L-158809

The glioma/brain tumor 3D invasion assay measures outward migration and matrix invasion from multicellular tumor spheroids into a 3D extracellular matrix or organotypic brain slice. The readout is generated by time-lapse brightfield, fluorescence, confocal, or high-content imaging and quantified as invasion distance, invasion area, migration index, single-cell velocity, directionality, cumulative sprout length, or Z-direction invasion into brain tissue. Classic in vitro versions embed glioma or GBM spheroids in collagen I, Matrigel, collagen I/Matrigel, or collagen I/Matrigel/hyaluronic acid matrices, while ex vivo versions implant fluorescent GBM spheroids onto organotypic brain slices to model invasion in a preserved brain microenvironment.

Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by a progressive decline in cognitive functions and loss of specific types of neurons and synapses. Alzheimer's symptoms can be simulated in mice by injecting drugs (such as Aβ) or genetically modified.