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LysoTracker Yellow HCK 123 

Cat. No.: HY-D1694
Handling Instructions

LysoTracker Yellow HCK 123 is a potent yellow membrane-permeable fluorescent probe. LysoTracker Yellow HCK 123 is a weakly basic amine that selectively accumulates in cellular compartments with low luminal pH. (λex=465 nm, λem=535 nm).

For research use only. We do not sell to patients.

LysoTracker Yellow HCK 123 Chemical Structure

LysoTracker Yellow HCK 123 Chemical Structure

CAS No. : 1064123-31-4

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Description

LysoTracker Yellow HCK 123 is a potent yellow membrane-permeable fluorescent probe. LysoTracker Yellow HCK 123 is a weakly basic amine that selectively accumulates in cellular compartments with low luminal pH. (λex=465 nm, λem=535 nm)[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
LysoTracker Yellow HCK 123 assay[1] (to quantify GAPDH):
1. Culture EMT6 cells in 8-well cell culture slides. Incubate the cells according to your normal protocol.
2. Add TONS504 per well to make the final concentration at 30 µg/mL.Incubate cells at 37 °C for 4 hours.
3. Add LysoTracker Yellow HCK 123 per well to make the final concentration at 50 μM. Incubate cells at 37 °C, 5% CO2, for 30 minutes.
4. Analyze samples on a confocal laser scanning microscope.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

364.40

Formula

C16H24N6O4

CAS No.
SMILES

CN(CCNC(CCCCCNC1=CC=C(C2=NON=C12)[N+]([O-])=O)=O)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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LysoTracker Yellow HCK 123
Cat. No.:
HY-D1694
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