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Cat. No.: HY-143468

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MEK-IN-5

MEK-IN-5 is a potent MEK inhibitor and NO donor. MEK-IN-5 significantly reduces the levels of pMEK and pERK in a dose-dependent and time-dependent manner. MEK-IN-5 induces apoptosis in MDA-MB-231 cells.

For research use only. We do not sell to patients.

CAS No.: 2417022-06-9
Formula: C₂₉H₂₇FN₄O₁₀S₂
Molecular Weight:674.67

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (270 KB)

MEK-IN-5 Chemical Structure

CAS No.: 2417022-06-9

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All MEK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	1.35 μM Compound: 18h	Antiproliferative activity against human A549 cells harboring KRAS G12S mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay Antiproliferative activity against human A549 cells harboring KRAS G12S mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
HCT-116	IC₅₀	0.64 μM Compound: 18h	Antiproliferative activity against human HCT116 cells harboring KRAS G13D mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay Antiproliferative activity against human HCT116 cells harboring KRAS G13D mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
L02	IC₅₀	5.62 μM Compound: 18h	Cytotoxicity against human HL7702 cells assessed as reduction in cell viability after 96 hrs by Celltiter-Glo assay Cytotoxicity against human HL7702 cells assessed as reduction in cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
MDA-MB-231	IC₅₀	0.034 μM Compound: 18h	Antiproliferative activity against human MDA-MB-231 cells harboring KRAS G13D/BRAF G464V mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay Antiproliferative activity against human MDA-MB-231 cells harboring KRAS G13D/BRAF G464V mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
Vero	IC₅₀	21.07 μM Compound: 18h	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 96 hrs by Celltiter-Glo assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]

In Vitro

MEK-IN-5 (compound 18h) shows anti-proliferation activities for different tumor cells and low toxicity for normal cells^[1].
MEK-IN-5 (0.1, 1, 10 µM; 1, 2, 4, 6 h) decreases the expression level of pMEK and pERK in a dose-dependent and time-dependent manner^[1].
MEK-IN-5 (1, 10 µM; 24 h) induces apoptosis in MDA-MB-231 cells^[1].
MEK-IN-5 (100 µM; 2h) significantly induce NO release in HCT116 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

MDA-MB-231, HCT116, A549, Vero, HL7702 cells
Concentration:
Incubation Time:
Result:

Showed anti-proliferation activities in MDA-MB-231, HCT116, A549, Vero, HL7702 cells with IC₅₀s of 0.034, 0.64, 1.35, 21.07, 5.62 µM, respectively.

Western Blot Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

0.1, 1, 10 µM
Incubation Time:

1, 2, 4, 6 h
Result:

Decreased the expression level of pMEK and pERK in a dose-dependent and time-dependent manner.

Apoptosis Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

1, 10 µM
Incubation Time:

24 h
Result:

Induced apoptosis in MDA-MB-231 cells.

Chemical Information

CAS No. 2417022-06-9
Molecular Weight 674.67
Formula C₂₉H₂₇FN₄O₁₀S₂
SMILES

O=C1OC2=C(C(C)=C1CC3=C(F)C(NS(=O)(NC)=O)=CC=C3)C=CC(OCCCOC4=NO[N+]([O-])=C4S(=O)(C5=CC=CC=C5)=O)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wang C, et al. Hybrids of MEK inhibitor and NO donor as multitarget antitumor drugs. Eur J Med Chem. 2020; 196:112271. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References

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MEK-IN-5

All MEK Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

MEK-IN-5 Related Classifications

HY-143468 Related Classifications