N-Succinimidyl-S-acetylthioacetate
Based on 1 publication(s) in Google Scholar
N-Succinimidyl S-acetylthioacetate (SATA) is a protein modifying agent. N-Succinimidyl S-acetylthioacetate adds sulfhydryl groups to proteins and other amine-containing molecules in a protected form. N-Succinimidyl-S-acetylthioacetate can thiolate antibodies, thereby promoting efficient attachment of antibodies to other molecules and exerting conjugation activity. N-Succinimidyl-S-acetylthioacetate can be used in immunoassays such as ELISA studies.
For research use only. We do not sell to patients.
- Purity: 99.56%
- CAS No.: 76931-93-6
- Formula: C8H9NO5S
- Molecular Weight:231.23
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Storage:
4°C, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) N-Succinimidyl-S-acetylthioacetate
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Biological Activity
N-Succinimidyl-S-acetylthioacetate (8 mg/mL；30 min) is used for thiolation of goat antibody[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 76931-93-6
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Appearance Solid
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Molecular Weight 231.23
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Formula C8H9NO5S
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Color White to yellow
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SMILES
O=C(ON1C(CCC1=O)=O)CSC(C)=O
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Synonyms
SATA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 100 mg/mL (432.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * The compound is unstable in solutions, freshly prepared is recommended.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[2]. Allen KJH, et al. Evaluation of N-Succinimidyl S-Acetylthioacetate Ligand for Radiolabeling of HumanizedAntibodies with 188Rhenium. Cancer Biother Radiopharm. 2018 Oct;33(8):349-355. [Content Brief]
[3]. Nanda S, et al. Thermostable alpha-amylase conjugated antibodies as probes for immunodetection in ELISA. J Immunoassay Immunochem. 2002;23(3):327-45. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3247 mL | 21.6235 mL | 43.2470 mL | 108.1175 mL |
| 5 mM | 0.8649 mL | 4.3247 mL | 8.6494 mL | 21.6235 mL | |
| 10 mM | 0.4325 mL | 2.1623 mL | 4.3247 mL | 10.8117 mL | |
| 15 mM | 0.2883 mL | 1.4416 mL | 2.8831 mL | 7.2078 mL | |
| 20 mM | 0.2162 mL | 1.0812 mL | 2.1623 mL | 5.4059 mL | |
| 25 mM | 0.1730 mL | 0.8649 mL | 1.7299 mL | 4.3247 mL | |
| 30 mM | 0.1442 mL | 0.7208 mL | 1.4416 mL | 3.6039 mL | |
| 40 mM | 0.1081 mL | 0.5406 mL | 1.0812 mL | 2.7029 mL | |
| 50 mM | 0.0865 mL | 0.4325 mL | 0.8649 mL | 2.1623 mL | |
| 60 mM | 0.0721 mL | 0.3604 mL | 0.7208 mL | 1.8020 mL | |
| 80 mM | 0.0541 mL | 0.2703 mL | 0.5406 mL | 1.3515 mL | |
| 100 mM | 0.0432 mL | 0.2162 mL | 0.4325 mL | 1.0812 mL |
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