N-Acetyl-L-cysteine ethyl ester
Based on 13 publication(s) in Google Scholar
N-Acetyl-L-cysteine ethyl ester is an esterified form of N-acetyl-L-cysteine (NAC). N-Acetyl-L-cysteine ethyl ester exhibits enhanced cell permeability, and produce NAC and cysteine. N-Acetyl-L-cysteine ethyl ester increases circulating hydrogen sulfide (H2S) and can be used as an H2S producer. N-Acetyl-L-cysteine ethyl ester has the potential to substitute NAC as a mucolytic agent, and as a GSH-related antioxidant.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 59587-09-6
- Formula: C7H13NO3S
- Molecular Weight:191.25
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) N-Acetyl-L-cysteine ethyl ester
More- Signal Transduct Target Ther. 2023 Sep 25;8(1):366. [Abstract]
- Autophagy. 2026 Jun 16:1-20. [Abstract]
- Pharmacol Res. 2024 Nov:209:107420. [Abstract]
- Phytomedicine. 2025 Apr:139:156512. [Abstract]
- Cell Rep. 2024 Nov 10;43(11):114915. [Abstract]
- Drug Des Devel Ther. 2025 Jul 22:19:6219-6233. [Abstract]
- Bioorg Chem. 2025 Sep 16:165:109002. [Abstract]
- Cell Signal. 2026 Jun:142:112454. [Abstract]
- Biochim Biophys Acta Mol Cell Res. 2024 Jan;1871(1):119603. [Abstract]
- Cancer Med. 2025 May;14(9):e70855. [Abstract]
- Mol Carcinog. 2025 Apr;64(4):652-667. [Abstract]
- Biochem Biophys Res Commun. 2026 May 26:827:154038. [Abstract]
- Research Square Preprint. 2023 Jun 14.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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Cell Proliferation/Viability Assay
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Others
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In Vivo Efficacy Study
Biological Activity
N-Acetyl-L-cysteine ethyl ester is a an esterified form of N-acetyl-L-cysteine (NAC). N-Acetyl-L-cysteine ethyl ester exhibits enhanced cell permeability, and produce NAC and cysteine. N-Acetyl-L-cysteine ethyl ester increases circulating hydrogen sulfide (H2S) and can be used as an H2S producer. N-Acetyl-L-cysteine ethyl ester has the potential to substitute NAC as a mucolytic agent, and as a GSH-related antioxidant[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 59587-09-6
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Appearance Solid
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Molecular Weight 191.25
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Formula C7H13NO3S
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Color White to off-white
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SMILES
SC[C@@H](C(OCC)=O)NC(C)=O
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Synonyms
N-Acetylcysteine ethyl ester; NACET
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (13)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Non-small cell lung cancers (NSCLCs) oncolysis using coxsackievirus B5 and synergistic DNA-damage response inhibitors. [Abstract]2023 Sep 25;8(1):366. PMID: 37743418 -
Autophagy
Porcine reproductive and respiratory syndrome virus hijacks the non-canonical enzymatic function of PHGDH to arrest autophagic flux for viral replication. [Abstract]2026 Jun 16:1-20. PMID: 42260976 -
Pharmacol Res
Developing patient-derived organoids to demonstrate JX24120 inhibits SAMe synthesis in endometrial cancer by targeting MAT2B. [Abstract]2024 Nov:209:107420. PMID: 39293586
N-Acetyl-L-cysteine ethyl ester purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Nov:209:107420. [Abstract]
Cell viability was determined in response to ROS scavenger NAC (N-Acetyl-L-cysteine ethyl ester).
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Phytomedicine
EGCG inhibits tobacco smoke-promoted proliferation of lung cancer cells through targeting CCL5. [Abstract]2025 Apr:139:156512. PMID: 40010030
N-Acetyl-L-cysteine ethyl ester purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Apr:139:156512. [Abstract]
Inhibition of ROS generation can reverse CCL5-induced lung cancer cell proliferation. H1299 and H226 cells were treated with CCL5 protein and then with NAC (N-Acetyl-L-cysteine ethyl ester). Cell proliferation was detected by EdU staining.
