LEI-401
Based on 3 publication(s) in Google Scholar
LEI-401 is a first-in-class, selective, and CNS-active NAPE-PLD (N-acylphosphatidylethanolamine phospholipase D) inhibitor, with an IC50 of 27 nM. LEI-401 modulates emotional behavior in mice.
For research use only. We do not sell to patients.
- Purity: 99.73%
- CAS No.: 2393840-15-6
- Formula: C24H31N5O2
- Molecular Weight:421.54
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Metabolism/Protease Compound Library
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Publications Citing Use of MedChemExpress (MCE) LEI-401
MoreAll Phospholipase Isoforms
More
Biological Activity
LEI-401 reduced a broad range of NAEs including anandamide in neuronal cells in a NAPE-PLD-dependent manner. LEI-401 (0.04-20 μM; 30?minutes) dose-dependently reduces the labeling of NAPE-PLD with an IC50 of 0.86 μM in hNAPE-PLD-transfected HEK293T cells. LEI-401 reduces NAE levels in Neuro-2a cells, but not in NAPE-PLD KO cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
? LEI-401 also activates HPA axis signaling[1].
? LEI-401 (10 mg/kg; p.o.) treatment shows the t1/2, Cmax, tmax, AUClast, and F values of 2.5 hours, 1370 ng/mL, 2 hours, 6760 h*ng/mL, and 25%, respectively[1].
? LEI-401 (30 mg/kg; i.p.) treatment show the Cmax, tmax, AUClast, and F values of 10300 ng/mL, 1 hour, 38600 h*ng/mL, and 48%, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male 7–12-week-old C57BL/6J mice[1]
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Dosage:30 mg/kg
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Administration:I.p.
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Result:Produced a significant increase in freezing as compared to vehicle.
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Animal Model:C57BL/6J mice[1]
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Dosage:10 mg/kg
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Administration:P.o. (Pharmacokinetic Analysis)
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Result:The t1/2, Cmax, tmax, AUClast, and F values were 2.5 hours, 1370 ng/mL, 2 hours, 6760 h*ng/mL, and 25%, respectively.
Chemical Information
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CAS No. 2393840-15-6
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Appearance Solid
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Molecular Weight 421.54
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Formula C24H31N5O2
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Color White to off-white
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SMILES
O=C(C1=NC(N2C[C@H](C3=CC=CC=C3)CCC2)=NC(N4C[C@@H](O)CC4)=C1)NCC5CC5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Nat Commun
Fmp30p is a mitochondrial phosphatidylinositol hydrolase that modulates CoQ biosynthesis. [Abstract]2026 May 30. PMID: 42218121 -
Neuron
In vivo endocannabinoid dynamics at the timescale of physiological and pathological neural activity. [Abstract]2021 Aug 4;109(15):2398-2403.e4. PMID: 34352214 -
Solvent & Solubility
DMSO : 100 mg/mL (237.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3723 mL | 11.8613 mL | 23.7225 mL | 59.3064 mL |
| 5 mM | 0.4745 mL | 2.3723 mL | 4.7445 mL | 11.8613 mL | |
| 10 mM | 0.2372 mL | 1.1861 mL | 2.3723 mL | 5.9306 mL | |
| 15 mM | 0.1582 mL | 0.7908 mL | 1.5815 mL | 3.9538 mL | |
| 20 mM | 0.1186 mL | 0.5931 mL | 1.1861 mL | 2.9653 mL | |
| 25 mM | 0.0949 mL | 0.4745 mL | 0.9489 mL | 2.3723 mL | |
| 30 mM | 0.0791 mL | 0.3954 mL | 0.7908 mL | 1.9769 mL | |
| 40 mM | 0.0593 mL | 0.2965 mL | 0.5931 mL | 1.4827 mL | |
| 50 mM | 0.0474 mL | 0.2372 mL | 0.4745 mL | 1.1861 mL | |
| 60 mM | 0.0395 mL | 0.1977 mL | 0.3954 mL | 0.9884 mL | |
| 80 mM | 0.0297 mL | 0.1483 mL | 0.2965 mL | 0.7413 mL | |
| 100 mM | 0.0237 mL | 0.1186 mL | 0.2372 mL | 0.5931 mL |
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