₅₀	1.2 nM Compound: 1	Cytotoxicity against human breast cancer cells Cytotoxicity against human breast cancer cells	[PMID: 22817615]
Cancer cell lines	ED₅₀	1.2 nM Compound: 1	Cytotoxicity against human lung cancer cells Cytotoxicity against human lung cancer cells	[PMID: 22817615]
HeLa	IC₅₀	>20 μM Compound: 7	Inhibition of NF-kappaB p65 isolated from nuclear extract of human HeLa cells assessed as blockade of binding to biotinylated consesus sequence by chemiluminescence assay Inhibition of NF-kappaB p65 isolated from nuclear extract of human HeLa cells assessed as blockade of binding to biotinylated consesus sequence by chemiluminescence assay	[PMID: 20939540]
HeLa	IC₅₀	0.02 μM Compound: 8	Antiproliferative activity against human HeLa cells assessed as cell growth inhibition by [35S] methionine incorporation assay Antiproliferative activity against human HeLa cells assessed as cell growth inhibition by [35S] methionine incorporation assay	[PMID: 33592144]
HepG2	CC₅₀	13.4 nM Compound: 2a	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 38127656]
HepG2	CC₅₀	180.3 nM Compound: 2a	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 38127656]
HL-60	IC₅₀	4 nM Compound: 8	Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay	[PMID: 33592144]
HT-29	ED₅₀	0.0007 μM Compound: 7	Cytotoxicity against human HT-29 cells after 3 days by sulforhodamine B assay Cytotoxicity against human HT-29 cells after 3 days by sulforhodamine B assay	[PMID: 20939540]
HT-29	ED₅₀	0.7 nM Compound: 7	Cytotoxicity against human HT-29 cells after 3 days by sulforhodamine B assay Cytotoxicity against human HT-29 cells after 3 days by sulforhodamine B assay	[PMID: 20939540]
HT-29	IC₅₀	0.004 μM Compound: Silvestrol	Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay	[PMID: 21513293]
Kasumi 1	IC₅₀	24 nM Compound: Silvestrol	Cytotoxicity against human Kasumi-1 cells incubated for 48 hrs by MTS assay Cytotoxicity against human Kasumi-1 cells incubated for 48 hrs by MTS assay	[PMID: 27983842]
LNCaP	ED₅₀	1.5 nM Compound: 13	Growth inhibition of human LNCAP cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test Growth inhibition of human LNCAP cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test	[PMID: 19161316]
Lu1	ED₅₀	1.2 nM Compound: 13	Growth inhibition of human Lu1 cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test Growth inhibition of human Lu1 cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test	[PMID: 19161316]
MCF7	ED₅₀	1.5 nM Compound: 13	Growth inhibition of human MCF7 cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test Growth inhibition of human MCF7 cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test	[PMID: 19161316]
MDA-MB-231	EC₅₀	0.8 nM Compound: 1	Inhibition of eIF4FA in human MDA-MB-231 cells transfected with c-myc- 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay Inhibition of eIF4FA in human MDA-MB-231 cells transfected with c-myc- 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay	[PMID: 23025805]
MDA-MB-231	EC₅₀	3 nM Compound: 1	Growth inhibition of human MDA-MB-231 cells incubated for 72 hrs by MTS assay Growth inhibition of human MDA-MB-231 cells incubated for 72 hrs by MTS assay	[PMID: 23025805]
MDA-MB-231	EC₅₀	7 nM Compound: 1	Inhibition of eIF4FA in human MDA-MB-231 cells transfected with tubulin 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay Inhibition of eIF4FA in human MDA-MB-231 cells transfected with tubulin 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay	[PMID: 23025805]
MDA-MB-435	IC₅₀	0.006 μM Compound: Silvestrol	Cytotoxicity against human MDA-MB-435 cells after 72 hrs by MTS assay Cytotoxicity against human MDA-MB-435 cells after 72 hrs by MTS assay	[PMID: 21513293]
MV4-11	IC₅₀	11 nM Compound: Silvestrol	Cytotoxicity against human MV4-11 cells incubated for 48 hrs by MTS assay Cytotoxicity against human MV4-11 cells incubated for 48 hrs by MTS assay	[PMID: 27983842]
NB-4	IC₅₀	4 nM Compound: 8	Antiproliferative activity against human NB-4 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay Antiproliferative activity against human NB-4 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay	[PMID: 33592144]
OCI-AML2	IC₅₀	4 nM Compound: 8	Antiproliferative activity against human OCI-AML2 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay Antiproliferative activity against human OCI-AML2 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay	[PMID: 33592144]
OCI-AML-3	IC₅₀	4 nM Compound: 8	Antiproliferative activity against human OCI-AML-3 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay Antiproliferative activity against human OCI-AML-3 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay	[PMID: 33592144]
U-937	IC₅₀	4 nM Compound: 8	Antiproliferative activity against human U-937 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay Antiproliferative activity against human U-937 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay	[PMID: 33592144]

