BC‐AZIDE
BC‐AZIDE (CLIP-AZIDE) is a CLIP-tag substrate that forms a covalent irreversible linkage with CLIP-tag proteins. When paired with conjugated fluorophores, BC‐AZIDE enables specific labeling of CLIP-tagged proteins in live and fixed cells. BC‐AZIDE can be used for research related to bioorthogonal click chemistry and intracellular orthogonal labeling.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- CAS No.: 2432950-51-9
- 화학식: C18H23N7O4
- 분자량:401.42
-
보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocol
- 순도&문서
- References
-
Help & FAQs
Biological Activity
제품 설명
In Vitro
BC-AZIDE (30 min) mediates SPAAC labeling of the nuclear envelope and actin fibers by DBCO-PEG4-5/6-TAMRA in live HeLa cells, enables SPAAC/IEDDA dual labeling of SNAP-/CLIP-tag fusion proteins in live cells with no obvious cross-reactivity, and also accomplishes two-color SPAAC/IEDDA labeling of CLIP-tag targets in fixed-permeabilized HeLa cells[2][3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 2432950-51-9
-
분자량 401.42
-
화학식 C18H23N7O4
-
SMILES
[N-]=[N+]=NCCOCCOCC(NCC1=CC=C(C=C1)COC2=NC(N)=CC=N2)=O
-
Synonyms
CLIP-AZIDE
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
References
[1]. Gayathri V, et al. Functionalization of Bacterial Cellulose and Related Surfaces Using a Facile Coupling Reaction by Thermoresponsive Catalyst. ACS biomaterials science & engineering. 2023 Feb 13;9(2):625-641. [Content Brief]
[2]. Krishnamurthy M, et al. Improved Hydrophobicity of a Bacterial Cellulose Surface: Click Chemistry in Action. ACS biomaterials science & engineering. 2020 Feb 10;6(2):879-888. [Content Brief]
[3]. Macias-Contreras M, et al. SNAP/CLIP-Tags and Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)/Inverse Electron Demand Diels-Alder (IEDDA) for Intracellular Orthogonal/Bioorthogonal Labeling. Bioconjugate chemistry. 2020 May 20;31(5):1370-1381. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BC‐AZIDE Related Classifications
HY-D2955 Related Classifications
Powered by Bioz