NAMPT activator-9
NAMPT activator-9 (Compound DIPM) is an allosteric, non-competitive NAMPT activator, with an EC50 of 3.366 μM against hNAMPT. NAMPT activator-9 enhances the enzymatic activity of NAMPT via an allosteric, non-competitive mechanism. NAMPT activator-9 increases intracellular NAD+ levels. NAMPT activator-9 restores myotube diameter and reduces the expression of atrophy markers Atrogin-1 and MuRF1. NAMPT activator-9 is applicable to research related to muscle atrophy.
For research use only. We do not sell to patients.
- CAS No.: 931366-46-0
- Formula: C17H15N5O
- Molecular Weight:305.33
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
In Vitro
NAMPT activator-9 (60 min) is a potent allosteric activator of hNAMPT, with an EC50 of 3.366 μM[1].
NAMPT activator-9 (0.741-20 μM; 24 h) significantly increases the intracellular NAD+ level and NAD+/NADH ratio in fully differentiated C2C12 myotubes; after treatment with 2.222 μM for 24 h, the NAD+ level increases by approximately 2-fold[1].
NAMPT activator-9 (2.222-20 μM; 24 h) protects fully differentiated C2C12 myotubes against Dexamethasone (HY-14648)-induced atrophy by reducing the expression of atrophy markers[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
-
Cell Line:Fully differentiated C2C12 myotubes
-
Concentration:0.247-4860 μM
-
Incubation Time:24 h
-
Result:Showed no obvious toxicity in C2C12 myotubes up to 180 μM, with an IC50 value of 1.98 mM.
-
Cell Line:Fully differentiated C2C12 myotubes (dexamethasone-induced atrophy model)
-
Concentration:2.222-20 μM (co-incubated with 10 μM dexamethasone)
-
Incubation Time:24 h
-
Result:Nearly completely abolished Dexamethasone-induced upregulation of Atrogin-1 and MuRF1 expression.
Reversed dexamethasone-induced reduction of MyHC expression, with significant recovery at all tested concentrations.
Chemical Information
-
CAS No. 931366-46-0
-
Molecular Weight 305.33
-
Formula C17H15N5O
-
SMILES
O=C(N1CC2=C(C=CC=C2)CC1)C3=CC=C(C4=NNN=N4)C=C3
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
NAMPT activator-9 Related Classifications
HY-182062 Related Classifications
Powered by Bioz