2. Metabolic Enzyme/Protease
  3. Ser/Thr Protease
  4. N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide  (Synonyms: Ac-IEPD-pNA)

Cat. No.: HY-P3236 Pureza: 99.77%
COA
SDS
Instrucciones de manejo Technical Support

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a colorimetric peptide substrate of granzyme B that allows accurate measurement of granzyme B activity.

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N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide Estructura química

No. CAS : 216757-29-8

Tamaño Precio Stock Cantidad
5 mg En stock
10 mg En stock
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100 mg   Obtener un presupuesto  

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Based on 1 publication(s) in Google Scholar

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide Related Antibodies

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Descripciòn

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a colorimetric peptide substrate of granzyme B that allows accurate measurement of granzyme B activity[1].

In Vitro

Cytosols are standardised to 100 μg of total protein and incubated with granzyme B from CG in different amounts adjusted to a total volume of 30 μL in 96-well flat bottom microtiter plates for 30 min at 20 °C. Following incubation with granule isolate samples, 0.4 μL N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) (0.2 mM) and 70 μL of assay buffer (50 mM Hepes pH 7.4, 100 mM NaCl, 1 mM EDTA, 0.1 % NP-40, 10 % glycerol and 10 mM DTT) is added and incubated for 30 min at 37 °C. Absorption is measured at 405 nm in a microtiter plate photometry reader[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide Related Antibodies
Peso molecular

634.63

Fòrmula

C28H38N6O11
No. CAS
Appearance

Solid

Color

White to off-white

Sequence

Ac-Ile-Glu-Pro-Asp-{pNA}
Sequence Shortening

Ac-IEPD-{pNA}
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvente y solubilidad
In Vitro: 

DMSO : 100 mg/mL (157.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5757 mL 7.8786 mL 15.7572 mL
5 mM 0.3151 mL 1.5757 mL 3.1514 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureza y Documentación

Purity: 99.77%

SDS (394 KB)

COA (257 KB) LCMS (105 KB) Elemental Analysis Report (116 KB)

Instrucciones de manejo (2659 KB)
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5757 mL 7.8786 mL 15.7572 mL 39.3930 mL
5 mM 0.3151 mL 1.5757 mL 3.1514 mL 7.8786 mL
10 mM 0.1576 mL 0.7879 mL 1.5757 mL 3.9393 mL
15 mM 0.1050 mL 0.5252 mL 1.0505 mL 2.6262 mL
20 mM 0.0788 mL 0.3939 mL 0.7879 mL 1.9697 mL
25 mM 0.0630 mL 0.3151 mL 0.6303 mL 1.5757 mL
30 mM 0.0525 mL 0.2626 mL 0.5252 mL 1.3131 mL
40 mM 0.0394 mL 0.1970 mL 0.3939 mL 0.9848 mL
50 mM 0.0315 mL 0.1576 mL 0.3151 mL 0.7879 mL
60 mM 0.0263 mL 0.1313 mL 0.2626 mL 0.6566 mL
80 mM 0.0197 mL 0.0985 mL 0.1970 mL 0.4924 mL
100 mM 0.0158 mL 0.0788 mL 0.1576 mL 0.3939 mL
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N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide216757-29-8Ac-IEPD-pNASer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesInhibitorinhibitorinhibit

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Nombre del producto:
N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide
Cat. No.:
HY-P3236
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