In Vitro

Silvestrol is a specific eIF4A-targeting translation inhibitor. Silvestrol exhibits significant cytotoxic activity against many human cancer cell lines, such as lung, prostate, and breast cancer with IC₅₀ values ranging from 1 to 7 nM^[1].
Silvestrol significantly reduces the number of LNCaP cell colonies. Silvestrol (30 nM, 120 nM) induces apoptosis in LNCaP cells, through the mitochondrial pathway. Apaf-1, Caspase-2, caspase-9, and caspase-10 are involved in Silvestrol-induced apoptosis but caspase-3 and 7 are not^[2].
Silvestrol induces caspase-3 activation and apoptotic cell death in a time- and dose-dependent manner. Silvestrol-mediated cell death is attenuated in ATG7-null mouse embryonic fibroblasts (MEFs) lacking a functional autophagy protein^[3].
Silvestrol (50 nM) exerts an immediate inhibitory effect and causes near-static cell index compared with the control cells. Silvestrol (6.25 nM) enhances proliferation more than the vehicle control-treated cells, whereas a higher concentration of Silvestrol (50 nM) can inhibit cell proliferation. Silvestrol and episilvestrol display synergistic effects in combination with CDDP^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Silvestrol (1.5 mg/kg, i.p.) does not adversely affect production of human IgG by xenografted B-lymphocytes in mice. Silvestrol significantly prolongs survival compared to vehicle. There is no such lymphocyte infiltration detected in the spleens of any of the Silvestrol-treated mice, and nor do these animals exhibit any other obvious signs of lymphoma upon necropsy^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 697235-38-4
Appearance Solid
Molecular Weight 654.66
Formula C₃₄H₃₈O₁₃
Color White to off-white
SMILES

O=C([C@H]([C@H]1C2=CC=CC=C2)[C@@H](O)[C@]3(O)[C@@]1(C4=CC=C(OC)C=C4)OC5=CC(O[C@@H]6O[C@@H]([C@H](O)CO)CO[C@H]6OC)=CC(OC)=C35)OC
Synonyms

(-)-Silvestrol
Structure Classification
- Others
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (53)

All WBIF

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Journal Impact Factor

Nature

RNA G-quadruplexes cause eIF4A-dependent oncogene translation in cancer. [Abstract]

2014 Sep 4;513(7516):65-70. PMID: 25079319

Silvestrol purchased from MedChemExpress. Usage Cited in: Nature. 2014 Sep 4;513(7516):65-70. [Abstract]

Immunoblots of lysates from human T-ALL lines treated with Silvestrol (25 nM, 24h) and probed as indicated.
Cancer Discov

Targeting Translation Initiation Bypasses Signaling Crosstalk Mechanisms That Maintain High MYC Levels in Colorectal Cancer. [Abstract]

2015 Jul;5(7):768-781. PMID: 25934076

Silvestrol purchased from MedChemExpress. Usage Cited in: Cancer Discov. 2015 Jul;5(7):768-781. [Abstract]

Immunoblots of four colorectal cell lines upon treatment with increasing concentration of Silvestrol or solvent control (n=2).
Blood

Inhibiting CARD11 translation during BCR activation by targeting the eIF4A RNA helicase. [Abstract]

2014 Dec 11;124(25):3758-67. PMID: 25320244

Silvestrol purchased from MedChemExpress. Usage Cited in: Blood. 2014 Dec 11;124(25):3758-67. [Abstract]

(A)Protein lysates from human splenic B cells are harvested 24 hours after treatment with Isotype + Vehicle, Isotype + 10 nM Silvestrol, anti-BCR + Vehicle, or anti-BCR + 10 nM Silvestrol and incubated with m7GTP Sepharose beads overnight at 4°C. Western blot analysis is used to observe eIF4A and eIF4E binding to m7GTP. Data are representative of 3e independent experiments. (B) Twenty-four hours after treatment, cells are incubated with 1 mCi L-[³⁵S]methionine and L-[³⁵S]cy
Autophagy

