Mitochondrial penetrating peptide
Mitochondrial penetrating peptide is a peptide sequence (FrFKFrFK-CONH2) that selectively transports cargo into mitochondria. Mitochondrial penetrating peptide possesses special physicochemical properties, enabling it to selectively translocate dinuclear Ru (II) polypyridine complexes into mitochondria of living mammalian cells without the aid of solvents or membrane permeabilization treatments, thus achieving precise mitochondrial localization and enrichment of the complexes while excluding their distribution in the nucleus. Mitochondrial penetrating peptide enables dynamic monitoring of mitochondrial oxygen concentration and ROS production in living mammalian cells via changes in the luminescence lifetime of the coupled Ru (II) complex.
For research use only. We do not sell to patients.
- Formula: C60H87N17O8
- Molecular Weight:1174.44
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Mitochondrial penetrating peptides can selectively target conjugated dinuclear ruthenium (II) polypyridine complexes to the mitochondria of living HeLa cells, enabling precise organelle localization, while exhibiting extremely low cytoplasmic background and no nuclear accumulation[1].
Mitochondrial penetrating peptide enables the rapid uptake of conjugated dinuclear ruthenium (II) polypyridine complexes by live HeLa and CHO cells in a concentration- and temperature-dependent manner, and this membrane penetration process is energy-dependent[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 1174.44
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Formula C60H87N17O8
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Sequence
Phe-d{Arg}-Phe-Lys-Phe-d{Arg}-Phe-Lys-NH2
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Sequence Shortening
F-d{Arg}-FKF-d{Arg}-FK-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Mitochondrial penetrating peptide Related Classifications
HY-P11743 Related Classifications
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