N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide
Based on 1 Customer Validation
N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a colorimetric peptide substrate of granzyme B that allows accurate measurement of granzyme B activity.
For research use only. We do not sell to patients.
- Purity: 99.77%
- CAS No.: 216757-29-8
- Formula: C28H38N6O11
- Molecular Weight:634.63
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Cytosols are standardised to 100 μg of total protein and incubated with granzyme B from CG in different amounts adjusted to a total volume of 30 μL in 96-well flat bottom microtiter plates for 30 min at 20 °C. Following incubation with granule isolate samples, 0.4 μL N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) (0.2 mM) and 70 μL of assay buffer (50 mM Hepes pH 7.4, 100 mM NaCl, 1 mM EDTA, 0.1 % NP-40, 10 % glycerol and 10 mM DTT) is added and incubated for 30 min at 37 °C. Absorption is measured at 405 nm in a microtiter plate photometry reader[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 216757-29-8
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Appearance Solid
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Molecular Weight 634.63
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Formula C28H38N6O11
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Color White to off-white
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Synonyms
Ac-IEPD-pNA
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Sequence
Ac-Ile-Glu-Pro-Asp-{pNA}
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Sequence Shortening
Ac-IEPD-{pNA}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (157.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5757 mL | 7.8786 mL | 15.7572 mL | 39.3930 mL |
| 5 mM | 0.3151 mL | 1.5757 mL | 3.1514 mL | 7.8786 mL | |
| 10 mM | 0.1576 mL | 0.7879 mL | 1.5757 mL | 3.9393 mL | |
| 15 mM | 0.1050 mL | 0.5252 mL | 1.0505 mL | 2.6262 mL | |
| 20 mM | 0.0788 mL | 0.3939 mL | 0.7879 mL | 1.9697 mL | |
| 25 mM | 0.0630 mL | 0.3151 mL | 0.6303 mL | 1.5757 mL | |
| 30 mM | 0.0525 mL | 0.2626 mL | 0.5252 mL | 1.3131 mL | |
| 40 mM | 0.0394 mL | 0.1970 mL | 0.3939 mL | 0.9848 mL | |
| 50 mM | 0.0315 mL | 0.1576 mL | 0.3151 mL | 0.7879 mL | |
| 60 mM | 0.0263 mL | 0.1313 mL | 0.2626 mL | 0.6566 mL | |
| 80 mM | 0.0197 mL | 0.0985 mL | 0.1970 mL | 0.4924 mL | |
| 100 mM | 0.0158 mL | 0.0788 mL | 0.1576 mL | 0.3939 mL |
N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide Related Classifications
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