Targetome analysis of chaperone-mediated autophagy in cancer cells. [Abstract]

2019 Sep;15(9):1558-1571. PMID: 30821613
Nat Commun

Modeling NK-cell lymphoma in mice reveals its cell-of-origin and microenvironmental changes and identifies therapeutic targets. [Abstract]

2024 Oct 22;15(1):9106. PMID: 39438472
Nat Commun

An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis. [Abstract]

2019 Jul 1;10(1):2901. PMID: 31263101
Mol Cell

Localization of a TORC1-eIF4F translation complex during CD8+ T cell activation drives divergent cell fate. [Abstract]

2022 Jul 7;82(13):2401-2414.e9. PMID: 35597236
Mol Cell

Regulation of PTEN translation by PI3K signaling maintains pathway homeostasis. [Abstract]

2021 Feb 18;81(4):708-723.e5. PMID: 33606974
Nucleic Acids Res

Eukaryotic translation initiation factor 4AII contributes to microRNA-122 regulation of hepatitis C virus replication. [Abstract]

2018 Jul 6;46(12):6330-6343. PMID: 29669014
Nucleic Acids Res

eIF4A alleviates the translational repression mediated by classical secondary structures more than by G-quadruplexes. [Abstract]

2018 Apr 6;46(6):3075-3087. PMID: 29471358
Cell Rep Med

Reprograming immunosuppressive microenvironment by eIF4G1 targeting to eradicate pancreatic ductal adenocarcinoma. [Abstract]

2024 Oct 15;5(10):101731. PMID: 39303711
Clin Cancer Res

Target-Based Screening against eIF4A1 Reveals the Marine Natural Product Elatol as a Novel Inhibitor of Translation Initiation with In Vivo Antitumor Activity. [Abstract]

2018 Sep 1;24(17):4256-4270. PMID: 29844128

Silvestrol purchased from MedChemExpress. Usage Cited in: Clin Cancer Res. 2018 Sep 1;24(17):4256-4270. [Abstract]

Western blot showing protein expression of translationally regulated genes in DLBCL cells treated with DMSO (D), 50 nM Silvestrol (S) or 5 μM elatol for 16h. Representative images, n=3.
Dev Cell

Autophagy-dependent splicing control directs translation toward inflammation during senescence. [Abstract]

2025 Feb 3;60(3):364-378.e7. PMID: 39510077
Oncogene

RBM12 drives PD-L1-mediated immune evasion in hepatocellular carcinoma by increasing JAK1 mRNA translation. [Abstract]

2024 Oct;43(41):3062-3077. PMID: 39187545
Oncogene

Tumor suppressor Pdcd4 attenuates Sin1 translation to inhibit invasion in colon carcinoma. [Abstract]

2017 Nov 9;36(45):6225-6234. PMID: 28692058

Silvestrol purchased from MedChemExpress. Usage Cited in: Oncogene. 2017 Nov 9;36(45):6225-6234. [Abstract]

Sin1 protein level decreases in cells treated with silvestrol. HT29-P or HCT116 cells were treated with indicated concentration of Silvestrol for 24 h. The ratio of Sin1/GAPDH and Snail/GAPDH in DMSO (0 nM Silvestrol) treated cells is designated as 1.0.
EMBO J

miRISC and the CCR4-NOT complex silence mRNA targets independently of 43S ribosomal scanning. [Abstract]

2016 Jun 1;35(11):1186-203. PMID: 27009120

Silvestrol purchased from MedChemExpress. Usage Cited in: EMBO J. 2016 Jun 1;35(11):1186-203. [Abstract]

The inhibitory effect of Silvestrol in translation is observed despite a two to five fold increase of reporter mRNA levels in HEK293 cells
Mol Med

Novel eIF4A1 inhibitors with anti-tumor activity in lymphoma. [Abstract]

2022 Sep 4;28(1):101. PMID: 36058921
Cell Rep

Enhanced bypass of PD-L1 translation reduces the therapeutic response to mTOR kinase inhibitors. [Abstract]

2023 Jul 4;42(7):112764. PMID: 37405918
Cell Rep

Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome. [Abstract]

