LysoTracker Yellow HCK 123
Based on 1 publication(s) in Google Scholar
LysoTracker Yellow HCK 123 is a potent yellow membrane-permeable fluorescent probe. LysoTracker Yellow HCK 123 is a weakly basic amine that selectively accumulates in cellular compartments with low luminal pH.
For research use only. We do not sell to patients.
- Purity: 96.01%
- CAS No.: 1064123-31-4
- Formula: C16H24N6O4
- Molecular Weight:364.40
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) LysoTracker Yellow HCK 123
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Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
LysoTracker Yellow HCK 123 assay[1][2]:
1. Culture cells in 8-well cell culture slides. Incubate the cells according to your normal protocol.
2. Washing cells with PBS.
3. Add LysoTracker Yellow HCK 123 per well to make the final concentration at 0.1-50 μM. Incubate cells at 37 °C, 5% CO2, for 30 minutes.
4. Analyze samples on a confocal laser scanning microscope.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1064123-31-4
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Appearance Solid
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Molecular Weight 364.40
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Formula C16H24N6O4
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Color Yellow to orange
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SMILES
CN(CCNC(CCCCCNC1=CC=C(C2=NON=C12)[N+]([O-])=O)=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Autophagy
PRKN-IMMT/MIC60 axis promotes myocardial ischemia-reperfusion injury via lysosomal degradation of GPX4. [Abstract]2026 May 14. PMID: 42132226
Solvent & Solubility
DMSO : 62.5 mg/mL (171.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Desclés J, et, al. New tools for labeling silica in living diatoms. New Phytol. 2008;177(3):822-829. [Content Brief]
[2]. Jana Starigazdová, et al. In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: the internalization pathway. Eur J Pharm Sci2020 Feb 15:143:105168. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7442 mL | 13.7212 mL | 27.4424 mL | 68.6059 mL |
| 5 mM | 0.5488 mL | 2.7442 mL | 5.4885 mL | 13.7212 mL | |
| 10 mM | 0.2744 mL | 1.3721 mL | 2.7442 mL | 6.8606 mL | |
| 15 mM | 0.1829 mL | 0.9147 mL | 1.8295 mL | 4.5737 mL | |
| 20 mM | 0.1372 mL | 0.6861 mL | 1.3721 mL | 3.4303 mL | |
| 25 mM | 0.1098 mL | 0.5488 mL | 1.0977 mL | 2.7442 mL | |
| 30 mM | 0.0915 mL | 0.4574 mL | 0.9147 mL | 2.2869 mL | |
| 40 mM | 0.0686 mL | 0.3430 mL | 0.6861 mL | 1.7151 mL | |
| 50 mM | 0.0549 mL | 0.2744 mL | 0.5488 mL | 1.3721 mL | |
| 60 mM | 0.0457 mL | 0.2287 mL | 0.4574 mL | 1.1434 mL | |
| 80 mM | 0.0343 mL | 0.1715 mL | 0.3430 mL | 0.8576 mL | |
| 100 mM | 0.0274 mL | 0.1372 mL | 0.2744 mL | 0.6861 mL |
LysoTracker Yellow HCK 123 Related Classifications
HY-D1694 Related Classifications
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