N-Acetyl-L-cysteine ethyl ester purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Apr:139:156512. [Abstract]
Inhibition of ROS generation can reverse CCL5-induced lung cancer cell proliferation. H12996 cells were treated with CCL5 protein and then with NAC (N-Acetyl-L-cysteine ethyl ester). Western blot analysis was used to detect the protein levels of Cyclin D1, PCNA, Nrf2, and Keap1.
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Cell Rep
Metabolic disruption exacerbates intestinal damage during sleep deprivation by abolishing HIF1α-mediated repair. [Abstract]2024 Nov 10;43(11):114915. PMID: 39527478
N-Acetyl-L-cysteine ethyl ester purchased from MedChemExpress. Usage Cited in: Cell Rep. 2024 Nov 10;43(11):114915. [Abstract]
ROS level in the colon of SD-5d, SD-5d+NAC (N-Acetyl-L-cysteine ethyl ester: 150 mg/kg), and control mice.
N-Acetyl-L-cysteine ethyl ester purchased from MedChemExpress. Usage Cited in: Cell Rep. 2024 Nov 10;43(11):114915. [Abstract]
Weight changes in SD-5d, SD-5d+NAC (N-Acetyl-L-cysteine ethyl ester: 150 mg/kg) and control mice.
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Drug Des Devel Ther
Magnesium Isoglycyrrhizinate Alleviates Alectinib-Induced Hepatotoxicity by Inhibiting Mitochondrial Damage-Mediated Pyroptosis. [Abstract]2025 Jul 22:19:6219-6233. PMID: 40727921 -
Bioorg Chem
Exploring the mechanism of Levistolide A in the treatment of lung cancer cells based on network analysis, molecular docking and experimental validation. [Abstract]2025 Sep 16:165:109002. PMID: 40974659 -
Cell Signal
2026 Jun:142:112454. PMID: 41765311 -
Biochim Biophys Acta Mol Cell Res
Modified 5-aminolevulinic acid photodynamic therapy induces cutaneous squamous cell carcinoma cell pyroptosis via the JNK signaling pathway. [Abstract]2024 Jan;1871(1):119603. PMID: 37805058 -
Cancer Med
Characterization of the Biochemical Recurrence Prediction Ability and Progression Correlation of Peroxiredoxins Family in Prostate Cancer Based on Integrating Single-Cell RNA-Seq and Bulk RNA-Seq Cohorts. [Abstract]2025 May;14(9):e70855. PMID: 40281661 -
Mol Carcinog
RNF7-Mediated ROS Targets Malignant Phenotype and Radiotherapy Sensitivity in Glioma With Different IDH1 Genotypes. [Abstract]2025 Apr;64(4):652-667. PMID: 39783768 -
Biochem Biophys Res Commun
2026 May 26:827:154038. PMID: 42214921 -
Solvent & Solubility
DMSO : 100 mg/mL (522.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 100 mg/mL (522.88 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
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- Italian - IT (393 KB)
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- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 5.2288 mL | 26.1438 mL | 52.2876 mL | 130.7190 mL |
| 5 mM | 1.0458 mL | 5.2288 mL | 10.4575 mL | 26.1438 mL | |
| 10 mM | 0.5229 mL | 2.6144 mL | 5.2288 mL | 13.0719 mL | |
| 15 mM | 0.3486 mL | 1.7429 mL | 3.4858 mL | 8.7146 mL | |
| 20 mM | 0.2614 mL | 1.3072 mL | 2.6144 mL | 6.5359 mL | |
| 25 mM | 0.2092 mL | 1.0458 mL | 2.0915 mL | 5.2288 mL | |
| 30 mM | 0.1743 mL | 0.8715 mL | 1.7429 mL | 4.3573 mL | |
| 40 mM | 0.1307 mL | 0.6536 mL | 1.3072 mL | 3.2680 mL | |
| 50 mM | 0.1046 mL | 0.5229 mL | 1.0458 mL | 2.6144 mL | |
| 60 mM | 0.0871 mL | 0.4357 mL | 0.8715 mL | 2.1786 mL | |
| 80 mM | 0.0654 mL | 0.3268 mL | 0.6536 mL | 1.6340 mL | |
| 100 mM | 0.0523 mL | 0.2614 mL | 0.5229 mL | 1.3072 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
N-Acetyl-L-cysteine ethyl ester Related Classifications
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