2021 Oct 12;37(2):109806. PMID: 34644561
Cell Rep

Negative charge in the RACK1 loop broadens the translational capacity of the human ribosome. [Abstract]

2021 Sep 7;36(10):109663. PMID: 34496247
Cell Rep

Transcriptional programming drives Ibrutinib-resistance evolution in mantle cell lymphoma. [Abstract]

2021 Mar 16;34(11):108870. PMID: 33730585
Cell Mol Life Sci

Targeted inhibition of eIF4A suppresses B-cell receptor-induced translation and expression of MYC and MCL1 in chronic lymphocytic leukemia cells. [Abstract]

2021 Sep;78(17-18):6337-6349. PMID: 34398253
Mol Cancer Res

Deubiquitinase OTUD6B Isoforms Are Important Regulators of Growth and Proliferation. [Abstract]

2017 Feb;15(2):117-127. PMID: 27864334
BMC Biol

Establishment of an imaging-based screening pipeline for the identification of human ribosome biogenesis inhibitors. [Abstract]

2025 Oct 21;23(1):315. PMID: 41121194
Cancers (Basel)

Identification of Cardiac Glycosides as Novel Inhibitors of eIF4A1-Mediated Translation in Triple-Negative Breast Cancer Cells. [Abstract]

2020 Aug 4;12(8):2169. PMID: 32759815
Antiviral Res

Identification of structurally re-engineered rocaglates as inhibitors against hepatitis E virus replication. [Abstract]

2022 Aug:204:105359. PMID: 35728703
Antiviral Res

The natural compound silvestrol inhibits hepatitis E virus (HEV) replication in vitro and in vivo. [Abstract]

2018 Sep:157:151-158. PMID: 30036559

Silvestrol purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2018 Sep:157:151-158. [Abstract]

Immunofluorescence staining of HEVp6 transfected Huh7.5 cells treated with Silvestrol (low concentration: 2.5 nM and medium concentration: 10 nM), ICN-1229 or DMSO 4 h after transfection for 42 h.
Antiviral Res

Broad-spectrum antiviral activity of the eIF4A inhibitor silvestrol against corona- and picornaviruses. [Abstract]

2018 Feb:150:123-129. PMID: 29258862

Silvestrol purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2018 Feb:150:123-129. [Abstract]

Huh-7 cells are infected with HCoV-229E at an MOI of 3 and treated with the indicated concentrations of silvestrol. Cell lysates are prepared at 12 h p.i. and 48 h p.i. as indicated and the accumulation of viral N and nsp8 proteins is determined by Western blot analysis.

Silvestrol purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2018 Feb:150:123-129. [Abstract]

MRC-5 cells are infected with HCoV-229E at an MOI of 3 and treated with the indicated concentrations of silvestrol. Cell lysates are prepared at 12 h p.i. and 48 h p.i. as indicated and the accumulation of viral N and nsp8 proteins is determined by Western blot analysis.
Antiviral Res

The natural compound silvestrol is a potent inhibitor of Ebola virus replication. [Abstract]

2017 Jan:137:76-81. PMID: 27864075

Silvestrol purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2017 Jan:137:76-81. [Abstract]

PIM1 levels are strongly decreased (to almost below detection limits) in the presence of 10 nM Silvestrol. This inhibitory effect persists for at least 5 days using a single dose of Silvestrol, without affecting cellular β-Actin levels.
Front Biosci (Landmark Ed)

Activation of the MEK/ERK Pathway Mediates the Inhibitory Effects of Silvestrol on Nasopharyngeal Carcinoma Cells via RAP1A, HK2, and GADD45A. [Abstract]

2024 Apr 23;29(4):160. PMID: 38682208
J Cell Physiol

Amyloid precursor protein (APP) affects global protein synthesis in dividing human cells. [Abstract]

2015 May;230(5):1064-74. PMID: 25283437
J Cell Sci

Activation of the mitochondrial unfolded protein response regulates the dynamic formation of stress granules. [Abstract]

2025 May 1;138(9):jcs263548. PMID: 39463355
Viruses

Silvestrol Inhibits Chikungunya Virus Replication. [Abstract]

2018 Oct 30;10(11):592. PMID: 30380742

Silvestrol purchased from MedChemExpress. Usage Cited in: Viruses. 2018 Oct 30;10(11):592. [Abstract]

Infection of 293T cells with CHIKV-mCherry in the presence of 50 nM silvestrol. Western blot analysis of cell lysates prepared from 293T cells infected with CHIKV-mCherry.
Viruses

