1. Metabolic Enzyme/Protease
  2. Phosphatase
  3. Lumigen APS-5

Lumigen APS-5 is a substrate of alkaline phosphatase (ALP). Lumigen APS-5 can be used to assess the activity of alkaline phosphatase (ALP).

For research use only. We do not sell to patients.

Lumigen APS-5 Chemical Structure

Lumigen APS-5 Chemical Structure

CAS No. : 193884-53-6

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Description

Lumigen APS-5 is a substrate of alkaline phosphatase (ALP). Lumigen APS-5 can be used to assess the activity of alkaline phosphatase (ALP)[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of Alkaline Phosphatase Activity[1]:
1. Mix testing samples (10 mg) with Lumigen APS-5 (100 μL) and vortexed for 10 s at the highest setting. For the control, 10 μL of distilled water.
2. The mixed samples are heat treated at 75℃ for 1 min in a water bath to inactivate nonbacterial ALP. After heat treatment, samples are placed in 96 well plates and measured immediately. The activity of ALP is measured by using a luminometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

491.84

Formula

C21H17ClNNa2O4PS

CAS No.
SMILES

CN(C1C/2C=CC=C1)C3=CC=CC=C3C2=C(OP(O[Na])(O[Na])=O)/SC4=CC=C(Cl)C=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Lumigen APS-5 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Lumigen APS-5
Cat. No.:
HY-112832
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