Inhibition of Hepatitis E Virus Spread by the Natural Compound Silvestrol. [Abstract]

2018 Jun 2;10(6):301. PMID: 29865243

Silvestrol purchased from MedChemExpress. Usage Cited in: Viruses. 2018 Jun 2;10(6):301. [Abstract]

Representative Western Blot of cellular lysates derived from cells treated with either 2 nM or 50 nM Silvestrol. DMSO treatment serves as control.
Viruses

Inhibition of Zika Virus Replication by Silvestrol. [Abstract]

2018 Mar 27;10(4):149. PMID: 29584632

Silvestrol purchased from MedChemExpress. Usage Cited in: Viruses. 2018 Mar 27;10(4):149. [Abstract]

A549 cells are infected with ZIKV U and treated with the indicated amount of Silvestrol. Cell lysates of the indicated time points are analyzed by Western blot with specific antibodies against NS1 and β-actin.

Silvestrol purchased from MedChemExpress. Usage Cited in: Viruses. 2018 Mar 27;10(4):149. [Abstract]

A549 cells are infected with ZIKV FP and treated with the indicated amount of Silvestrol. Cell lysates of the indicated time points are analyzed by Western blot with specific antibodies against NS1 and β-actin.
Viruses

Stress Granule-Inducing Eukaryotic Translation Initiation Factor 4A Inhibitors Block Influenza A Virus Replication. [Abstract]

2017 Dec 18;9(12):388. PMID: 29258238

Silvestrol purchased from MedChemExpress. Usage Cited in: Viruses. 2017 Dec 18;9(12):388. [Abstract]

Western blotting analysis of A549 cell lysates obtained at the indicated times post-infection with the PR8 strain of IAV and treated with 320 nM silvestrol (Sil.) or 20 nM pateamine A (PatA) at 4 hpi or the equivalent time after mock infection.
Cancer Med

Translational Inhibition of Slug by Pdcd4 Contributes to Invasion Inhibition in Colorectal Cancer Cells. [Abstract]

2025 Aug;14(15):e71145. PMID: 40785580
Clin Transl Radiat Oncol

The eIF4A inhibitor silvestrol sensitizes T-47D ductal breast carcinoma cells to external-beam radiotherapy. [Abstract]

2020 Jul 24:24:123-126. PMID: 32875125
Cancer Manag Res

Effective Inhibition of MYC-Amplified Group 3 Medulloblastoma Through Targeting EIF4A1. [Abstract]

2020 Dec 3:12:12473-12485. PMID: 33299354
J Invertebr Pathol

Identification of the in vitro antiviral effect of BmNedd2-like caspase in response to Bombyx mori nucleopolyhedrovirus infection. [Abstract]

2021 Jul:183:107625. PMID: 34058216
STAR Protoc

MATRIX platform to analyze translation machinery remodeling in glioblastoma cells. [Abstract]

2022 Dec 16;3(4):101919. PMID: 36595908
bioRxiv

2026 May 13.
bioRxiv

2025 Nov 13.
bioRxiv

Oxidative stress sensing by the translation elongation machinery promotes production of detoxifying selenoproteins. [Abstract]

2025 Oct 13:2025.10.13.682107. PMID: 41279241
bioRxiv

2023 Oct 26.
Dalhousie University

2022 Aug.
J Oncol

The eIF4A Inhibitor Silvestrol Blocks the Growth of Human Glioblastoma Cells by Inhibiting AKT/mTOR and ERK1/2 Signaling Pathway. [Abstract]

2022 May 30:2022:4396316. PMID: 35677890
Dalhousie University

2021 Oct.
Universität Würzburg

2020 Nov.
Elife

USP16 counteracts mono-ubiquitination of RPS27a and promotes maturation of the 40S ribosomal subunit. [Abstract]

2020 Mar 4:9:e54435. PMID: 32129764
University of Miami

2018 Jul.

Silvestrol purchased from MedChemExpress. Usage Cited in: University of Miami. 2018 Jul.

Western blot analysis of translationally regulates protein expression after 16h treatment.
Loyola University Chicago

2015 Aug.
University of Maryland

2014.

Silvestrol purchased from MedChemExpress. Usage Cited in: University of Maryland. 2014.

IgG positive B-cells display more significant modulation of CARD11 protein levels upon BCR activation and treatment with Silvestrol.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (152.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5275 mL

7.6376 mL

15.2751 mL

5 mM

0.3055 mL

1.5275 mL

3.0550 mL

10 mM

0.1528 mL

0.7638 mL

1.5275 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (3.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[2]

The cells are seeded at a density of 7×10⁴ cells/mL in 100-mm culture dishes and are treated with 30 nM or 120 nM concentrations of Silvestrol for 24 h^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[5]

Mice^[5]
Peripheral blood mononuclear cells (PBMC) are injected intraperitoneally (IP) into SCID mice depleted of murine natural killer (NK) cells by pretreatment (plus weekly re-treatment) with anti-asialo (GM1). Engraftment is confirmed by hu-IgG ELISA. Treatments with vehicle (30% hydroxypropyl-β-cyclodextrin) or Silvestrol (1.5 mg/kg every 48 hr IP) begin 2 weeks post-engraftment^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chambers JM, et al. Synthesis of biotinylated episilvestrol: highly selective targeting of the translation factors eIF4AI/II. Org Lett. 2013 Mar 15;15(6):1406-9. [Content Brief]

[2]. Kim S, et al. Silvestrol, a potential anticancer rocaglate derivative from Aglaia foveolata, induces apoptosis in LNCaP cells through the mitochondrial/apoptosome pathway without activation of executioner caspase-3 or -7. Anticancer Res. 2007 Jul-Aug;27(4B):2175-83. [Content Brief]

[3]. Chen WL, et al. Silvestrol induces early autophagy and apoptosis in human melanoma cells. BMC Cancer. 2016 Jan 13;16:17. [Content Brief]

[4]. Daker M, et al. Inhibition of nasopharyngeal carcinoma cell proliferation and synergism of CDDP with silvestrol and episilvestrol isolated from Aglaia stellatopilosa. Exp Ther Med. 2016 Jun;11(6):2117-2126. [Content Brief]

[5]. Patton JT, et al. The translation inhibitor silvestrol exhibits direct anti-tumor activity while preserving innate and adaptive immunity against EBV-driven lymphoproliferative disease. Oncotarget. 2015 Feb 20;6(5):2693-708. [Content Brief]

[6]. Wolfe AL, et al. RNA G-quadruplexes cause eIF4A-dependent oncogene translation in cancer. Nature. 2014 Sep 4;513(7516):65-70. [Content Brief]

[7]. Wiegering A, et al. Targeting Translation Initiation Bypasses Signaling Crosstalk Mechanisms That Maintain High MYC Levels in Colorectal Cancer. Cancer Discov. 2015 Jul;5(7):768-781. [Content Brief]

[8]. Todt D, et al. The natural compound silvestrol inhibits hepatitis E virus (HEV) replication in vitro and in vivo. Antiviral Res. 2018 Sep;157:151-158. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.5275 mL	7.6376 mL	15.2751 mL	38.1878 mL
	5 mM	0.3055 mL	1.5275 mL	3.0550 mL	7.6376 mL
	10 mM	0.1528 mL	0.7638 mL	1.5275 mL	3.8188 mL
	15 mM	0.1018 mL	0.5092 mL	1.0183 mL	2.5459 mL
	20 mM	0.0764 mL	0.3819 mL	0.7638 mL	1.9094 mL
	25 mM	0.0611 mL	0.3055 mL	0.6110 mL	1.5275 mL
	30 mM	0.0509 mL	0.2546 mL	0.5092 mL	1.2729 mL
	40 mM	0.0382 mL	0.1909 mL	0.3819 mL	0.9547 mL
	50 mM	0.0306 mL	0.1528 mL	0.3055 mL	0.7638 mL
	60 mM	0.0255 mL	0.1273 mL	0.2546 mL	0.6365 mL
	80 mM	0.0191 mL	0.0955 mL	0.1909 mL	0.4773 mL
	100 mM	0.0153 mL	0.0764 mL	0.1528 mL	0.3819 mL

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Silvestrol

MCE Bioactive Compound Library

Natural Product Library Plus

Publications Citing Use of MedChemExpress (MCE) Silvestrol

Biological Activity

Chemical Information

Publications (53)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Silvestrol

MCE Bioactive Compound Library

Natural Product Library Plus

Publications Citing Use of MedChemExpress (MCE) Silvestrol

Biological Activity

Chemical Information

Publications (53)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Silvestrol Related Classifications

HY-13251 Related Classifications